Expression of recombinant MDA-BF-1 with a kinase recognition site and a 7-histidine tag for receptor binding and purification

Protein Expression and Purification

Volumn 44, Issue 1, 2005, Pages 58-64

  Liang, Albert K ^a   Liu, Jonathan ^a   Mao, Stephen A ^a   Siu, Vince S ^a   Lee, Yu Chen ^a   Lin, Sue Hwa ^a  

^a UNIVERSITY OF TEXAS   (United States)

Author keywords

Affinity chromatography; Bone metastasis related factor 1; Glycoprotein; IMAC; Insect cell expression system; PKA; Receptor

Indexed keywords

CYCLIC AMP DEPENDENT PROTEIN KINASE; EPIDERMAL GROWTH FACTOR RECEPTOR; HISTIDINE; HYBRID PROTEIN; MDA BF 1, HUMAN; MDA-BF-1, HUMAN; MEMBRANE PROTEIN; TUMOR PROTEIN;

ANIMAL; ARTICLE; CHEMISTRY; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOUSE; PROTEIN BINDING; TUMOR CELL LINE;

ANIMALS; CELL LINE, TUMOR; CYCLIC AMP-DEPENDENT PROTEIN KINASES; GENE EXPRESSION; HISTIDINE; HUMANS; MEMBRANE PROTEINS; MICE; NEOPLASM PROTEINS; PROTEIN BINDING; RECEPTOR, EPIDERMAL GROWTH FACTOR; RECOMBINANT FUSION PROTEINS;

INSECTA;

EID: 26044431905     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.03.025     Document Type: Article

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