A novel mutagenesis method generating high yields of closed circular mutant DNA with one primer per mutant

Molecular Biotechnology

Volumn 30, Issue 1, 2005, Pages 21-30

  Erdogan, Eda ^a   Jones, Rachel J ^a   Matzlin, P ^a   Hanna, Michael H ^a   Smith, Susan M E ^a   Salerno, John C ^a  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

Linear amplification; Site directed mutagenesis

Indexed keywords

CELLS; CLONING; DNA; ENZYMES;

LINEAR AMPLIFICATION; SINGLE-STRANDED COPIES; SITE-DIRECTED MUTAGENESIS; THERMOPHILIC POLYMERASE;

MUTAGENESIS;

DNA POLYMERASE; DOUBLE STRANDED DNA; PLASMID DNA; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; PRIMER DNA; SINGLE STRANDED DNA;

ARTICLE; CONTROLLED STUDY; DNA REPAIR; DNA SYNTHESIS; GENE DELETION; GENE INSERTION; LINEAR SYSTEM; MOLECULAR CLONING; MUTANT; NONHUMAN; POINT MUTATION; POLYMERASE CHAIN REACTION; THERMOPHILIC BACTERIUM; ANIMAL; CHEMISTRY; GENE EXPRESSION REGULATION; HUMAN; METHODOLOGY; PLASMID; SITE DIRECTED MUTAGENESIS;

ANIMALS; DNA PRIMERS; HUMANS; MUTAGENESIS, INSERTIONAL; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; POINT MUTATION; POLYMERASE CHAIN REACTION; SEQUENCE DELETION;

EID: 26244441335     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (5)

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