Relative stability of transgene DNA fragments from GM rapeseed in mixed ruminal cultures

British Journal of Nutrition

Volumn 91, Issue 5, 2004, Pages 673-681

  Sharma, Ranjana ^a   Alexander, Trevor W ^a,b   John, S Jacob ^c   Forster, Robert J ^a   McAllister, Tim A ^a  

^a AGRICULTURE AND AGRI FOOD CANADA   (Canada)

^b UNIVERSITY OF ALBERTA   (Canada)

^c Lethbridge Community College   (Canada)

Author keywords

EPSPS gene fragments; Foreign DNA stability; Genetically modified; Roundup Ready rapeseed; Rubisco

Indexed keywords

3 PHOSPHOSHIKIMATE 1 CARBOXYVINYLTRANSFERASE; AMMONIA; BACTERIAL DNA; DNA FRAGMENT; PLANT DNA; RECOMBINANT DNA; SUGAR PHOSPHATE;

ANIMAL FOOD; ARTICLE; CYTOLYSIS; DIGESTION; DNA DETERMINATION; ENZYME SUBSTRATE; FERMENTATION; GAS; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC STABILITY; GENETICALLY MODIFIED CROP; IN VITRO STUDY; INCUBATION TIME; INTESTINE ABSORPTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT CELL; PLANT SEED; POLYMERASE CHAIN REACTION; RAPESEED; RUMINANT STOMACH; TRANSGENE;

3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE; ALKYL AND ARYL TRANSFERASES; AMMONIA; ANIMALS; BRASSICA RAPA; CATTLE; DIGESTION; DNA FRAGMENTATION; DNA, BACTERIAL; DNA, PLANT; DNA, RECOMBINANT; MALE; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; RIBULOSE-BISPHOSPHATE CARBOXYLASE; RUMEN; TRANSGENES;

ANIMALIA; BACTERIA (MICROORGANISMS); HORDEUM VULGARE SUBSP. VULGARE; SINAPIS ARVENSIS;

EID: 2542573232     PISSN: 00071145     EISSN: None     Source Type: Journal    
DOI: 10.1079/BJN20041100     Document Type: Article

References (27)

1. Alexander TW, Sharma R, Okine EK, Dixon WT, Forster RJ, Stanford K & McAllister TA (2002) Impact of feed processing and mixed ruminal culture on the fate of recombinant EPSP synthase and endogenous canola plant DNA. FEMS Microbiol Lett 214, 263-269.
2. Broderick GA & Kang JH (1980) Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J Dairy Sci 63, 64-75.
3. Canola Council of Canada (2001), Impact of Transgenic Canola on Growers, Industry and Environment. www.canola-council. org/manual/GMO/gmo_main.htm
4. Cheng K-JJ & McAllister TA (1997) Compartmentation in the rumen. In The Rumen Microbial Ecosystem, 2nd ed. pp. 492-522 [PN Hobson and CS Stewart, editors]. London: Blackie Academic and Professional.
5. Duggan PS, Chambers PA, Heritage J & Forbes JM (2000) Survival of free DNA encoding antibiotic resistance from transgenic maize and the transformation activity of DNA in ovine saliva, ovine rumen fluid and silage effluent. FEMS Microbiol Lett 191, 71-77.
6. Duggan PS, Chambers PA, Heritage J & Forbes JM (2003) Fate of genetically modified maize DNA in the oral cavity and rumen of sheep. Br J Nutr 89, 159-166.
7. Einspanier R, Klotz A, Kraft J, Aulrich K, Poser R, Schwagele F, Jahreis G & Flachowsky G (2001) The fate of forage plant DNA in farm animals: a collaborative case-study investigating cattle and chicken fed recombinant plant material. Eur Food Res Technol 212, 129-134.
8. Fedorak PM & Hrudey SE (1983) A simple apparatus for measuring gas production by methanogenic cultures in serum bottles. Environ Technol Lett 4, 425-432.
9. Garcia-Vallve S, Romeu A & Palau J (2000) Horizontal gene transfer of glycosyl hydrolases of the ramen fungi. Mol Biol Evol 17, 352-361.
10. Health Canada (1999) Novel Food Information - Food Biotechnology. Glyphosate Tolerant Canola, GT200. http://www.hc-sc.gc.ca/food-aliment/mh-dm/ofb-bba/ nfi-ani/e_ofb-097-325-a.html
11. Hohlweg U & Doerfler W (2001) On the fate of plant or other foreign genes upon the uptake in food or after intramuscular injection in mice. Mol Genet Genomics 265, 225-233.
12. Kleter GA & Kuiper HA (2002) Considerations for the assessment of the safety of genetically modified animals used for human food or animal feed. Livest Prod Sci 74, 275-285.
13. McAllan AB & Smith RH (1973) Degradation of nucleic acids in the rumen. Br J Nutr 29, 331-345.
14. McAllister TA, Bae HD, Jones GA & Cheng K-J (1994) Microbial attachment and feed digestion in the rumen. J Anim Sci 72, 3004-3018.
15. Martens MA (2000) Safety evaluation of genetically modified foods. Int Arch Occup Environ Health 73, Suppl. 1, S14-S18.
16. Menke KH, Raab L, Salewski A, Steinglass H, Fritz D & Schneider W (1979) The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with ruminal culture on the fate of recombinant EPSP synthase and endogenous canola plant DNA. FEMS Microbiol Lett 214, 222-263.
17. Morrison M (1996) Do ruminal bacteria exchange genetic material? J Dairy Sci 79, 1476-1486.
18. Nadkarni MA, Martin FE, Jacques NA & Hunter N (2002) Determination of bacterial load by real-time PCR using a broad range (universal) probe and primer set. Microbiol SGM 148, 257-266.
19. Nikolich MP, Hong G, Shoemaker NB & Salyers AA (1994) Evidence for natural horizontal transfer of tetQ between bacteria that normally colonize humans and bacteria that normally colonize livestock. Appl Environ Microbiol 60, 3255-3260.
20. Organization of Economic Cooperation and Development (1993) Food safety and biotechnology: concepts and principles. In Safety Evaluation of Foods Derived by Modern Biotechnology: Concepts and Principles, pp. 10-13 [Environment Directorate, OECD, editors]. Paris: OECD, http://www.oecd.org/dataoecd/57/3/1946129.pdf.
21. Phipps RH, Beever DE & Humphries DJ. (2002) Detection of transgenic DNA in milk from cows receiving herbicide tolerant (CP4EPSPS) soyabean meal. Livest Prod Sci 74, 269-273.
22. Ruiz TR, Andres S & Smith GB (2000) Identification and characterization of nuclease activities in anaerobic environmental samples. Can J Microbiol 46, 736-740.
23. Sambrook J, Fritsch EF & Maniatis T (1989) Gel electrophoresis of DNA. In Molecular Cloning: A Laboratory Manual, 2nd ed. pp. 6.9-6.13 [N Ford and C Nolan, editors]. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory 1.
24. Smith RH & McAllan AB (1970) Nucleic acid metabolism in the ruminant. Br J Nutr 24, 545-556.
25. Statistics Canada (2003), Livestock Feed Requirements Study. Canada and Provinces 1999, 2000, 2001, Catalogue no. 23-501-XIE. Ottawa, Canada: Statistics Canada.
26. Wallace RJ, Onodera R & Cotta MA (1997) Metabolism of nitrogen-containing compounds. In The Rumen Microbial Ecosystem, pp. 283-328 [PN Hobson and CS Stewart, editors]. New York: Blackie Academic & Professional.
27. Wang Y, McAllister TA, Zobell DR, Pickard MD, Rode LM, Mir Z & Cheng K-J (1997) The effect of micronization of full-fat canola seed on digestion in the rumen and total tract of dairy cows. Can J Anim Sci 77, 431-440.