An improved method for proteomics studies in C. elegans by fluorogenic derivatization, HPLC isolation, enzymatic digestion and liquid chromatography-tandem mass spectrometric identification

Biomedical Chromatography

Volumn 19, Issue 7, 2005, Pages 556-560

  Masuda, Mayumi ^a   Saimaru, Hiroshi ^b   Takamura, Norio ^b   Imai, Kazuhiro ^b  

^a UNIVERSITY OF TOKYO   (Japan)

^b MUSASHINO UNIVERSITY   (Japan)

Author keywords

7 chloro 4 (dimethylaminoethylaminosulfonyl) 2,1,3 benzoxadiazole; Caenorhabditis elegans; ESI MS MS; Fluorescent derivatization; Proteomics

Indexed keywords

LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR BIOLOGY; PROTEINS;

7-CHLORO-4-(DIMETHYLAMINOETHYLAMINOSULFONYL)-2,1,3-BENZOXADIAZOLE; CAENORHABDITIS ELEGANS; DERIVATIZATIONS; ELECTROSPRAY IONIZATION-MS/MS; ELEGANS; ENZYMATIC DIGESTIONS; FLUORESCENT DERIVATISATION; FLUOROGENICS; PROTEOMIC STUDIES; PROTEOMICS;

ELECTROSPRAY IONIZATION;

7 CHLORO 4 (DIMETHYLAMINOETHYLAMINOSULFONYL) 2,1,3 BENZOXADIAZOLE; ACTIN; ADENINE NUCLEOTIDE TRANSLOCASE; ADENOSINE TRIPHOSPHATASE INHIBITOR; ARGININE KINASE; CAENORHABDITIS ELEGANS PROTEIN; CALRETICULIN; CITRATE SYNTHASE; CYSTATHIONINE GAMMA LYASE; DEOXYRIBONUCLEASE; ELONGATION FACTOR; ELONGATION FACTOR 1; ELONGATION FACTOR 1ALPHA; ENOLASE; FRUCTOSE BISPHOSPHATE ALDOLASE; GLUTAMATE DEHYDROGENASE; GLUTATHIONE TRANSFERASE; HEAT SHOCK PROTEIN 20; HEAT SHOCK PROTEIN 70; HISTONE H2B; IMIDAZOLE DERIVATIVE; MALATE DEHYDROGENASE; MYOSIN HEAVY CHAIN; MYOSIN LIGHT CHAIN; NUCLEOSIDE DIPHOSPHATE KINASE; PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP); RIBONUCLEOPROTEIN; RIBOSOME PROTEIN; TROPOMYOSIN; UNCLASSIFIED DRUG;

ALGORITHM; ANALYTIC METHOD; ARTICLE; CAENORHABDITIS ELEGANS; DERIVATIZATION; ELECTROSPRAY MASS SPECTROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION PROCEDURE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEOMICS; TANDEM MASS SPECTROMETRY;

CAENORHABDITIS ELEGANS;

EID: 24944486228     PISSN: 02693879     EISSN: None     Source Type: Journal    
DOI: 10.1002/bmc.479     Document Type: Article

References (5)

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