Presence of two different active nirS nitrite reductase genes in a denitrifying Thauera sp. from a high-nitrate-removal-rate reactor

Applied and Environmental Microbiology

Volumn 71, Issue 9, 2005, Pages 5642-5645

  Etchebehere, Claudia ^a,c   Tiedje, James ^b  

^a UNIVERSIDAD DE LA REPÚBLICA   (Uruguay)

^b MICHIGAN STATE UNIVERSITY   (United States)

^c UNIVERSIDAD DE LA REPÚBLICA   (Uruguay)

Author keywords

[No Author keywords available]

Indexed keywords

DENITRIFICATION; NITRATES; RNA;

DENITRIFYING REACTORS; NITRITE REDUCTASE GENES; STRAIN 28;

GENES;

NITRATE; NITRITE REDUCTASE; RNA 16S;

ENZYME;

ALCALIGENES FAECALIS; ARTICLE; AZOARCUS; BACTERIAL GENE; BACTERIAL STRAIN; DENITRIFICATION; GENE CONTROL; GENE EXPRESSION; GENE SEQUENCE; MARKER GENE; NIRS GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PSEUDOMONAS STUTZERI; REACTOR; RNA GENE; SPECIES DIFFERENCE; THAUERA; THAUERA MECHERNICHENSIS;

BIOREACTORS; DNA, BACTERIAL; GENE EXPRESSION REGULATION, BACTERIAL; MOLECULAR SEQUENCE DATA; NITRATES; NITRITE REDUCTASES; PHYLOGENY; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; THAUERA;

ALCALIGENES FAECALIS; AZOARCUS; PSEUDOMONAS; THAUERA; THAUERA MECHERNICHENSIS;

EID: 25144518331     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.9.5642-5645.2005     Document Type: Article

References (13)

1. 2O release and on the community structure of ammonia oxidizers and denitrifiers. Appl. Environ. Microbiol. 68:5685-5692.
2. Braker, G., H. Ayala del Rio, A. H. Devol, A. Fesefeldt, and J. M. Tiedje. 2001. Community structure of denitrifiers, Bacteria, and Archaea along redox gradients in Pacific Northwest marine sediments by terminal restriction fragment length polymorphism analysis of amplified nitrite reductase (nirS) and 16S rRNA genes. Appl. Environ. Microbiol. 67:1893-1901.
3. Braker, G., A. Fesefeldt, and K.-P. Witzel. 1998. Development of PCR primer systems for amplification of nitrite reductase genes (nirK and nirS) to detect denitrifying bacteria in environmental samples. Appl. Environ. Microbiol. 64:3769-3775.
4. Dayhoff, M. O. 1972. Atlas of protein sequence and structure. National Biomedical Research Foundation, Washington, D.C.
5. Etchebehere, C., A. Cabezas, P. Dabert, and L. Muxí. 2003. Evolution of the bacterial community during granules formation in denitrifying reactors followed by molecular, culture-independent techniques. Water Sci. Technol. 48(6):75-79.
6. Etchebehere, C., M. I. Errazquin, A. Cabezas, M. J. Pianzzola, M. Mallo, G. Ottonello, L. Borzacconi, and L. Muxí. 2002. Sludge bed development in denitrifying reactors using different inocula-performance and microbiological aspects. Water Sci. Technol. 45(10):365-370.
7. Felsenstein, J. 1997. PHYLIP phytogeny inference package (version 3.2). Department of Genetics, University of Washington, Seattle.
8. Knowles, R. 1982. Denitrification. Microbiol. Rev. 46:43-70.
9. Philippot, L. 2002. Denitrifying genes in bacterial and archaeal genomes. Biochim. Biophys. Acta 1577:355-376.
10. Saitou, N., and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. J. Mol. Evol. 4:406-425.
11. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
12. Song, B., and B. B. Ward. 2003. Nitrite reductase genes in halobenzoate degrading denitrifying bacteria. FEMS Microbiol. Ecol. 43:349-357.
13. Zumft, W. G. 1992. The denitrifying prokaryotes, p. 554-582. In A. Balows, H. G. Truper, M. Dworkin, W. Harder, and K.-H. Schleifer (ed.), The prokaryotes. Springer Verlag, New York, N.Y.