Studying the synergistic damage effects induced by 1.8 GHz radiofrequency field radiation (RFR) with four chemical mutagens on human lymphocyte DNA using comet assay in vitro

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 578, Issue 1-2, 2005, Pages 149-157

  Baohong, Wang ^a   Jiliang, He ^a,b   Lifen, Jin ^a   Deqiang, Lu ^a   Wei, Zheng ^a   Jianlin, Lou ^a   Hongping, Deng ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b JIAXING UNIVERSITY   (China)

Author keywords

Bleomycin; DNA damage; Methyl methanesulfonate; Microwaves; Mitomycin C; Single cell gel electrophoresis (comet assay)

Indexed keywords

4 NITROQUINOLINE 1 OXIDE; ALKYLATING AGENT; BLEOMYCIN; CELL DNA; CHEMICAL MUTAGEN; MESYLIC ACID METHYL ESTER; MITOMYCIN C;

ADULT; ARTICLE; COMET ASSAY; CONTROLLED STUDY; DNA CROSS LINKING; DNA DAMAGE; GENOTOXICITY; HUMAN; HUMAN CELL; INCUBATION TIME; LYMPHOCYTE; MALE; OBSERVATION; PRIORITY JOURNAL; RADIATION EXPOSURE; RADIATION FIELD; RADIATION INJURY; RADIOFREQUENCY RADIATION; ULTRAVIOLET RADIATION;

4-NITROQUINOLINE-1-OXIDE; ADULT; BLEOMYCIN; COMET ASSAY; DNA; DRUG SYNERGISM; HUMANS; LYMPHOCYTES; MALE; METHYL METHANESULFONATE; MICROWAVES; MITOMYCIN; MUTAGENS;

EID: 25844484034     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrfmmm.2005.05.001     Document Type: Article

References (30)

1. L. Verschaeve, and A. Maes Genetic, carcinogenic and teratogenic effects of radiofrequency fields Mutat. Res. 410 1998 141 165
2. M.L. Meltz Radiofrequency exposure and mammalian cell toxicity, genotoxicity, and transformation Bioelectromagnetics Suppl. 6 2003 196 213
3. J.P. McNamee, P.V. Bellier, G.B. Gajda, B.F. Lavallée, L. Marro, E. Lemay, and A. Thansandote No evidence for genotoxic effects from 24 h exposure of human leukocytes to 1.9 GHz radiofrequency fields Radiat. Res. 159 2003 693 697
4. J.P. McNamee, P.V. Bellier, G.B. Gajda, S.M. Miller, E.P. Lemay, B.F. Lavallée, L. Marro, and A. Thansandote DNA damage and micronucleus induction in human lymphocytes after acute in vitro exposure to a 1.9 GHz continuous-wave radiofrequency field Radiat. Res. 158 2002 523 533
5. G.J. Hook, P. Zhang, I. Lagroye, L. Li, R. Higashikubo, E.G. Moros, W.L. Straube, W.F. Pickard, J.D. Baty, and J.L. Roti Roti Measurement of DNA damage and apoptosis in Molt-4 cells after in vitro exposure to radiofrequency radiation Radiat. Res. 161 2004 193 200
6. Vijayalaxmi, and G. Obe Controversial cytogenetic observations in mammalian somatic cells exposed to radiofrequency radiation Radiat. Res. 162 2004 481 496
7. M.L. Meltz, K.A. Walker, and D.N. Erwin Radiofrequency (mircrowave) radiation exposure of mammalian cells during UV-induced DNA repair synthesis Radiat. Res. 110 1987 255 266
8. V. Ciaravino, M.L. Meltz, and D.N. Erwin Absence of a synergistic effect between moderate-power radiofrequency electromagnetic radiation and adriamycin on cell-cycle progression and sister-chromatid exchange Bioelectromagnetics 12 1991 289 298
9. A. Maes, M. Collier, D. Slaets, and L. Verschaeve 954 MHz microwaves enhance the mutagenic properties of mitomycin C Environ. Mol. Mutagen. 28 1996 26 30
10. A. Maes, M. Collier, U.V. Gorp, S. Vandoninck, and L. Verachaeve Cytogenetic effects of 935.2 MHz (GSM) microwaves alone and in combination with mitomycin C Mutat. Res. 393 1997 151 156
11. P. Gos, B. Eicher, J. Kohli, and W.D. Heyer No mutagenic or recombinogenic effects of mobile phone fields at 900 MHz detected in the Yeast Saccharomyces cerevisiae Bioelectromagnetics 21 2000 515 523
12. A. Maes, M. Collier, and L. Verschaeve Cytogenetic effects of 900 MHz (GSM) microwaves on human lymphocytes Bioelectromagnetics 22 2001 91 96
13. L. Stronati, A. Testa, P. Villani, C. Marino, G.A. Lovisolo, D. Conti, F. Russo, A.M. Fresegna, and E. Cordelli Absence of genotoxicity in human blood cells exposed to 50 Hz magnetic fields as assessed by comet assay, chromosome aberration, micronucleus, and sister chromatid exchange analyses Bioelectromagnetics 25 2004 41 48
14. A. Markkanen, P. Penttinen, J. Naarala, J. Pelkonen, A.P. Sihvonen, and J. Juutilainen Apoptosis induced by ultraviolet radiation is enhanced by amplitude modulated radiofrequency radiation in mutant Yeast cells Bioelectromagnetics 25 2004 127 133
15. M.B. Zhang, J.L. He, L.F. Jin, and D.Q. Lu Study of low-intensity 2450 MHz microwave exposure enhancing the genotoxic effects of mitomycin C using micronucleus test and comet assay in vitro Biomed. Environ. Sci. 15 2002 283 290
16. R.R. Tice, E. Agurell, D. Anderson, B. Burlinson, A. Hartmann, H. Kobayashi, Y. Miyamae, E. Rojas, J.C. Ryu, and Y.F. Sasaki Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing Environ. Mol. Mutagen. 35 2000 206 221
17. R.S. Malyapa, E.W. Alhern, C. Bi, W.L. Straube, M. LaRegina, W.F. Pickard, and J.L. Roti Roti DNA damage in rat brain cells after in vito exposure to 2450 MHz electromagnetic radiation and various methods of euthanasia Radiat. Res. 149 1998 637 645
18. J.L. Phillips, O. Ivaschuk, T.I. Jones, R.A. Jones, M.C. Beachler, and W. Haggren DNA damage in Molt-4 T-lymphoblastoid cells exposed to cellular telephone radiofrequency fields in vitro Bioelectrochem. Bioenerg. 45 1998 103 110
19. R.R. Tice, G.G. Hook, M. Donner, D.I. McRee, and A.W. Guy Genotoxicity of radiofrequency signals. 1. Investigation of DNA damage and micronuclei induction in cultured human blood cells Bioelelectromagnetics 23 2002 113 126
20. G. Frenzilli, E. Bosco, and R. Barale Validation of single cell gel assay in human leukocytes with 18 reference compounds Mutat. Res. 468 2000 93 108
21. Y. Miyamae, K. Zaizen, K. Ohara, Y. Mine, and Y.F. Sasaki Detection of DNA lesions induced by chemical mutagens by the single cell gel electophoresis (comet) assay. 1. Relationship between the onset of DNA damage and the characteristics of mutagens Mutat. Res. 393 1997 99 106
22. C.L. Garcia, M. Carloni, N.P. De la Peña, E. Fonti, and F. Palitti Detection of DNA primary damage by premature chromosome condensation in human peripheral blood lymphocytes treated with methyl methanesulfonate Mutagenesis 16 2001 121 125
23. N. Kuster, Summary of Dosimetric Functions for sXc-1800 System, The manual provided by Agilent CO., Switzerland, 2002.
24. N.P. Singh, M.T. Mc Coy, R.R. Tice, and E.L. Schneider A simple technique for quantitation of low levels of DNA damage in individual cells Exp. Cell Res. 175 1988 184 191
25. H. Lai, and N.P. Singh Acute low-intensity microwave exposure increases DNA single-strand breaks in rat brain cells Bioelectromagnetics 16 1995 207 210
26. Vijayalaxami, B.Z. Leals, M. Szilagyi, T.J. Prihoda, and M.L. Meltz Primary DNA damage in human blood lymphocytes exposed in vitro to 2450 MHz radiofrequency radiation Radiat. Res. 153 2000 479 486
27. I. Trosic, I. Busljeta, and B. Modlic Investigation of the genotoxic effect of microwavw irradiation in rat bone marrow cells: in vitro exposure Mutagenesis 19 2004 361 364
28. C. Bernstein, H. Bernstein, C.M. Payne, and H. Garewal DNA repair/pro-apoptotic dual-role proteins in five major DNA repair pathways: fail-safe protection against carcinogenesis Mutat. Res. 511 2002 145 178
29. P. Daza, P. Escalza, S. Mateos, F. Cortés, and C. Mitomycin 4-Nitroquinoline-1-oxide and ethyl methanesulfonate induce long-lived lesions in DNA which result in SCEs during successive cell cycles in human lymphocytes Mutat. Res. 270 1992 177 183
30. M. Hömme, H. Jacobi, U. Juhl-Stauss, and I. Witte Synergistic DNA damaging effects of 4-nitroquinoline-1-oxide and non-effective concentrations of methyl methanesulfonate in human fibroblast Mutat. Res. 461 2000 211 219