Large-scale purification of the proton pumping pyrophosphatase from Thermotoga maritima: A "Hot-Solve" method for isolation of recombinant thermophilic membrane proteins

Biochimica et Biophysica Acta - Biomembranes

Volumn 1716, Issue 1, 2005, Pages 69-76

  López Marqués, Rosa L ^a,b   Pérez Castiñeira, José R ^a   Buch Pedersen, Morten J ^b   Marco, Sergio ^c   Rigaud, Jean Louis ^c   Palmgren, Michael G ^b   Serrano, Aurelio ^a,b  

^a UNIVERSITY OF SEVILLE   (Spain)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c INSTITUT CURIE   (France)

Author keywords

Detergent solubilization; H+ PPase; Ni NTA affinity chromatography; Single particle analysis; Thermotoga maritima

Indexed keywords

INORGANIC PYROPHOSPHATASE; PROTON PUMP;

ARTICLE; CONTROLLED STUDY; ELECTRON MICROSCOPY; GENE OVEREXPRESSION; HETEROLOGOUS EXPRESSION; NONHUMAN; PARTICLE SIZE; PRIORITY JOURNAL; PURIFICATION; SOLUBILITY; STRUCTURE ACTIVITY RELATION; THERMOTOGA MARITIMA; YEAST;

BLOTTING, WESTERN; CELL MEMBRANE; CHROMATOGRAPHY, AFFINITY; DETERGENTS; DIMERIZATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROGEN-ION CONCENTRATION; IMAGE PROCESSING, COMPUTER-ASSISTED; INORGANIC PYROPHOSPHATASE; LIPIDS; MEMBRANE PROTEINS; MICROSCOPY, ELECTRON; MUTAGENESIS; NICKEL; PLASMIDS; PROTONS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; STRUCTURE-ACTIVITY RELATIONSHIP; TEMPERATURE; THERMOTOGA MARITIMA;

THERMOTOGA MARITIMA;

EID: 25444451062     PISSN: 00052736     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbamem.2005.08.004     Document Type: Article

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