Purification and characterization of an endo-1, 4-β-D glucanase from the cellulolylic system of the wood-boring marine mollusk Lyrodus pedicellatus (Bivalvia: Teredinidae)

Marine Biology

Volumn 144, Issue 5, 2004, Pages 947-953

  Xu, Pei Ning ^a,b   Distel, D L ^a  

^a UNIVERSITY OF MAINE   (United States)

^b GPC Biotech Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME;

BIVALVIA; LYRODUS; LYRODUS PEDICELLATUS; MOLLUSCA; TEREDINIBACTER TURNERAE; TEREDINIDAE; VISCUM; VISCUM ALBUM;

EID: 2542454963     PISSN: 00253162     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00227-003-1251-0     Document Type: Article

References (35)

1. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
2. Breznak JA, Brune A (1994) Role of microorganisms in the digestion of lignocellulose by termites. Annu Rev Entomol 39:453-487
3. Coughlan MP, Mayer F (1992) The cellulose-decomposing bacteria and their enzyme systems. In: Ballows A, Truper H, Harder W, Schleifer KH (eds) Springer, Berlin Heidelberg New York, pp 451-516
4. Dean R (1978) Mechanisms of wood digestion in the shipworm Bankia gouldi Bartsch: enzyme degradation of celluloses, hemicelluloses, and wood cell walls. Biol Bull (Woods Hole) 155:297-316
5. Distel DL (2003) The biology of wood-boring bivalves and their bacterial endosymbionts. In: Schulz TP, Goodell B, Nicholas DD (eds) Wood deterioration and preservation: advances in our changing world. American Chemical Society, Washington, D.C., pp 253-271
6. Distel DL, Delong EF, Waterbury JB (1991) Phylogenetic characterization and in situ localization of the bacterial symbiont of shipworms (Teredinidae: Bivalvia) by using 16S rRNA sequence analysis and oligonucleotide probe hybridization. Appl Environ Microbiol 57:2376-2382
7. Distel DL, Beaudoin DJ, Morrill W (2002a) Coexistence of multiple proteobacterial endosymbionts in the gills of the wood-boring bivalve Lyrodus pedicellatus (Bivalvia: Teredinidae). Appl Environ Microbiol 68:6292-6299
8. Distel DL, Morrill W, MacLaren-Toussaint N, Franks D, Waterbury J (2002b) Teredinibacter turnerae gen. nov., sp. nov., a dinitrogen-fixing, cellulolytic, endosymbiotic gamma-proteobacterium isolated from the gills of wood-boring molluscs (Bivalvia: Teredinidae). Int J Syst Evol Microbiol 52:2261-2269
9. Fauth U, Romaniec MPM, Kobayashi T, Demain AL (1991) Purification and characterization of endoglucanase Ss from Clostridium thermocellum. Biochem J 279:67-73
10. Gallager SM, Turner RD, Berg CJ (1981) Physiological aspects of wood consumption, growth, and reproduction in the shipworm Lyrodus pedicellatus Quatrefages. J Exp Mar Biol Ecol 52:63-77
11. Greene RV (1994) Challenges from the sea: marine shipworms and their symbiotic bacterium. Soc Indust Microbiol News 44:51-59
12. Greene RV, Griffin HL, Freer SN (1988) Purification and characterization of an extracellular endoglucanase from the marine shipworm bacterium. Arch Biochem Biophys 267:334-341
13. Greenfield LJ (1959) Cellulytic activity in teredo. In: Ray DL (ed) Marine boring and fouling organisms. University of Washington Press, Seattle, pp 363-371
14. Griffin HL, Freer SN, Greene RV (1987) Extracellular endoglucanase activity by a novel bacterium isolated from marine shipworm. Biochem Biophys Res Commun 144:143-151
15. Imam SH, Greene RV, Hockridge ME (1993) Zymographic analyses of carboxymethylcellulases secreted by the bacterium from wood-boring marine shipworms. Biotechnol Tech 7:579-584
16. Kane MD (1996) Microbial fermentation in insect guts. In: Mackie RI, White BA (eds) Gastrointestinal fermentation and ecosystems. Chapman and Hall, New York, p 38
17. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
18. Liu DL, Walden CC (1970) Enzymes of glucose metabolism in the caecum of the marine borer Bankia setacea. J Fish Res Board Can 27:1141-1146
19. Miller GL, Blum R, Glennon WE, Burton AL (1960) Measurement of carboxymethyl cellulase activity. Anal Biochem 2:127-132
20. Nakashima K, Watanabe H, Saitoh H, Tokuda G, Azuma JI (2002) Dual cellulose-digesting system of the wood-feeding termite, Coptotermes formosanus Shiraki. Insect Biochem Mol Biol 32:777-784
21. Perito B, Hanhart E, Irdani T, Iqbal M, McCarthy AJ, Mastromei G (1994) Characterization and sequence analysis of a Streptomyces rochei A2 endoglucanase-encoding gene. Gene 148:119-124
22. Popham JD (1974) Further observations of the gland of Deshayes in the teredo Bankia australis. Veliger 18:55-59
23. Popham JD, Dickson MR (1973) Bacterial associations in the teredo Bankia australis (Lamellibranchia, Mollusca). Mar Biol 19:338-340
24. Smant G, Stokkermans JP, Yan Y, de Boer JM, Baum TJ, Wang X, Hussey RS, Gommers FJ, Henrissat B, Davis EL, Helder J, Schots A, Bakker J (1998) Endogenous cellulases in animals: isolation of beta-1, 4-endoglucanase genes from two species of plant-parasitic cyst nematodes. Proc Natl Acad Sci USA 95:4906-4911
25. Suzuki K, Ojima T, Nishita K (2003) Purification and cDNA cloning of a cellulase from abalone Haliotis discus Hannai. Eur J Biochem 270:771-778
26. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350-4354
27. Turner RD (1966) A survey and illustrated catalogue of the Teredinidae (Mollusca: Bivalvia). The Museum of Comparative Zoology, Harvard University, Cambridge
28. Watanabe H, Tokuda G (2001) Animal cellulases. Cell Mol Life Sci 58:1167-1178
29. Watanabe H, Hiroaki N, Tokada G, Lo N (1998) A cellulase gene of termite origin. Nature 394:330-331
30. Waterbury JB, Calloway CB, Turner RD (1983) A cellulolytic-nitrogen fixing bacterium cultured from the gland of Deshayes in shipworms (Bivalvia: Teredinidae). Science 221:1401-1403
31. Wood TM (1988) Preparation of crystalline, amorphous and dyed cellulase substrates. In: Wood WA, Kellogg ST (eds) Biomass, part A: cellulose and hemicellulose. Academic, New York, pp 19-25
32. Wood TM, Bhat KM (1988) Methods for measuring cellulase activities. In: Wood WA, Kellogg ST (eds) Biomass, part A: cellulose and hemicellulose. Academic, New York, pp 87-112
33. Xu B, Hellman U, Ersson B, Janson JC (2000) Purification, characterization and amino-acid sequence analysis of a thermostable, low molecular mass endo-beta-1,4-glucanase from blue mussel, Mytilus edulis. Eur J Biochem 267:4970-4977
34. Xu B, Janson JC, Sellos D (2001) Cloning and sequencing of a molluscan endo-beta-1,4-glucanase gene from the blue mussel, Mytilus edulis. Eur J Biochem 268:3718-3727
35. Yan Y, Smant G, Stokkermans J, Qin L, Helder J, Baum T, Schots A, Davis E (1998) Genomic organization of four beta-1,4-endoglucanase genes in plant-parasitic cyst nematodes and its evolutionary implications. Gene 220:61-70