Application of phospholipid fatty acid (PLFA) analysis in microbial ecology

Chinese Journal of Ecology

Volumn 24, Issue 7, 2005, Pages 828-832

  Chen, Zhenxiang ^a   Yu, Xin ^a   Xia, Mingfang ^b   Dai, Zhaoxia ^a   Sun, Cheng ^a  

^a NANJING UNIVERSITY   (China)

^b Jiangsu Academy of Environmental Science   (China)

Author keywords

Biomarker; Biomass; Community structure and function; Microbial ecology; Phospholipid fatty acid (PLFA)

Indexed keywords

BIOMARKER; MICROBIAL ECOLOGY; MOLECULAR ANALYSIS; PHOSPHOLIPID;

EID: 25444431710     PISSN: 10004890     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (42)

1. Chinese source. 2000. [J]. 19(6):29-31.
2. Chinese source. 2003. [J]. 23(8):1576-1582.
3. Chinese source. 1999. [J]. 18(4):407-412.
4. Chinese source. 2001. GC/MS, [J]. 2:19-21.
5. Chinese source. 2003. [J]. 17(2):51-54.
6. Albrechtsen HJ, Heron G, Christensen TH. 1995. Limiting factors for microbial Fe(III)-reduction in a landfill leachate polluted aquifer (Vejen, Denmark) [J]. FEMS Microbiol. Ecol. 16:233-247.
7. Bardgett RD, Kandeler E, Tscherko D, et al. 1999. Below-ground microbial community development in a high temperature world[J]. oikos, 85:193-203.
8. Bomeman J, Skroch PW, Palus JA, et al. 1996. Molecular microbial diversity of an agricultural soil in Wisconsin[J]. Appl. Environ. Microbiol., 62(6):1935-1943.
9. Bowman JP, McCammon SA, Gibson JAE, et al. 2003. Metabolic Activity and Community Structure in Antarctic Continental Shelf Sediments[J]. Appl. Environ. Microbiol., 69:2448-2462.
10. Bowman JP, Jimenez L, Rosario I, et al. 1993. Characterization of the methanotrophic bacterial community present in a trichloroethylene-contaminated subsurface groundwater site[J]. Appl.Environ. Microbiol., 59:2380-2387.
11. Cha DK, et al. 1999. Fatty acid methyl ester (FAME) analysis for monitoring Nocardia levels in activated sludge[J]. Wat. Res., 38(8):1964-1966.
12. Christopher T, Green K, Scow M. 2000. Analysis of phospholipid fatty acids (PLFA) to characterize microbial communities in aquifers[J]. Hydrogeology J., 8:126-141.
13. Fang J, Barcelona MJ. 1998. Structural determination and quantitative analysis of bacterial phospholipids using liquid chromatography/ electrospray ionization/mass spectrometry[J]. Microbiol. Methods, 33:23-35.
14. Fredrickson JK, McKinley JP, Bjornstad BN, et al. 1997. Pore-size constraints on the activity and survival of subsurface bacteria in a late Cretaceous shale-sand stone sequence, northwestern New Mexico[J]. Geomicrobiology, 14:183-202.
15. Fritze H, Pietikainen J, Pennanen T. 2000. Distribution of microbial biomass and phospholipid fatty acids in podzol profiles under coniferous forest[J]. Euro. J. Soil Sci., 51:565-573.
16. Frostegard A, Bååth E. 1996. The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil[J]. Biol. Fert. Soils, 22:59-65.
17. Frostegard A, Tunlid A, Baath E. 1991. Microbial biomass measured as total lipid phosphate in soils of different organic content [J]. J. Microbiol. Method, 14:151-163.
18. Frostegard A, Tunlid A, Baath E. 1993. Phospholipid fatty acid composition, biomass and activity of microbial communities from two soil types experimentally exposed to different heavy metals [J]. Appl. Environ. Microbiol., 59:3605-3617.
19. Haack SK, Garchow H, Odelson DA, et al. 1994. Accuracy, reproducibility, and interpretation of fatty acid methyl ester profiles of model bacterial communities[J]. Appl. Environ. Microbiol., 60:2483-2493.
20. Jain DK, Stroes-Gascoyne S, Providenti M, et al. 1997. Characterization of microbial communities in deep groundwater from granitic rock[J]. Can J. Microbiol., 43:272-283.
21. Kandeler E, Tscherko D, Bruce KD, et al. 2000. Structure and function of the soil microbial community in microhabitats of a heavy metal polluted soil[J]. Biol. Fert. Soils, 32:390-400.
22. Kieft TL, Ringelberg DB, White DL. 1994. Changes in esterlinked phospholipid fatty acid profiles of subsurface bacteria during starvation and desiccation in a porous medium[J]. Appl. Environ. Microbiol., 60:3292-3299.
23. Lechevalier MP. 1997. Lipids in bacterial taxonomy A taxonomist's view[J]. Crit Rev. Microbiol., 7:109-210.
24. Liu WT, Marsh TL, Cheng H, et al. 1997. Characterization of Microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA [J]. Appl. Environ. Microbiol., 63(11):4516-4522.
25. Ludvigsen L, Albrechtsen HJ, Ringelberg DB, et al. 1999. Distribution and composition of microbial populations in a landfill leachate contaminated aquifer (Grindsted, Denmark) [J]. Microb. Ecol., 37:197-207.
26. Madigan MT, Martinko JM, Parkerer J. 1999. Brock-Biology of microorganisms. 9th edition [M]. London: Prentice Hall, 53-55.
27. Madsen EL, Sinclair JL, Ghiorse WC. 1991. In situ biodegradation microbiological patterns in a contaminated aquifer[J]. Science, 252:830-833.
28. Makula RA. 1978. Phospholipid composition of methaneutilizing bacteria[J]. J. Bacteriol, 134:771-777.
29. Pennanen T, Frostegard A, Fritze H, et al. 1996. Phospholipid acid composition and heavy metal tolerance of soil microbial communities along two heavy metal-polluted gragients in coniferous forests [J]. Appl. Environ. Microbiol., 62(2):420-428.
30. Perry GJ, Volkman JK, Johns RB, et al. 1979. Fatty acids of bacterial origin contemporary sedimens [J]. Geochim Cosmochim. Acta, 43:1715-1725.
31. Petersen S, Klug M. 1994. Effects of sieving, storage, and incubation temperature on the phospholipid fatty acid profile of a soil microbial community[J]. Appl. Environ. Microbiol., 60: 2421-2430.
32. Priha O, Susan J, et al. 2001. Microbial community structure and characteristics of the organic matter in soils under Pinus sylvestris, Picea abies and Betula pendula at two forest sites [J]. Biol. Fertil. Soils, 33:17-24.
33. Rajendran N, Matsuda O, Imamura N, et al. 1992. Variation in microbial biomass and community in the sediments of eutrophic bays as described by phospholipid ester-linked fatty acids[J]. Appl. Environ. Microbiol., 58:562-571.
34. Ringelberg DB, Sutton S, White DC. 1997. Biomass, bioactivity and biodiversity: microbial ecology of the deep subsurface: An alysis of ester-linked phospholipid fatty acids[J]. FEMS Microbiol. Rev., 20:371-377.
35. Robie J. 1989. Lipid analysis in microbial ecology[J]. BioScience, 39 (8):535-541.
36. Roslev P, Iversen N, Henriksen K. 1998. Direct fingerprinting of metabolically active bacteria in environmental samples by substrate specific radiolabelling and lipid analysis[J]. J. Microbiol. Methods, 31:99-111.
37. Smith CA, Phiefer CB, Macnaughton SJ, et al. 2000. Quantitative lipid biomarker detection of unculturable microbes and chlorine exposure in water distribution system biofilms [J]. Wat. Res., 34 (10):2683-2688.
38. Smith GA, Nickels JS, Kerger BD, et al. 1986. Quantitative characterization of microbial biomass and community structure in subsurface material:A Procaryotic consortium responsive to organic contamination [J]. Can. J. Microbiol., 32:104-111.
39. Tunlid A, Barid BH, Trexler MB, et al. 1985. Determination of phospholipid ester-linked fatty acid and polyß-hydroxybuty rate for the stimulation of bacterial biomass and activity in the rhizosphere of the rape plant B rassicanapus[J]. Can. J. Microbiol., 31:1113-1119.
40. Tunlid A, White DC. 1992. Biochemical analysis of biomass community structure, nutritional status and metabolic activity of microbial communities in soil[J]. Soil Biochem, 7:229-262.
41. Vestal JR, White DC. 1989. Lipid analysis in microbial ecology quantitative approaches to the study of microbial communities [J]. Biosscience, 39:535-541.
42. White DC, Davis WM, Nickels JS, et al. 1979. Determination of the sedimentary microbial biomass by extractable lipid phosphate [J]. Oecologia, 41:51-62.