Physical exercise induces oxidation of plasma protein thiols to cysteine mixed disulfides in humans

Journal of Health Science

Volumn 48, Issue 5, 2002, Pages 399-403

  Inayama, Takayo ^a,b   Kashiba, Misato ^a   Oka, Jun ^a   Higuchi, Mitsuru ^a   Umegaki, Keizo ^a   Saito, Makoto ^c   Yamamoto, Yorihiro ^d   Matsuda, Mitsuo ^e  

^a NATIONAL INSTITUTE OF HEALTH AND NUTRITION   (Japan)

^b Sendai Shirayuri Women's College   (Japan)

^c OTSUMA WOMEN'S UNIVERSITY   (Japan)

^d UNIVERSITY OF TOKYO   (Japan)

^e UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Cysteine mixed disulfide; Human; Physical exercise; Plasma protein thiol

Indexed keywords

DISULFIDE; DITHIOTHREITOL; GAMMA GLUTAMYLTRANSFERASE; GLUTATHIONE; PEPTIDASE; PLASMA PROTEIN; THIOL DERIVATIVE;

ARTICLE; CONTROLLED STUDY; EXERCISE; FEMALE; HUMAN; HYDROLYSIS; OXIDATION; OXIDATIVE STRESS; PLASMA; PROBABILITY; REDUCTION;

EID: 24744447417     PISSN: 13449702     EISSN: None     Source Type: Journal    
DOI: 10.1248/jhs.48.399     Document Type: Article

References (20)

1. Packer, L. (1997) Oxidants, antioxidant nutrients and the athlete. J. Sports Sci., 15, 353-363.
2. Ji, L. L. (1999) Antioxidants and oxidative stress in exercise. Proc. Soc. Exp. Biol. Med., 222, 283-292.
3. Sen, C. K. and Packer, L. (2000) Thiol homeostasis and supplements in physical exercise. Am. J. Clin. Nutr., 72, 653S-669S.
4. Jones, D. P., Carlson, J. L., Mody, V. C., Cai, J., Lynn, M. J. and Sternberg, P. (2000) Redox state of glutathione in human plasma. Free. Radic. Biol. Med., 28, 625-635.
5. Inoue, M., Minamiyama, Y., Yu, H., Yamamasu, S., Inoue, K. and Sato, E. (1995) Biochemical and clinical aspects of extracellular glutathione and related thiols. In Biothiols in health and disease (Packer, L. and Cadenas, E., eds.), Marcel Dekker, New York, pp. 287-304.
6. Inayama, T., Kumagai, Y., Sakane, M., Saito, M. and Matsuda, M. (1996) Plasma protein-bound sulfhydryl group oxidation in humans following a full marathon race. Life Sci., 59, 573-578.
7. Pyke, S., Lew, H. and Quintanilha, A. (1986) Severe depletion in liver glutathione during physical exercise. Biochem. Biophys. Res. Commun., 139, 926-931.
8. Inayama, T., Oka, J., Kahiba, M., Saito, M., Higuchi, M., Umegaki, K., Yamamoto, Y. and Matsuda, M. (2002) Moderate physical exercise induces the oxidation of human blood protein thiols. Life Sci., 70, 2039-2046.
9. Sedlak, J. and Lindsay, R. H. (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal. Biochem., 25, 192-205.
10. Yasuhara, T. and Nokihara, K. (1998) Quantitative determination of biological sulfhydryl groups by postcolumn derivatization and elucidation of microheterogeneity of serum albumins. Anal. Chem., 70, 3505-3509.
11. Meister, A. (1983) Selective modification of glutathione metabolism. Science, 220, 472-477.
12. Lash, L. H. and Jones, D. P. (1985) Distribution of oxidized and reduced forms of glutathione and cysteine in rat plasma. Arch. Biochem. Biophys., 240, 583-592.
13. Libenson, L. and Jena, M. (1967) Uptake of glutathione by serum albumin of normal and tumor-bearing mice. Cancer Res, 27, 1196-1201.
14. Meister, A., Tate, S. S. and Griffith, O. W. (1981) Gamma-glutamyl transpeptidase. Methods Enzymol., 77, 237-253.
15. Inoue, M., Saito, Y., Hirata, E., Morino, Y. and Nagase, S. (1987) Regulation of redox states of plasma proteins by metabolism and transport of glutathione and related compounds. J. Protein Chem., 6, 207-225.
16. Brown, M. D., Srinivasan, M., Hogikyan, R. V., Dengel, D. R., Glickman, S. G., Galecki, A. and Supiano, M. A. (2000) Nitric oxide biomarkers increase during exercise-induced vasodilation in the forearm. Int. J. Sports Med., 21, 83-89.
17. Stamler, J. S., Jaraki, O., Osborne, J., Simon, D. I., Keaney, J., Vita, J., Singel, D., Valeri, C. R. and Loscalzo, J. (1992) Nitric oxide circulates in mammalian plasma primarily as an S-nitroso adduct of serum albumin. Proc. Natl. Acad. Sci. U.S.A., 89, 7674-7677.
18. Klatt, P. and Lamas, S. (2000) Regulation of protein function by S-glutathiolation in response to oxidative and nitrosative stress. Eur. J. Biochem., 267, 4928-4944.
19. Saville, B. (1958) A scheme for the colorimetric determination of microgram amounts of thiols. Analyst (London), 83, 670-672.
20. King, T. P. (1961) On the sulfhydryl group of human plasma albumin. J. Biol. Chem., 236, PC5.