A new video image analysis system to study red blood cell dynamics and oxygenation in capillary networks

Microcirculation

Volumn 12, Issue 6, 2005, Pages 489-506

  Japee, Shruti A ^a   Pittman, Roland N ^a   Ellis, Christopher G ^b  

^a Virginia Commonwealth University   (United States)

^b UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

Microcirculation; Oxygen saturation; Red blood cell dynamics

Indexed keywords

HEMOGLOBIN; OXYGEN;

ACCURACY; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; AUDIOVISUAL EQUIPMENT; BLOOD FLOW VELOCITY; BLOOD OXYGEN TENSION; BLOOD VESSEL DIAMETER; CALCULATION; CAPILLARY DENSITY; CAPILLARY FLOW; CONTROLLED STUDY; ERYTHROCYTE; GEOMETRY; HAMSTER; HEMODYNAMICS; IMAGE ANALYSIS; IMAGE DISPLAY; IMAGE PROCESSING; LIGHT INTENSITY; MATHEMATICAL COMPUTING; MEASUREMENT; MICROCIRCULATION; MICROSCOPY; MORPHOLOGY; NONHUMAN; OPTICAL DENSITY; OXYGEN TRANSPORT; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RAT; SIGNAL NOISE RATIO; SPECTRAL SENSITIVITY; TISSUE OXYGENATION; VALIDATION PROCESS; VIDEORECORDING;

ANIMALS; CAPILLARIES; CRICETINAE; ERYTHROCYTES; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY, VIDEO; MUSCLE, SKELETAL; OXYGEN CONSUMPTION; RATS;

EID: 25144452018     PISSN: 10739688     EISSN: None     Source Type: Journal    
DOI: 10.1080/10739680591003332     Document Type: Article

References (30)

1. Brunner J, Krummenauer F, Lehr HA. (2000). Quantification of video-taped images in microcirculation research using inexpensive imaging software (Adobe Photoshop). Microcirculation 7:103-107.
2. Burgess W, Sibbald R, Ellis CG, Groom AC. (1995). Two-dimensional variance maps of network morphology. Microcirculation 2:75.
3. Burkell JA, Ellis CG. (1996). Imaging of the functional capillary bed in skeletal muscle. Ann Biomed Eng 24:1
4. Burkell JA, Neary J, Ellis CG. (1998). Combining two image types into a single image of the microvasculature. J Vasc Res 35:2.
5. Dong M. (1997). Influence of Aging on Oxygen Transport in the Microcirculation of Skeletal Muscle. Thesis, Department of Physiology, Virginia Commonwealth University, Richmond.
6. Ellis CG, Ellsworth ML, Pittman RN. (1990). Determination of red blood cell oxygenation in vivo by dual video densitometric image analysis. Am J Physiol 258:H1216-H1223.
7. Ellis CG, Ellsworth ML, Pittman RN, Burgess WL. (1992). Application of image analysis for evaluation of red blood cell dynamics in capillaries. Microvasc Res 44:214-225.
8. Ellis CG, Fraser S, Hamilton G, Groom AC. (1984). Measurement of the lineal densitv of red blood cells in capillaries in vivo, using a computerized frame-by-frame analysis of video images. Microvasc Res 27:1-13.
9. Ellis CG, Tyml K, Burgess W. (1987). Quantification of red cell movement in microvessels: a new application of interactive computer graphics. Microvasc Res 33:428-432.
10. Ellis CG, Wrigley SM, Potter RF, Groom AC. (1990). Temporal distributions of red cell supply rate to individual capillaries of resting skeletal muscle, in frog and rat. Int J Microcirc Clin Exp 9:67-84.
11. Ellsworth ML, Pittman RN, Ellis CG. (1987). Measurement of hemoglobin oxygen saturation in capillaries. Am J Physiol 252:H1031-H1040.
12. Ellsworth ML, Popel AS, Pittman RN. (1988). Assessment and impact of heterogeneities of convective oxygen transport parameters in capillaries of striated muscle: experimental and theoretical. Microvasc Res 35:341-362.
13. Howling DH, Fitzgerald PJ. (1959). The nature, significance, and evaluation of the Schwarzschild-Villiger (SV) effect in photometric procedures. J Biophys Biochem Cytol 6:313-337.
14. Inoue, S. (1986). Video Microscopy. New York: Plenum.
15. Jain AK. (1989). Fundamentals of Digital Image Processing. Englewood Cliffs, NJ: Prentice Hall.
16. Japee SA, Ellis CG, Pittman RN. (2004). Flow visualization tools for image analysis of capillary networks. Microcirculation 11:39-54.
17. Japee SA, Pittman RN, Ellis CG. (2004). Automated method for tracking individual red blood cells within capillaries to compute velocity and oxygen saturation. Microcirculation 12:507-515.
18. Johnson PC. (1986). Flow measurement techniques in the microcirculation. In Microcirculatory Technology (WL Nastuk, Ed.). Orlando, FL: Academic Press, 149-160.
19. Klyscz T, Junger M, Jung F, Zeintl H. (1997). Cap image-a new kind of computer-assisted video image analysis system for dynamic capillary microscopy. Biomed Tech (Berl) 42:168-175.
20. Piller H. (1977). Systematic measuring errors. In Microscope Photometry. Berlin: Springer-Verlag, 172-179.
21. Pittman R. (1986). Microvessel blood oxygen measurement techniques. In Microcirculatory Technology (WL Nastuk, Ed.). Orlando, FL: Academic Press, 367-389.
22. Popel AS, Greene AS, Ellis CG, Ley KF, Skalak TC, Tonellato PJ. (1998). The Microcirculation Physiome Project. Ann Biomed Eng 26:911-913.
23. Pries AR. (1988). A versatile video image analysis system for microcirculatory research. Int J Microcirc Clin Exp 7:327-345.
24. Pries AR, Kanzow G, Gaehtgens P. (1983). Microphotometric determination of hematocrit in small vessels. Am J Physiol 245:H167-H177.
25. Secomb T, Hsu R, Pries AR. (2001). Motion of red blood cells in a capillary with an endothelial surface layer: effect of flow velocity. Am J Physiol Heart Circ Physiol 281:H629-H636.
26. Slaaf DW, Arts W, Jeurens JM, Tangelder GJ, Reneman RS. (1984). Electronic measurement of red blood cell velocity and volume flow in microvessels. In Investigative Microtechniques in Medicine and Biology (L Bitensky, Ed.). New York: Dekker, 1.
27. Stein JC, Ellsworth ML. (1992). Microvascular oxygen transport: impact of a left-shifted dissociation curve. Am J Physiol 262:H517-H522.
28. Sullivan SM, Pittman RN. (1982). Hamster retractor muscle: a new preparation for intravital microscopy. Microvasc Res 23:329-335.
29. Tyml K, Budreau CH. (1991). A new preparation of rat extensor digitorum longus muscle for intravital investigation of the microcirculation. Int J Microcirc Clin Exp 10:335-343.
30. Tyml K, Sherebrin MH. (1980). A method for on-line measurements of red cell velocity in microvessels using computerized frame-by-frame analysis of television images. Microvasc Res 20:1-8.