Establishment of a quantitative real-time PCR assay for the quantification of apple proliferation phytoplasmas in plants and insects

Acta Horticulturae

Volumn 657, Issue , 2004, Pages 415-420

  Jarausch, W ^a   Peccerella, T ^a   Schwind, N ^a   Jarausch, B ^a   Krczal, G ^a  

^a Centrum Grüne Gentechnik   (Germany)

Author keywords

Apple; Apple proliferation phytoplasma; Psyllid; Quantitative PCR

Indexed keywords

EID: 24644448645     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/ActaHortic.2004.657.66     Document Type: Conference Paper

References (9)

1. Berges, R., Rott, M. and Seemüller, E. 2000. Range of phytoplasma concentrations in various plant hosts as determined by competitive polymerase chain reaction. Phytopathology 90: 1145-1152.
2. Frisinghelli, C., Delatti, L., Grando, M.S., Forti, D. and Vindimian, M.E. 2000. Cacopsylla costalis (Flor 1861), as a vector of apple proliferation in Trentino. Journal of Phytopathology 148: 425-431.
3. Jarausch, B., Schwind, N., Jarausch, W., Krczal, G., Seemüller, E. and Dickler E. 2003a. First report of Cacopsylla picta as a vector for apple proliferation phytoplasma in Germany. Plant Disease 87: 101.
4. Jarausch, B. 2003b. Welche Rollen spielen Blattsaugerarten bei der Übertragung von Apfeltriebsucht-Phytoplasmen in deutschen Apfelanlagen? Obstbau 4: 205-206.
5. Jarausch, W., Lansac, M. and Dosba, F. 1996. Long-term maintenance of non-culturable apple proliferation phytoplasmas in their micropropagated natural host plant. Plant Pathol. 45, 778-786.
6. Jarausch, W., Saillard, C., Dosba, F. and Bové, J.M. 1994. Differentiation of mycoplasmalike organisms (MLOs) in European fruit trees by PCR using specific primers derived from the sequence of a chromosomal fragment of the apple proliferation MLO. Applied and Environmental Microbiology 60: 2916-2923.
7. Kartte, S. and Seemüller, E. 1991. Susceptibility of grafted Malus taxa and hybrids to apple proliferation disease. J. Phytopath. 131:137-148.
8. Maixner, M., U. Ahrens and Seemüller, E. 1995. Detection of the German grapevine yellows (Vergilbungskrankheit) MLO in grapevine, alternative hosts and a vector by a specific PCR procedure. European Journal of Plant Pathology 101: 241-250.
9. Tedeschi, R., Bosco, D. and Alma, A. 2002. Population dynamics of Cacopsylla melanoneura (Homoptera: Psyllidae), a vector of apple proliferation phytoplasma in Northwestern Italy. J. Econ. Entomol. 95: 544-551.