Utility of confocal laser scanning microscopy (CLSM): With reference to interpretation in immunostaining

Acta Histochemica et Cytochemica

Volumn 38, Issue 4, 2005, Pages 267-271

  Ogane, Naoki ^a   Yasuda, Masanori ^b   Hayashi, Hiroyuki ^a   Kameda, Yoichi ^a   Minematsu, Takeo ^b   Itoh, Johbu ^b   Osamura, R Yoshiyuki ^b  

^a KANAGAWA CANCER CENTER   (Japan)

^b TOKAI UNIVERSITY   (Japan)

Author keywords

Confocal laser scanning microscopy (CLSM); Immunohistochemistry (IHC)

Indexed keywords

DIAMINOBENZIDINE; EOSIN; GLUCOSE TRANSPORTER 1; HEMATOXYLIN;

ARTICLE; CELL MEMBRANE; CELL STRUCTURE; CELLULAR DISTRIBUTION; CONFOCAL LASER MICROSCOPY; CYTODIAGNOSIS; CYTOLOGY; CYTOPLASM; DIAGNOSTIC ACCURACY; DIAGNOSTIC PROCEDURE; DIAGNOSTIC TEST; ENDOMETRIOID CARCINOMA; GLUCOSE TRANSPORT; HISTOLOGY; HUMAN; IMMUNOHISTOCHEMISTRY; IMMUNOLOCALIZATION; NONHUMAN; PAPANICOLAOU TEST; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; STAINING;

EID: 25644461072     PISSN: 00445991     EISSN: 13475800     Source Type: Journal    
DOI: 10.1267/ahc.38.267     Document Type: Article

References (16)

1. Haber, R. S., Weiser, K. R., Pritsker, A., Reder, I. and Burstein, D. E. (1997) GLUT1 glucose transporter expression in benign and malignant thyroid nodules. Thyroid 7; 363-367.
2. Itoh, J., Sanno, N., Matsuno, A., Itoh, Y., Watanabe, K. and Osamura, R. Y. (1997) Application of confocal laser scanning microscopy (CLSM) to visualize prolactin (PRL) and PRL mRNA in the normal and estrogen-treated rat pituitary glands using non-fluorescent probes. Microsc. Res. Tech. 39; 157-167.
3. Itoh, J., Kawai, K., Serizawa, A., Yasumura, K., Ogawa, K. and Osamura, R. Y. (2000) A new approach to three-dimensional reconstructed imaging of hormone-secreting cells and their microvessel environments in rat pituitary glands by confocal laser scanning microscopy. J. Histochem. Cytochem. 48; 569-578.
4. Itoh, J., Matsuno, A., Yamamoto, Y., Kawai, K., Serizawa, A., Watanabe, K., Itoh, Y. and Osamura, R. Y. (2001) Confocal laser scanning microscopic imaging of subcellular organelles, mRNA, protein products, and the mirovessel environment. Acta Histochem. Cytochem. 34; 285-297.
5. Itoh, J., Serizawa, A., Kawai, K., Ishii, Y., Teramoto, A. and Osamura, R. Y. (2003) Vascular networks and endothelial cells in the rat experimental pituitary glands and in the human pituitary adenomas. Microsc. Res. Tech. 60; 231-235.
6. Itoh, J., Umemura, S., Hasegawa, H., Yasuda, M., Takekoshi, S., Osamura, R. Y. and Watanabe, K. (2003) Simultaneous detection of DAB and methyl green signals on apoptotic nuclei by confocal laser scanning microscopy. Acta Histochem. Cytochem. 36; 367-376.
7. Joost, H. G. and Thorens, B. (2001) The extended GLUT-family of sugar/polyol transport facilitators: nomenclature, sequence characteristics, and potential function of its novel members. Mol. Membr. Biol. 18; 247-256.
8. Ogane, N., Yasuda, M., Kamoshida, S. and Osamura, R. Y. (2005) Significance of GLUT-1 expression as a differential and biological marker in thyroid gland tumors. Abstract: The 45th annual meeting of the Japan Society of Histochemistry and Cytochemistry. Acta Histochem. Cytochem. 38; 170.
9. Rudlowski, C., Moser, M., Becker, A. J., Rath, W., Buttner, R., Schroder, W. and Schurmann, A. (2004) GLUT1 mRNA and protein expression in ovarian borderline tumors and cancer. Oncology 66; 404-410.
10. Sato, S., Ito, K., Jiko, K., Konno, R., Yajima, A. and Sasano, H. (1998) Cytologic application of p53 overexpression using immunohistochemistry and confocal laser scanning microscopy. Acta Cytol. 42; 342-345.
11. Schönberger, J., Rüschoff, J., Grimm, D., Marienhagen, J., Rümmele, P., Meyringer, R., Kossmehl, P., Hofstaedter, F. and Eilles, C. (2002) Glucose transporter 1 gene expression is related to thyroid neoplasms with an unfavorable prognosis: an immunohistochemical study. Thyroid 12; 747-754.
12. Wang, B. Y., Kalir, T., Sabo, E., Sherman, D. E., Cohen, C. and Burstein, D. E. (2000) Immunohistochemical staining of GLUT1 in benign, hyperplastic, and malignant endometrial epithelia. Cancer 88; 2774-2781.
13. Yasuda, M., Itoh, J., Muramatsu, T., Shinozuka, T., Sasano, H., Tsutsumi, Y. and Osamura, R. Y. (1997) Subcellular organelle analysis applying confocal laser scanning microscopy to the steroid-producing epithelial ovarian tumor: a technical note. Pathol. Int. 47; 336-338.
14. 4) and dimethylnitrosamine (DMN). J. Histochem. Cytochem. 48; 1331-1339.
15. Yasuda, M., Itoh, J., Kotajima, S., Itoh, H., Murakami, M., Ogane, N. and Osamura, R. Y. (2004) Cytologic three-dimensional imaging for the interpretation of staining profiles: application of confocal laser scanning microscopy. Diagn. Cytopathol. 31; 166-168.
16. Younes, M., Lechago, L. V. and Lechago, J. (1996) Overexpression of the human erythrocyte glucose transporter occurs as a late event in human colorectal carcinogenesis and is associated with an increased incidence of lymph node metastases. Clin. Cancer Res. 2; 1151-1154.