Rapid detection and differentiation of the major mycoplasma contaminants in cell cultures using real-time PCR with SYBR green I and melting curve analysis

Microbiology and Immunology

Volumn 49, Issue 9, 2005, Pages 859-863

  Harasawa, Ryô ^a   Mizusawa, Hiroshi ^b   Fujii, Masashi ^c   Yamamoto, Junko ^c   Mukai, Hiroyuki ^c   Uemori, Takashi ^c   Asada, Kiyozo ^c   Kato, Ikunoshin ^c  

^a IWATE UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF BIOMEDICAL INNOVATION   (Japan)

^c TAKARA BIO INC   (Japan)

Author keywords

Melting curve; Mycoplasma; Real time PCR; SYBR Green I

Indexed keywords

FLUORESCENT DYE;

ANALYTIC METHOD; ARTICLE; BACTERIAL CELL; BACTERIUM CONTAMINATION; BACTERIUM DETECTION; CELL CULTURE; COLONY FORMING UNIT; CONTROLLED STUDY; MELTING POINT; MYCOPLASMA; NONHUMAN; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SPECIES DIFFERENTIATION; TEMPERATURE;

BACTERIA (MICROORGANISMS); MYCOPLASMA; MYCOPLASMATALES;

EID: 25444525161     PISSN: 03855600     EISSN: 13480421     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2005.tb03675.x     Document Type: Article

References (7)

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