Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli

Analytical Biochemistry

Volumn 345, Issue 2, 2005, Pages 336-339

  Guan, Ziqiang ^a   Breazeale, Steven D ^a   Raetz, Christian R H ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

15N mass shift analysis; Escherichia coli; Lipid II; Mass spectrometry

Indexed keywords

ELECTROSPRAY IONIZATION; ESCHERICHIA COLI; MASS SPECTROMETRY;

DIPHOSPHATES; ELECTROSPRAY IONIZATION MASS SPECTROMETRY; ESCHERICHIA COLI CELLS; MASS SHIFT; PH-DEPENDENT SOLUBILITIES; RESISTANT MUTANTS; STRUCTURAL CHARACTERIZATION;

NITROGEN COMPOUNDS;

PEPTIDOGLYCAN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL CELL; BACTERIUM MUTANT; CHEMICAL STRUCTURE; ELECTROSPRAY; ELECTROSPRAY MASS SPECTROMETRY; ESCHERICHIA COLI; EXTRACTION; GENETIC VARIABILITY; LIPID ANALYSIS; NITROGEN NUCLEAR MAGNETIC RESONANCE; NONHUMAN; PRIORITY JOURNAL; SOLUBILITY; STRUCTURE ANALYSIS;

ESCHERICHIA COLI;

EID: 25444485209     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.07.002     Document Type: Article

References (18)

1. K. Lazar, and S. Walker Substrate analogues to study cell-wall biosynthesis and its inhibition Curr. Opin. Chem. Biol. 6 2002 786 793
2. C.T. Walsh Molecular mechanisms that confer antibacterial drug resistance Nature 406 2002 775 781
3. S.T. Hsu, E. Breukink, E. Tischenko, M.A. Lutters, B. de Kruijff, R. Kaptein, A.M. Bonvin, and N.A. van Nuland The nisin-lipid II complex reveals a pyrophosphate cage that provides a blueprint for novel antibiotics Nat. Struct. Mol. Biol. 11 2004 963 967
4. D. Hughes Exploiting genomics, genetics, and chemistry to combat antibiotic resistance Nat. Rev. Genet. 4 2003 432 441
5. 55 isoprenoid alcohol Proc. Natl. Acad. Sci. USA 57 1967 1878 1884
6. 55-isoprenoid alcohol and of lipid intermediates in peptidoglycan synthesis from Staphylococcus aureus J. Biol. Chem. 245 1970 3697 3702
7. J.T. Park, and J.L. Strominger Mode of action of penicillin Science 125 1957 99 101
8. J.T. Park Uridine-5′-pyrophosphate derivatives: II. Isolation from Staphylococcus aureus J. Biol. Chem. 194 1952 877 884
9. J.N. Umbreit, and J.L. Strominger Isolation of the lipid intermediate in peptidoglycan biosynthesis from Escherichia coli J. Bacteriol. 112 1972 1306 1309
10. Y. Van Heijenoort, M. Gómez, M. Derrien, J. Ayala, and J. van Heijenoort Membrane intermediates in the peptidoglycan metabolism of Escherichia coli: possible roles of PBP 1b and PBP 3 J. Bacteriol. 174 1992 3549 3557
11. M.S. Van Nieuwenhze, S.C. Mauldin, M. Zia-Ebrahimi, B.E. Winger, W.J. Hornback, S.L. Saha, J.A. Aikins, and L.C. Blaszczak The first total synthesis of lipid II: the final monomeric intermediate in bacterial cell wall biosynthesis J. Am. Chem. Soc. 124 2002 3656 3660
12. E.G. Bligh, and W.J. Dyer A rapid method of total lipid extraction and purification Can. J. Biochem. Physiol. 37 1959 911 917
13. J.B. Fenn, M. Mann, C.K. Meng, S.F. Wong, and C.M. Whitehouse Electrospray ionization for mass spectrometry of large biomolecules Science 246 1989 64 71
14. J. Sambrook, and D.W. Russell Molecular Cloning: A Laboratory Manual 2001 Cold Spring Harbor Laboratory Press New York
15. K.D. Henry, and F.W. McLafferty Electrospray ionization with Fourier-transform mass spectrometry: charge state assignment from resolved isotopic peaks Org. Mass Spectrom. 25 1990 490 492
16. C.E. Bulawa, and C.R.H. Raetz The biosynthesis of Gram-negative endotoxin: identification and function of UDP-2, 3-diacylglucosamine in Escherichia coli J. Biol. Chem. 259 1984 4846 4851
17. C.R.H. Raetz Molecular genetics of membrane phospholipid synthesis Annu. Rev. Genet. 20 1986 253 295
18. K. Biemann, Incorporation of stable isotopes for structure determination, in: Mass Spectrometry: Organic Chemical Applications, McGraw-Hill, New York, 1962, pp. 231-243.