Analysis of COX2 mutants reveals cytochrome oxidase subassemblies in yeast

Biochemical Journal

Volumn 390, Issue 3, 2005, Pages 703-708

  Horan, Susannah ^a   Bourges, Ingrid ^a   Taanman, Jan Willem ^b   Meunier, Brigitte ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

Author keywords

Assembly; Blue native protein electrophoresis; COX2 mutant; Cytochrome c oxidase; Haem aa3; Yeast

Indexed keywords

ASSEMBLY; CATALYSIS; CELLS; MUTAGENESIS; OXYGEN; YEAST;

BLUE NATIVE PROTEIN ELECTROPHORESIS; COX2; CYTOCHROME C OXIDASE; HAEM AA3;

ENZYMES;

CYCLOOXYGENASE 2; CYTOCHROME C OXIDASE; MUTANT PROTEIN;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME ACTIVITY; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; WILD TYPE; YEAST;

ELECTRON TRANSPORT COMPLEX IV; MUTATION; PROTEIN SUBUNITS; SACCHAROMYCES CEREVISIAE;

EID: 25844448754     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20050598     Document Type: Article

References (24)

1. Shoubridge, E. A. (2001) Cytochrome c oxidase deficiency. Am. J. Med. Genet. 106, 46-52
2. Barrientos, A., Barros, M. H., Valnot, I., Rotig, A., Rustin, P. and Tzagoloff, A. (2002) Cytochrome oxidase in health and disease. Gene 286, 53-63
3. Wielburski, A. and Nelson, B. D. (1983) Evidence for the sequential assembly of cytochrome oxidase subunits in rat liver mitochondria. Biochem. J. 212, 829-834
4. Nijtmans, L. G. J., Taanman, J.-W., Muijsers, A. O., Speijer, D. and Van den Bogert, C. (1998) Assembly of cytochrome c oxidase in cultured human cells. Eur. J. Biochem. 254, 389-394
5. Williams, S. L., Valnot, I., Rustin, P. and Taanman, J.-W. (2004) Cytochrome c oxidase subassemblies in fibroblast cultures from patients carrying mutations in COX10, SC01, or SURF1. J. Biol. Chem. 279, 7462-7469
6. Dickinson, E. K., Adams, D. L., Schon, E. and Glerum, D. M. (2000) A human SC02 mutation helps define the role of Sco1p in the cytochrome oxidase assembly pathway. J. Biol. Chem. 275, 26780-26785
7. Bratton, M., Mills, D., Castleden, C. K., Hosler, J. and Meunier, B. (2003) Disease-related mutations in cytochrome c oxidase studied in yeast and bacterial models. Eur. J. Biochem. 270, 1-9
8. Meunier, B. (2001) Site-directed mutations in the mitochondrially-encoded subunits I and III of yeast cytochrome oxidase. Biochem. J. 354, 407-412
9. Church, C., Goehring, B., Forsha, D., Wazny, P. and Poyton, R. O. (2005) A role for Pet100p in the assembly of yeast cytochrome c oxidase. Interaction with a subassembly that accumulates in a pet100 mutant. J. Biol. Chem. 280, 1854-1863
10. Langer, T. (2000) AAA proteases: cellular machines for degrading membrane proteins. Trends Biochem. Sci. 25, 247-251
11. Tzagoloff, A. and Dieckmann, C. L. (1990) PET genes of Saccharomyces cerevisiae. Microbiol. Rev. 54, 211-225
12. Brown, S., Colson, A.-M., Meunier, B. and Rich, P. R. (1993) Rapid screening of cytochromes of respiratory mutants of Saccharomyces cerevisiae - application to the selection of strains containing novel forms of cytochrome c oxidase. Eur. J. Biochem. 213, 137-145
13. Meunier, B. and Rich, P. R. (1998) Second-site reversion analysis is not a reliable method to determine distance in membrane proteins: an assessment using mutations in yeast cytochrome c oxidase subunits I and II. J. Mol. Biol. 283, 727-730
14. Meunier, B., Lemarre, P. and Colson, A.-M. (1993) Genetic screening in Saccharomyces cerevisiae for large numbers of mitochondrial point mutations which affect structure and function of catalytic subunits of cytochrome-c oxidase. Eur. J. Biochem. 213, 129-135
15. Conde, J. and Fink, G. R. (1976) A mutant of Saccharomyces cerevisiae defective for nuclear fusion. Proc. Natl. Acad. Sci. U.S.A. 73, 3651-3655
16. Bonnefoy, N. and Fox, T. D. (2001) Genetic transformation of Saccharomyces cerevisiae mitochondria. Methods Cell Biol. 65, 381-396
17. Meunier, B. and Taanman, J.-W. (2002) Mutations of cytochrome c oxidase subunits 1 and 3 in Saccharomyces cerevisiae: assembly defect and compensation. Biochim. Biophys. Acta 1554, 101-107
18. Fisher, N., Castleden, C. K., Bourges, I., Brasseur, G., Dujardin, G. and Meunier, B. (2004) Human disease-related mutations in cytochrome b studied in yeast. J. Biol. Chem. 279, 12951-12958
19. Schagger, H. (1995) Quantification of oxidative phosphorylation enzymes after blue native electrophoresis and two-dimensional resolution: normal complex I protein amounts in Parkinson's disease conflict with reduced catalytic activities. Electrophoresis 16, 763-770
20. Taanman, J.-W. and Capaldi, R. A. (1993) Subunit Via of yeast cytochrome c oxidase is not necessary for assembly of the enzyme complex but modulates the enzyme activity. Isolation and characterization of the nuclear-coded gene. J. Biol. Chem. 268, 18754-18761
21. Zerbetto, E. V. L. and Dabbeni-Sala, F. (1997) Quantification of muscle mitochondrial oxidative phosphorylation enzymes via histochemical staining of blue native polyacrylamide gels. Electrophoresis 18, 2059-2064
22. Taanman, J.-W. and Capaldi, R. A. (1992) Purification of yeast cytochrome c oxidase with a subunit composition resembling the mammalian enzyme. J. Biol. Chem. 267, 22481-22485
23. Glerum, D. M. and Tzagoloff, A. (1997) Submitochondrial distributions and stabilities of subunits 4, 5 and 6 of yeast cytochrome oxidase in assembly defective mutants. FEBS Lett. 412, 410-414
24. Hiser, L. and Hosler, J. P. (2002) Heme A is not essential for assembly of the subunits of cytochrome c oxidase of Rhodobacter sphaeroides. J. Biol. Chem. 276, 45403-45407