Extraction of alkaline protease using an aqueous two-phase system from cell free Bacillus subtilis TISTR 25 fermentation broth

Process Biochemistry

Volumn 40, Issue 11, 2005, Pages 3514-3518

  Chouyyok, Wilaiwan ^a,b   Wongmongkol, Nuntiya ^a,c   Siwarungson, Napa ^a   Prichanont, Seeroong ^a  

^a CHULALONGKORN UNIVERSITY   (Thailand)

^b Rajamangala University of Technology Suwanaphumi   (Thailand)

^c THAILAND INSTITUTE OF SCIENTIFIC AND TECHNOLOGICAL RESEARCH   (Thailand)

Author keywords

Alkaline protease; Aqueous two phase system; Cell free fermentation broth; Extraction; PEG1000

Indexed keywords

CELL CULTURE; EXTRACTION; FERMENTATION; PH EFFECTS; POTASSIUM COMPOUNDS; PROTEINS; PURIFICATION;

ALKALINE PROTEASE; AQUEOUS TWO-PHASE SYSTEM; CELL FREE FERMENTATION BROTH; PEG1000;

ENZYMES;

BACILLUS SUBTILIS;

EID: 24944513020     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2005.03.052     Document Type: Article

References (22)

1. T.K. Ng, and W.R. Wenealy Industrial applications of thermostable enzymes T.D. Brock Thermophiles general molecular and applied microbiology 1986 John Wiley New York 197 205
2. R.N. Grebeskova, J.M. Ryshlava, L.G. Fedorova, S.P. Kochetova, O.O. Babloyan, and G.L. Vinogredona Using alkaline protease to intensify the processing of leather raw material Biotechnology 4 6 1988 788 791
3. S.A. Wilson, O.A. Young, T. Coolbear, and R.M. Daniel The use of proteases from extreme thermophiles for meat tenderization Meat Sci 32 1992 93 103
4. S.V. Phadatara, V.V. Deshpande, and M.C. Srinivasan High activity alkaline protease from Conidioboulus coronatus: enzyme production and compatibility with commercial detergents Enzyme Microbiol Technol 15 1 1993 72 75
5. M.T. Cunha, M.R. Aires-Barros, and J.M.S. Cabral Extraction for rapid protein isolation R. Hatti-Kaul B. Mattiasson Isolation and purification of proteins 2003 Marcel Dekker Inc. USA 321 372
6. G.M. Frost Industrial enzyme applications R. Greenshields Resources and applications of biotechnology: a new wave 1989 The Macmillan Press Ltd. 150 184
7. C. Li, J. Bai, W. Li, Z. Cai, and F. Ouyang Optimization of conditions for bacteriocin extraction in PEG/salt aqueous two-phase systems using statistical experimental designs Biotechnol Prog 17 2001 366 368
8. A.S. Limo, R.M. Alegre, and A.J.A. Meirelles Partitioning of pectinolytic enzymes in polyethylene glycol/potassium phosphate aqueous two-phase systems Carbohydr Polym 50 2002 63 68
9. J.C. Marcos, L.P. Fonseca, M.T. Ramalho, and J.M.S. Cabral Application of surface response analysis to the optimization of penicillin acylase purification in aqueous two-phase systems Enzyme Microb Technol 31 2002 1006 1014
10. S. Hotha, and R.M. Banik Production of alkaline protease by Bacillus thuringiensis H14 in aqueous two-phase systems J Chem Technol Biotechnol 69 1997 5 10
11. Y.H. Lee, and H.N. Chang Production of alkaline protease by Bacillus licheniformis in an aqueous two-phase system J Ferment Bioeng 69 2 1990 89 92
12. A. Kaul, and J.A. Asenjo Partition of soluble proteins from E. coli in polyethyleneglycol-salt two-phase systems P.L. Pyle Separations for biotechnology 3rd ed. 1994 Bookcraft London 235 241
13. N.A. Raja, R. Chenyonya, A. Kamaruzaman, B. Mahiran, W.M. Yunus, and A.B. Sallen Purification and characterization of a heat-stable alkaline protease from Bacillus stearothemophilus F1 Appl Microbiol Biotechnol 40 1994 822 827
14. B.Y. Zaslavsky Aqueous two-phase partitioning: physical chemistry and bioanalytical applications 1995 Marcell Dekker Inc. New York
15. M.R. Kula, K.H. Kroner, and H. Hustedt Purification of enzyme by liquid-liquid extraction A. Flechter Advances in biochemical engineering vol. 24 1982 Springer-Verlag Berlin 73 118
16. Wongmongkol, N., An aqueous two-phase system for alkaline protease extraction. M. Eng. thesis, Chulalongkorn University, Bangkok, Thailand; 2001.
17. P.A. Albertsson Partition of cell particles and macromolecules 1986 Wiley New York
18. P.W. Owen W.M. Fogarty Microbial enzymes and biotechnology 1983 Applied Science London 270
19. Y. Xu, M. Vitolo, C.N. Albuquerque, and A. Pessoa Jr. Affinity partitioning of glucose-6-phosphate dehydrogenase and hexokinase in aqueous two-phase systems with free triazine dye ligands J Chromatogr B 780 2002 53 60
20. S.L. Mistry, A. Kaul, J.C. Merchuk, and J.A. Asenjo Mathematical modeling and computer simulation of aqueous two-phase continuous protein extraction J Chromatogr A 741 1996 151 163
21. M.J. Sebastiao, J.M.S. Cabral, and M.R. Aires-Barros Partitioning of recombinant Fusarium solani pisi cutinase in polyethylene glycol-aqueous salt solution two-phase systems J Chromatogr A 668 1994 139 144
22. M.J. Sebastiao, J.M.S. Cabral, and M.R. Aires-Barros Improved purification protocol of a Fusarium solani pisi recombinant cutinase by phase partitioning in aqueous two-phase systems of polyethylene glycol and phosphate Enzyme Microb Technol 18 1996 251 260