G204D, a mutation that blocks the proton-conducting D-channel of the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides

Biochemistry

Volumn 44, Issue 38, 2005, Pages 12767-12774

  Han, Dan ^a   Morgan, Joel E ^a,b   Gennis, Robert B ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b Research Rensselaer Polytechnic Institute   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; ENZYME INHIBITION; FREE ENERGY; MUTAGENS; OXYGEN; PROTONS;

CYTOCHROME C OXIDASE; OXYGEN REDUCTION; PROTON PUMPING; TRANSMEMBRANE PROTON GRADIENT;

ENZYME KINETICS;

ASPARAGINE; ASPARTIC ACID; CYTOCHROME C OXIDASE; GLYCINE; ION CHANNEL; OXIDOREDUCTASE; OXYGEN; PROTON PUMP; RECOMBINANT ENZYME; WATER;

ARTICLE; CELL VACUOLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBUNIT; LIPID MEMBRANE; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTON TRANSPORT; RESPIRATION CONTROL; RHODOBACTER SPHAEROIDES; STEADY STATE;

ARACHIDONIC ACID; ASPARTIC ACID; BACTERIAL PROTEINS; ELECTRON TRANSPORT COMPLEX IV; GLYCINE; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OXIDATION-REDUCTION; PROTONS; RHODOBACTER SPHAEROIDES;

RHODOBACTER SPHAEROIDES;

EID: 25444491194     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi051141m     Document Type: Article

References (32)

1. Aagaard, A., and Brzezinski, P. (2001) Zinc Ions Inhibit Oxidation of Cytochrome c Oxidase by Oxygen, FEBS Lett. 494, 157-160.
2. Ferguson-Miller, S., and Babcock, G. T. (1996) Heme/Copper Terminal Oxidases, Chem. Rev. 7, 2889-2907.
3. Wikström, M. (2004) Cytochrome c Oxidase: 25 Years of the Elusive Proton Pump, Biochim. Biophys. Acta 1655, 241-247.
4. Michel, H. (1998) The Mechanism of Proton Pumping by Cytochrome c Oxidase, Proc. Natl. Acad. Sci. U.S.A. 95, 12819-12824.
5. Michel, H. (1999) Cytochrome c Oxidase: Catalytic Cycle and Mechanisms of Proton Pumping-A Discussion, Biochemistry 38, 15129-15140.
6. Iwata, S., Ostermeier, C., Ludwig, B., and Michel, H. (1995) Structure at 2.8 A Resolution of Cytochrome c Oxidase from Paracoccus denitrificans, Nature 376, 660-669.
7. Fetter, J. R., Qian, J., Shapleigh, J., Thomas, J. W., Garcia-Horsman, A., Schmidt, E., Hosler, J., Babcock, G. T., Gennis, R. B., and Ferguson-Miller, S. (1995) Possible Proton Relay Pathways in Cytochrome c Oxidase, Proc. Natl. Acad. Sci. U.S.A. 92, 1604-1608.
8. 3 Ubiquinol Oxidase of Escherichia coli: Second Site Mutations in Subunit I that Restore Proton Pumping in the Decoupled Mutant Asp135Asn, Biochemistry 34, 4428-4433.
9. Brädén, M., Tomson, F. L., Gennis, R. B., and Brzezinski, P. (2002) Identification of the Entry Point of the K-proton-Transfer Pathway in Cytochrome c Oxidase, Biochemistry 41, 10794-10798.
10. Wikström, M., Jasaitis, A., Backgren, C., Puustinen, A., and Verkhovsky, M. I. (2000) The Role of the D- and K-pathways of Proton Transfer in the Function of the Haem-copper Oxidases, Biochim. Biophys. Acta 1459, 514-520.
11. Rultenberg, M., Kannt, A., Bamberg, E., Fendler, K., and Michel, H. (2002) Reduction of Cytochrome c Oxidase by a Second Electron Leads to Proton Translocation, Nature 417, 99-102.
12. Konstantinov, A. A., Siletsky, S., Mitchell, D., Kaulen, A., and Gennis, R. B. (1997) The Roles of the Two Proton Input Channels in Cytochrome c Oxidase from Rhodobacter sphaeroides Probed by the Effects of Site-Directed Mutations on Time-Resolved Electrogenic Intraprotein Proton Transfer, Proc. Natl. Acad. Sci. U.S.A. 94, 9085-9090.
13. Fetter, J., Sharpe, M., Qian, J., Mills, D., Ferguson-Miller, S., and Nicholls, P. (1996) Fatty Acids Stimulate Activity and Restore Respiratory Control in a Proton Channel Mutant of Cytochrome c Oxidase, FEBS Lett. 393, 155-160.
14. 3 from Rhodobacter sphaeroides is Involved in Proton Uptake During the Reaction of the Fully reduced Enzyme with Dioxygen, Biochemistry 36, 13824-13829.
15. Pawate, A. S., Morgan, J., Namslauer, A., Mills, D. A., Brzezinski, P., Ferguson-Miller, S., and Gennis, R. B. (2002) A Mutation in Subunit I of Cytochrome Oxidase from Rhodobacter sphaeroides Results in an Increase in Steady-State Activity but Completely Eliminates Proton Pumping, Biochemistry 41, 13417.
16. Pfitzner, U., Hoffmeier, K., Harrenga, A., Kannt, A., Michel, H., Bamberg, E., Richter, O.-M. H., and Ludwig, B. (2000) Tracing the D-Pathway in Reconstituted Site-Directed Mutants of Cytochrome c Oxidase from Paracoccus denitrificans, Biochemistry 39, 6756-6762.
17. Brzezinski, P., and Larsson, G. (2003) Redox-driven Proton Pumping by Heme-copper Oxidases, Biochim. Biophys. Acta 1605, 1-13.
18. Namslauer, A., Pawate, A., Gennis, R. B., and Brzezinski, P. (2003) Redox-Coupled Proton Translocation in Biological Systems: Proton Shuttling in Cytochrome c Oxidase, Proc. Natl. Acad. Sci. U.S.A. 100, 15543-15547.
19. Mills, D. A., Tan, Z., Ferguson-Miller, S., and Hosler, J. P. (2003) A Role for Subunit III in Proton Uptake into the D Pathway and a Possible Proton Exit Pathway in Rhodobacter sphaeroides Cytochrome c Oxidase, Biochemistry 42, 7410-7417.
20. 2+-NTA Affinity Chromatography, FEBS Lett. 368, 148-150.
21. Riser, C., Mills, D. A., Schall, M., and Ferguson-Miller, S. (2001) C-Terminal Truncation and Histidine-tagging of Cytochrome c Oxidase Subunit II Reveals the Native Processing Site, Shows Involvement of the C-terminus in Cytochrome c Binding, and Improves the Assay for Proton Pumping, Biochemistry 40, 1606-1615.
22. Jasaitis, A., Verkhovsky, M. I., Morgan, J. E., Verkhovskaya, M. L., and Wikström, M. (1999) Assignment and Charge Translocation Stoichiometries of the Major Electrogenic Phases in the Reaction of Cytochrome c Oxidase with Dioxygen, Biochemistry 38, 2697-2706.
23. Rigaud, J.-L., Mosser, G., Lacapere, J.-J., Olofsson, A., Levy, D., and Ranck, J.-L. (1997) Bio-Beads: An Efficient Strategy for Two-Dimensional Crystallization of Membrane Proteins, J. Struct. Biol. 118, 226-235.
24. Verkhovsky, M. I., Morgan, J. E., and Wikström, M. (1995) Control of Electron Delivery to the Oxygen Reduction Site of Cytochrome c Oxidase: A Role for Protons, Biochemistry 34, 7483-7491.
25. Tomson, F. L., Morgan, J. E., Gu, G., Barquera, B., Vygodina, T. V., and Gennis, R. B. (2003) Substitutions for glutamate 101 in subunit II of Cytochrome c oxidase from Rhodobacter sphaeroides result in blocking the proton-conducting K-channel, Biochemistry 42, 1711-1717.
26. 3 of Escherichia coli, Biochemistry 34, 15633-15637.
27. Mills, D. A., and Hosler, J. P. (2005) Slow Proton Transfer Through the Pathways for Pumped Protons in Cytochrome c Oxidase Induces Suicide Inactivation of the Enzyme, Biochemistry 44, 4656-4666.
28. Hosler, J. P. (2004) The Influence of Subunit III of Cytochrome c Oxidase on the D Pathway, the Proton Exit pathway and Mechanism-based Inactivation in Subunit I, Biochim. Biophys. Acta 1655, 332-339.
29. Svensson-Ek, M., Abramson, J., Larsson, G., Tornroth, S., Brzezinski, P., and Iwata, S. (2002) The X-ray Crystal Structures of Wild-type and EQ(I-286) Mutant Cytochrome c Oxidases from Rhodobacter sphaeroides, J. Mol. Biol. 321, 329-339.
30. Smirnova, I. A., Ädelroth, P., Gennis, R. B., and Brzezinski, P. (1999) Aspartate-132 in Cytochrome c Oxidase from Rhodobacter sphaeroides is Involved in a Two-step Proton Transfer during OxoFerryl Formation, Biochemistry 38, 6826-6833.
31. Gilderson, G., Salomonsson, L., Aagaard, A., Gray, J., Brzezinski, P., and Hosler, J. (2003) Subunit III of Cytochrome c Oxidase of Rhodobacter spaheroides is Required to Maintain Rapid Proton Uptake through the D Pathway at Physiologic pH, Biochemistry 42, 7400-7409.
32. Humphrey, W., Dalke, A., and Schulten, K. (1996) VMD: Visual Molecular Dynamics, J. Mol. Graphics 14, 33-38.