High-throughput sequencing: A failure mode analysis

BMC Genomics

Volumn 6, Issue , 2005, Pages

  Yang, George S ^a   Stott, Jeffery M ^a   Smailus, Duane ^a   Barber, Sarah A ^a   Balasundaram, Miruna ^a   Marra, Marco A ^a   Holt, Robert A ^a  

^a BRITISH COLUMBIA CANCER RESEARCH CENTRE   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; PLANT DNA; PLASMID DNA;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; AGAR GEL ELECTROPHORESIS; ANALYTIC METHOD; ANALYTICAL ERROR; ARTICLE; ASPEN; COMPUTER PROGRAM; CONTROLLED STUDY; COST CONTROL; DNA LIBRARY; DNA SEQUENCE; DNA TEMPLATE; EXPRESSED SEQUENCE TAG; HIGH THROUGHPUT SCREENING; MATERIALS HANDLING; NONHUMAN; QUALITY CONTROL;

EID: 25444481087     PISSN: 14712164     EISSN: 14712164     Source Type: Journal    
DOI: 10.1186/1471-2164-6-2     Document Type: Article

References (4)

1. Elkin CJ, Richardson PM, Fourcade HM, Hammon NM, Pollard MJ, Predki PF, Glavina T, Hawkins TL: High-throughput plasmid purification for capillary sequencing. Genome Res 2001, 11:1269-1274.
2. Ewing B, Green P: Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res 1998, 8:186-194.
3. Sambrook JRDW: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press; 2001.
4. Gleeson T, Hillier L: A trace display and editing program for data from fluorescence based sequencing machines. Nucleic Acids Res 1991, 19:6481-6483.