Escherichia coli methionine aminopeptidase with Tyr168 to alanine substitution can improve the N-terminal processing of recombinant proteins with valine at the penultimate position

Analytical Biochemistry

Volumn 329, Issue 2, 2004, Pages 345-347

  Liu, Li Fan ^a   Hsieh, Cheng Hsilin ^a   Liu, Pei Fen ^a   Tsai, Shu Ping ^a   Tam, Ming F ^a  

^a INSTITUTE OF MOLECULAR BIOLOGY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI; RECOMBINANT PROTEINS;

ALANINE SUBSTITUTION; METHIONINE AMINOPEPTIDASE; N-TERMINALS;

AMINO ACIDS;

ALANINE; METHIONYL AMINOPEPTIDASE; RECOMBINANT PROTEIN; TYROSINE DERIVATIVE; VALINE;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; MOLECULAR MECHANICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION; REACTION ANALYSIS; REACTION OPTIMIZATION; STRUCTURE ANALYSIS; SUBSTITUTION REACTION; WILD TYPE;

ESCHERICHIA COLI;

EID: 2542417350     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.03.031     Document Type: Article

References (6)

1. Hwang D.D., Liu L.F., Kuan I.C., Lin L.Y., Tam T.C., Tam M.F. Co-expression of glutathione S-transferase with methionine aminopeptidase: a system of producing enriched N-terminal processed proteins in Escherichia coli. Biochem. J. 338:1999;335-342
2. Lowther W., Matthews B. Structure and function of the methionine aminopeptidases. Biochim. Biophys. Acta. 1477:2000;157-167
3. Liu L.F., Hong J.L., Tsai S.P., Hsieh J.C., Tam M.F. Reversible modification of rat liver glutathione S-transferase 3-3 with 1-chloro-2, 4-dinitrobenzene: specific labelling of Tyr-115. Biochem. J. 296:1993;189-197
4. Lowther W., Orville A., Madden D., Lim S., Rich D., Matthews B. Escherichia coli methionine aminopeptidase: implications of crystallographic analyses of the native, mutant, and inhibited enzymes for the mechanism of catalysis. Biochemistry. 38:1999;7678-7688
5. Walker K.W., Bradshaw R.A. Yeast methionine aminopeptidase I. Alternation of substrate specificity by site-directed mutagenesis. J. Biol. Chem. 274:1999;13403-13409
6. Shen T.J., Ho N.T., Zou M., Sun D.P., Cottam P.F., Simplaceanu V., Tam M.F., Bell D.A., Ho C. Production of human normal adult and fetal hemoglobins in Escherichia coli. Protein Eng. 10:1997;1085-1097