Molecular characterization of a stably transformed Bombyx mori cell line: Identification of alternative transcriptional initiation sites of the A3 cytoplasmic actin gene

Molecular and General Genetics

Volumn 260, Issue 1, 1998, Pages 1-8

  Fatyol, K ^a   Illes, K ^a   Praznovszky, T ^a,c   Langridge, W H R ^a,b   Hadlaczky, G ^a,c   Szalay, A A ^a,b  

^a LOMA LINDA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b Dept Microbiol and Molec Genet ^*

^c INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

5' RACE; A3 cytoplasmic actin; Puromycin N acetyl transferase; Silkworm; Stably transformed cell line

Indexed keywords

ACTIN; ACYLTRANSFERASE; MESSENGER RNA; PUROMYCIN;

ANIMAL CELL; ARTICLE; CELL LINE; CELL TRANSFORMATION; CONTROLLED STUDY; IN SITU HYBRIDIZATION; INTRON; MARKER GENE; NONHUMAN; PLASMID; PRIORITY JOURNAL; SILKWORM; SOUTHERN BLOTTING; START CODON; TRANSCRIPTION INITIATION;

ACETYLTRANSFERASES; ACTINS; ANIMALS; BASE SEQUENCE; BOMBYX; CELL LINE, TRANSFORMED; GENETIC MARKERS; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT FUSION PROTEINS; REGULATORY SEQUENCES, NUCLEIC ACID; SELECTION (GENETICS); TRANSCRIPTION, GENETIC; TRANSFECTION;

BOMBYX; BOMBYX MORI; INSECTA;

EID: 2542549807     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380050864     Document Type: Article

References (19)

1. Angelichio ML, Beck JA, Johansen H, Ivey-Hoyle M (1991) Comparison of several promoters and polyadenylation signals for use in heterologous gene expression in cultured Drosophila cells. Nucleic Acids Res 19:5037-5043
2. Ayoubi TAY, Van de Ven WJM (1996) Regulation of gene expression by alternative promoters. FASEB J 10:453-160
3. Chen C, Okayama H (1987) High-efficiency transformation of mammalian cells by plasmid DNA. Mol Cell Biol 7:2745-2752
4. Chung YT, Keller EB (1990a) Regulatory elements mediating transcription from the Drosophila melanogaster actin 5C proximal promoter. Mol Cell Biol 10:206-216
5. Chung YT, Keller EB (1990b) Positive and negative regulatory elements mediating transcription from the Dorsophila melanogaster actin 5C distal promoter. Mol Cell Biol 10:6172-6180
6. Hui C-c, Matsuno K, Suzuki Y (1990) Fibroin gene promoter contains a cluster of homeodomain binding sites that interact with three silk gland factors. J Mol Biol 213:651-670
7. Johnson R, Meidinger RG, Iatrou K (1992) A cellular promoter based expression cassette for generating recombinant baculoviruses directing rapid expression of passenger genes in infected insects. Virology 190:815-823
8. Lu M. Farrell PJ, Johnson R, Iatrou K (1997) A baculovirus (Bombyx mori nuclear polyhedrosis virus) repeat element functions as a powerful constitutive enhancer in transfected insect cells. J Biol Chem 272:30724-30728
9. Mange A, Couble P, Prudhomme JC (1996) Two alternative promoters drive the expression of the cytoplasmic actin A4 gene of Bombyx mori. Gene 183:1919-199
10. Mange A, Julien, E, Prudhomme JC, Couble P (1997) A strong inhibitory element down-regulates SRE-stimulaled transcription of the A3 cytoplasmic actin gene of Bombyx mori. J Mol Biol 265:266-274
11. McLachlin JR, Miller (1997) Stable transformation of insect cells to coexpress a rapidly selectable marker gene and an inhibitor of apoptosis. In Vitro Cell Dev Biol. Animal 33:575-579
12. Mounier N, Prudhomme JC (1986) Isolation of actin genes in Bombyx mori: the coding sequence of a cytoplasmic actin gene expressed in the silkgland is interrupted by a single intron in an unusual position. Biochimie 68:1053-1061
13. Mounier N, Prudhomme JC (1991) Differential expression of muscle and cytoplasmic actin genes during development of Bombyx mori. Insect Biochem 21:523-533
14. Mounier N, Coulon M, Prudhomme JC (1991) Expression of a cytoplasmic actin gene in relation to the silk production cycle in the silk glands of Bombyx mori. Insect Bioehem 21:293-301
15. Mounier N, Gouy M, Mouchiroud D, Prudhomme JC (1992) Insect muscle actins differ distinctly from invertebrate cytoplasmic actins. J Mol Evol 34:406-415
16. Morgenstem JP, Land H (1990) Advanced mammalian gene transfer: high-titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. Nucleic Acids Res 18:3587-3596
17. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual (2nd edn). Cold Spring Harbor Laboratory Press, Cold Schibler Harbor, New York
18. Schibler U, Sierra F (1987) Alternative promoters in developmental gene expression. Ann Rev Genet 21:237-257
19. Wiegant J, Ried T, Nederlof PM, van der Ploeg M, Tanke HJ, Raap AK (1991) In situ hybridization with fluoresceinated DNA. Nucleic Acids Res 19:3237-3241