Electron conduction across electrode-immobilized neutravidin bound with biotin-labeled ruthenium pentaamine

Journal of the American Chemical Society

Volumn 126, Issue 21, 2004, Pages 6540-6541

  Jhaveri, Sulay D ^a   Trammell, Scott A ^b   Lowy, Daniel A ^a   Tender, Leonard M ^b  

^a NOVA RESEARCH INC   (United States)

^b NAVAL RESEARCH LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AVIDIN; BIOTIN DERIVATIVE; GOLD; NEUTRAVIDIN; RUTHENIUM DERIVATIVE; RUTHENIUM PENTAAMINE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL STRUCTURE; CYCLIC POTENTIOMETRY; ELECTRICITY; ELECTRODE; ELECTRON; ELECTRON TRANSPORT; ENZYME IMMOBILIZATION; OXIDATION REDUCTION REACTION; REACTION ANALYSIS; TECHNOLOGY;

AMINES; AVIDIN; BIOTIN; ELECTROCHEMISTRY; ELECTRODES; ELECTRONS; MODELS, MOLECULAR; MOLECULAR STRUCTURE; ORGANOMETALLIC COMPOUNDS; PROPIONIC ACIDS; RUTHENIUM; STAINING AND LABELING;

EID: 2542515320     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja037949g     Document Type: Article

References (25)

1. Eddowes, M. J.; Hill, H. O. A. J. Chem. Soc., Chem. Comm. 1977, 45, 771.
2. Yeh, P.; Kuwana, T. Chem. Lett. 1997, 10, 1145-1148.
3. El Kasmi, A.; Wallace, J. M.; Bowden, E. F.; Binet, S. M.; Linderman, R. J. J. Am. Chem. Soc. 1998, 120, 225-226.
4. Mano, N.; Mao, F.; Heller, A. J. Am. Chem. Soc. 2003, 125, 6588-6594.
5. Heller, A. Acc. Chem. Res. 1990, 23, 128-134.
6. Xiao, Y.; Patolsky, F.; Katz, E.; Hainfeld, J. F.; Willner, I. Science 2003, 299, 1877-1881.
7. Willner, I.; Riklin, A. Anal. Chem. 1994, 66, 1535-1539.
8. Hess, C. R.; Juda, G. A.; Dooley, D. M.; Amii, R. N.; Hill, M. G.; Winkler, J. R.; Gray, H. B. J. Am. Chem. Soc. 2003, 125, 7156-7157.
9. Anicet, N.; Anne, A.; Moiroux, J.; Saveant, J.-M. J. Am. Chem. Soc. 1998, 120, 7115-7116.
10. Tanaka, S.; Yamamoto, F.; Sugarwara, K.; Nakumura, H. Talanta 1997 44, 357-363.
11. Benson, D. E.; Conrad, D. W.; de Lorimer, R. M.; Trammell, S. A.; Hellinga, H. W. Science 2001, 293, 1641-1644.
12. Collison, M.; Bowden, E. F.; Tarlov, M. J. Langmuir 1992, 8, 1247-1992.
13. Avila, A.; Gregory, B. W.; Katsumi, N.; Cotton, T. M. J. Phys. Chem. B. 2000, 104, 2759-2766.
14. Pugliese, L.; Coda, A.; Malcovati, M.; Bolognesi, M. J. Mol. Biol. 1993, 231, 698-710.
15. Jhaveri, S. D.; Mauro, J. M.; Goldston, H. M.; Schauer, C. L.; Tender, L. M.; Trammell, S. A. Chem. Commun. 2003, 3, 338-339.
16. Sun, L.; Johnson, B.; Wade, T.; Crooks, R. M. J. Phys. Chem. 1990, 94, 8869-871.
17. 4 (31.6%) to zero groups.
18. Labeling efficiency confirmed by UV-vis spectroscopy.
19. Leggett, G. L.; Roberts, C. J.; Williams, P. M.; Davies, M. C.; Jackson, D. E.; Tendler, S. J. B. Langmuir 1993, 9, 2356-2362.
20. EDC activation of a carboxylic acid forms a short-lived positively charged ester intermediate that reverts back to carboxylic acid if unreacted. Hermanson, G. T. Bioconjugate Techniques; Academic Press: San Diego, CA, 1996; p 171.
21. Gray, H. B.; Winkler, J. R. Annu. Rev. Biochem. 1996, 65, 537-561.
22. All voltammetry depicted was initiated at a negative voltage limit. Anodic peak current was utilized for determination of apparent electrode coverage due to possible contribution of oxygen reduction to cathodic peak current. Peak integration was based on straightforward subtraction of background current approximated by the linear baseline, which is imprecise due to long-drawn out anodic current that persists after reversal of scan at a positive voltage limit (presumably due to heterogeneity among orientation of immobilized conjugates); this has the effect of making the anodic peak appear larger than the cathodic peak. We have observed the same effect in revisiting our earlier published results (ref 15) and those of Bowden et al. (ref 12).
23. Chidsey, C. E. D. Science 1991, 251, 919.
24. Jeuken, L. J. C. Biochem. Biophys. Acta 2003, 1604, 67-76.
25. Trammell, S. A.; Goldston, H. M.; Tran, P. T.; Tender, L. M.; Conrad, D. W.; Benson, D. E.; Hellinga, H. W. Bioconjugate Chem. 2001, 12, 643-647.