An improved method for the purification of rat liver-type fatty acid binding protein from Escherichia coli

Protein Expression and Purification

Volumn 44, Issue 1, 2005, Pages 23-31

  Velkov, Tony ^a   Chuang, Sara ^a   Prankerd, Richard ^a   Sakellaris, Harry ^b   Porter, Christopher J H ^a   Scanlon, Martin J ^a  

^a MONASH UNIVERSITY   (Australia)

^b MONASH UNIVERSITY   (Australia)

Author keywords

Intracellular lipid binding protein recombinant protein expression; Liver fatty acid binding protein

Indexed keywords

FATTY ACID BINDING PROTEIN; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; LIVER; METABOLISM; METHODOLOGY; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; RAT;

ANIMALS; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FATTY ACID-BINDING PROTEINS; LIVER; RATS; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS;

EID: 26044469044     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.04.006     Document Type: Article

References (35)

1. L. Banaszak, N. Winter, Z. Xu, D.A. Bernlohr, S. Cowan, and T.A. Jones Lipid-binding proteins: a family of fatty acid and retinoid transport proteins Adv. Protein Chem. 45 1994 89 151
2. M.E. Newcomer, R.S. Jamison, and D.E. Ong Structure and function of retinoid-binding proteins Subcell. Biochem. 30 1998 53 80
3. A.M. Gallegos, B.P. Atshaves, S.M. Storey, O. Starodub, A.D. Petrescu, H. Huang, A.L. McIntosh, G.G. Martin, H. Chao, A.B. Kier, and F. Schroeder Gene structure, intracellular localization, and functional roles of sterol carrier protein-2 Prog. Lipid Res. 40 2001 498 563
4. F. Schroeder, C.A. Jolly, T.H. Cho, and A. Frolov Fatty acid binding protein isoforms: structure and function Chem. Phys. Lipids 92 1998 1 25
5. A.W. Zimmerman, and J.H. Veerkamp New insights into the structure and function of fatty acid-binding proteins Cell. Mol. Life Sci. 59 2002 1096 1116
6. N.M. Bass, and J.A. Manning Tissue expression of three structurally different fatty acid binding proteins from rat heart muscle, liver, and intestine Biochem. Biophys. Res. Commun. 137 1986 929 935
7. R.K. Ockner, J.A. Manning, and J.P. Kane Fatty acid binding protein. Isolation from rat liver, characterization, and immunochemical quantification J. Biol. Chem. 257 1982 7872 7878
8. J.F. Glatz, G.J. Vander Vusse, and J.H. Veerkamp Fatty acid-binding proteins and their physiological significance News Physiol. Sci. 3 1988 41 44
9. J. Thompson, N. Winter, D. Terwey, J. Bratt, and L. Banaszak The crystal structure of the liver fatty acid-binding protein. A complex with two bound oleates J. Biol. Chem. 272 1997 7140 7150
10. B.P. Atshaves, S.M. Storey, A. Petrescu, C.C. Greenberg, O.I. Lyuksyutova, R. Smith III, and F. Schroeder Expression of fatty acid binding proteins inhibits lipid accumulation and alters toxicity in L cell fibroblasts Am. J. Physiol. Cell. Physiol. 283 2002 688 703
11. S.H. Vincent, and U. Muller-Eberhard A protein of the Z class of liver cytosolic proteins in the rat that preferentially binds heme J. Biol. Chem. 260 1985 14521 14528
12. H. Takikawa, and N. Kaplowitz Binding of bile acids, oleic acid, and organic anions by rat and human hepatic Z protein Arch. Biochem. Biophys. 251 1986 385 392
13. T. Kanda, T. Ono, Y. Matsubara, and T. Muto Possible role of rat fatty acid-binding proteins in the intestine as carriers of phenol and phthalate derivatives Biochem. Biophys. Res. Commun. 168 1990 1053 1058
14. T. Hubbell, W.D. Behnke, J.K. Woodford, and F. Schroeder Recombinant liver fatty acid binding protein interacts with fatty acyl-coenzyme A Biochemistry 33 1994 3327 3334
15. A.E. Thumser, J.E. Voysey, and D.C. Wilton The binding of lysophospholipids to rat liver fatty acid-binding protein and albumin Biochem. J. 301 1994 801 806
16. A. Frolov, and F. Schroeder Time-resolved fluorescence of intestinal and liver fatty acid binding proteins: role of fatty acyl CoA and fatty acid Biochemistry 36 1997 505 517
17. C. Wolfrum, T. Borchers, J.C. Sacchettini, and F. Spener Binding of fatty acids and peroxisome proliferators to orthologous fatty acid binding proteins from human, murine, and bovine liver Biochemistry 39 2000 1469 1474
18. J.B. Lowe, A.W. Strauss, and J.I. Gordon Expression of a mammalian fatty acid-binding protein in Escherichia coli J. Biol. Chem. 259 1984 2696 2704
19. J.B. Lowe, J.C. Sacchettini, M. Laposata, J.J. McQuillan, and J.I. Gordon Expression of rat intestinal fatty acid-binding protein in Escherichia coli. Purification and comparison of ligand binding characteristics with that of Escherichia coli-derived rat liver fatty acid-binding protein J. Biol. Chem. 262 1987 5931 5937
20. 13C NMR study J. Biol. Chem. 264 1989 2700 2710
21. A.F. Worrall, C. Evans, and D.C. Wilton Synthesis of a gene for rat liver fatty-acid-binding protein and its expression in Escherichia coli Biochem. J. 278 1991 365 368
22. E. Amann, B. Ochs, and K.J. Abel Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli Gene 69 1988 301 315
23. G.V. Richieri, R.T. Ogata, and A.M. Kleinfeld Equilibrium constants for the binding of fatty acids with fatty acid-binding proteins from adipocyte, intestine, heart, and liver measured with the fluorescent probe ADIFAB J. Biol. Chem. 269 1994 23918 23930
24. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature (London) 227 1970 680 685
25. H. Blum, H. Beier, and H.J. Gross Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels Electrophoresis 8 1987 93 99
26. T. Velkov, S. Chuang, J. Wielens, H. Sakellaris, W.N. Charman, C.J.H. Porter, and M.J. Scanlon The interaction of lipophilic drugs with intestinal fatty acid binding protein J. Biol. Chem. 280 2005 17769 17776
27. H.J. Motulsky, GraphPad Software Inc., San Diego, CA (1999). Available from:
28. K.R. Miller, and D.P. Cistola Titration calorimetry as a binding assay for lipid-binding proteins Mol. Cell. Biochem. 123 1993 29 37
29. D.H. Alpers, A.W. Strauss, R.K. Ockner, N.M. Bass, and J.I. Gordon Cloning of a cDNA encoding rat intestinal fatty acid binding protein Proc. Natl. Acad. Sci. USA 81 1984 313 317
30. N. Haunerland, G. Jagschies, H. Schulenberg, and F. Spener Fatty-acid-binding proteins. Occurrence of two fatty-acid-binding proteins in bovine liver cytosol and their binding of fatty acids, cholesterol, and other lipophilic ligands Hopper Seylers Z. Physiol. Chem. 365 1984 365 376
31. J.F. Glatz, and J.H. Veerkamp A radiochemical procedure for the assay of fatty acid binding by proteins Anal. Biochem. 132 1983 89 95
32. C.D. Kane, and D.A. Bernlohr A simple assay for intracellular lipid-binding proteins using displacement of 1-anilinonaphthalene 8-sulfonic acid Anal. Biochem. 233 1996 197 204
33. H. Huang, O. Starodub, A. McIntosh, B.P. Atshaves, G. Woldegiorgis, A.B. Kier, and F. Schroeder Liver fatty acid-binding protein colocalizes with peroxisome proliferator activated receptor alpha and enhances ligand distribution to nuclei of living cells Biochemistry 43 2004 2484 2500
34. J.I. Gordon, D.H. Alpers, R.K. Ockner, and A.W. Strauss The nucleotide sequence of rat liver fatty acid binding protein mRNA J. Biol. Chem. 258 1983 3356 3363
35. D.P. Cistola, M.T. Walsh, R.P. Corey, J.A. Hamilton, and P. Brecher Interactions of oleic acid with liver fatty acid binding protein: a carbon-13 NMR study Biochemistry 27 1988 711 717