Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies: Alteration of substrate specificity by replacement of Asn149 with Asp

FEBS Journal

Volumn 272, Issue 17, 2005, Pages 4540-4548

  López, Vasthi ^a   Alarcón, Ricardo ^a   Orellana, María S ^a   Enríquez, Paula ^a   Uribe, Elena ^a   Martínez, José ^a   Carvajal, Nelson ^a  

^a UNIVERSITY OF CONCEPCIÓN   (Chile)

Author keywords

Agmatinase activity; Arginase II; Histidine; Human; Manganese ions

Indexed keywords

AGMATINE; ARGINASE; ARGINASE II; ARGININE; ASPARAGINE; ASPARTIC ACID; EDETIC ACID; HISTIDINE; MANGANESE; METAL ION; TRYPTOPHAN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CHROMATOGRAPHY; CONFORMATIONAL TRANSITION; DIALYSIS; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE; GENE MUTATION; MUTANT; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; SPECTRAL SENSITIVITY; WILD TYPE;

AMINO ACID SUBSTITUTION; ARGINASE; BASE SEQUENCE; DNA; HUMANS; KINETICS; MANGANESE; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 24644487030     PISSN: 1742464X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2005.04874.x     Document Type: Article

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