Clinical evaluation of an aerolysin-based screening test for paroxysmal nocturnal haemoglobinuria

Cytometry Part B - Clinical Cytometry

Volumn 67, Issue 1, 2005, Pages 13-18

  Peghini, Pietro Edmondo ^a   Fehr, Jörg ^a  

^a UNIVERSITY HOSPITAL ZURICH   (Switzerland)

Author keywords

FLAER; Haemolysis; Immunophenotyping; Paroxysmal nocturnal haemoglobinuria

Indexed keywords

AEROLYSIN; ANTIBODY; CD14 ANTIGEN; CD16 ANTIGEN; CD24 ANTIGEN; CD59 ANTIGEN; CREATINE; DECAY ACCELERATING FACTOR; FLUORESCENT DYE; GLYCOSYLPHOSPHATIDYLINOSITOL; PROTEIN;

AEROMONAS HYDROPHILA; ARTICLE; CONTROLLED STUDY; EVALUATION; FLUORESCENCE ACTIVATED CELL SORTING; HEMOLYSIS; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; PAROXYSMAL NOCTURNAL HEMOGLOBINURIA; PRIORITY JOURNAL; SCREENING TEST;

BACTERIAL TOXINS; CELL SEPARATION; DIAGNOSTIC TECHNIQUES AND PROCEDURES; FLOW CYTOMETRY; FLUORESCENT DYES; GLYCOSYLPHOSPHATIDYLINOSITOLS; HEMOGLOBINURIA, PAROXYSMAL; HUMANS; PORE FORMING CYTOTOXIC PROTEINS; SENSITIVITY AND SPECIFICITY; STATISTICS;

EID: 24744464140     PISSN: 15524949     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.b.20059     Document Type: Article

References (12)

1. Hartmann RC, Jenkins DE. Sugar-water test for paroxysmal nocturnal hemoglobinuria. N Engl J Med 1966;275:155-157.
2. Hartmann RC, Jenkins DE. Arnold AB. Diagnostic specificity of sucrose hemolysis test for paroxysmal nocturnal hemoglobinuria. Blood 1970;35:462-475.
3. van der School CE, Huizinga TW, ET vtV-K, Wijmans R, Pinkster J, dem Borne AE. Deficiency of glycosyl-phosphatidylinositol-linked membrane glycoproteins of leukocytes in paroxysmal nocturnal hemoglobinuria, description of a new diagnostic cytofluorometric assay. Blood 1990;76:1853-1859.
4. Hall SE, Rosse WF. The use of monoclonal antibodies and flow cytometry in the diagnosis of paroxysmal nocturnal hemoglobinuria. Blood 1996;87:5332-5340.
5. Schubert J, Alvarado M, Uciechowski P, et al. Diagnosis of paroxysmal nocturnal haemoglobinuria using immunophenotyping of peripheral blood cells. Br J Haematol 1991;79:487-492.
6. Howard SP, Buckley JT Activation of the hole-forming toxin aerolysin by extracellular processing. J Bacteriol 1985;163:336-340.
7. Garland WJ, Buckley JT The cytolytic toxin aerolysin must aggregate to disrupt erythrocytes, and aggregation is stimulated by human glycophorin. Infect Immun 1988;56:1249-1253.
8. Mukhina G, Buckley T, Brodsky RA. A rapid spectrophotometric screening assay for paroxysmal nocturnal hemoglobinuria. Acta Haematol 2002;107:182-184.
9. Brodsky RA, Mukhina GL, Nelson KL, Lawrence TS, Jones RJ, Buckley JT. Diagnosis of paroxysmal nocturnal hemoglobinuria (PNH) and selection of small PNH populations using a novel GPI-anchor binding toxin, aerolysin. Blood 1998;92:472A.
10. Brodsky RA, Mukhina GL, Li S, et al. Improved detection and characterization of paroxysmal nocturnal hemoglobinuria using fluorescent aerolysin. Am J Clin Pathol 2000;114:459-466.
11. Fehr J, Knob M. Comparison of red-cell creatine level and reticulocyte count in appraising the severity of hemolytic processes. Blood 1979;53:966-976.
12. Dunn DE, Tanawattanacharoen P, Boccuni P, et al. Paroxysmal noctumal hemoglobinuria cells in patients with bone marrow failure syndromes. Ann Intern Med 1999;131:401-408.