Universal fluorescent labeling of PCR products for DHPLC analysis: Reducing cost and increasing sample throughput

BioTechniques

Volumn 39, Issue 1, 2005, Pages 34-40

  Guipponi, Michel ^a   Herbert, Shane ^a   Toh, Min Yen ^a   Poetter, Karl ^a   Forrest, Susan ^a   Scott, Hamish S ^a  

^a WALTER AND ELIZA HALL INSTITUTE OF MEDICAL RESEARCH   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; GENOMIC DNA;

ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; CHROMATOGRAPHY; COST EFFECTIVENESS ANALYSIS; EXON; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBICITY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; TEMPERATURE; ULTRAVIOLET RADIATION; AUSTRALIA; COMPARATIVE STUDY; COST BENEFIT ANALYSIS; ECONOMICS; EVALUATION; GENETICS; METHODOLOGY; SINGLE NUCLEOTIDE POLYMORPHISM; STAINING;

BACTERIA (MICROORGANISMS);

AUSTRALIA; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COST-BENEFIT ANALYSIS; FLUORESCENT DYES; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; STAINING AND LABELING;

EID: 24944544443     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/05391BM02     Document Type: Article

References (5)

1. Lander, E.S. 1996. The new genomics: global views of biology. Science 274:536-539.
2. Xiao, W. and P.J. Oefner. 2001. Denaturing high-performance liquid chromatography: a review. Hum. Mutat. 17:439-474.
3. Xiao, W., D. Stern, M. Jain, C.G. Huber, and P.J. Oefner. 2001. Multiplex capillary denaturing high-performance liquid chromatography with laser-induced fluorescence detection. BioTechniques 30:1332-1338.
4. Varian, Inc. 2002. Varian 364 Multi-Channel Fluorescence Detector, Product literature. Varian Australia Pty Ltd., Mulgrave, VIC, Australia.
5. Schuelke, M. 2000. An economic method for the fluorescent labeling of PCR fragments. Nat. Biotechnol. 18:233-234.