Continuous assays of glutaminyl cyclase: From development to application

Spectroscopy

Volumn 18, Issue 2, 2004, Pages 363-373

  Schilling, Stephan ^a   Demuth, Hans Ulrich ^a  

^a PROBIODRUG AG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMINES; AMMONIA; ASSAYS; CATALYSIS; CHROMOPHORES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PH EFFECTS;

ENZYMATIC PROPERTIES; GLUTAMINYL CYCLASE; PEPTIDES;

ENZYMES;

GLUTAMATE DEHYDROGENASE; GLUTAMYL AMINOPEPTIDASE;

CHROMATOPHORE; CONFERENCE PAPER; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME DEGRADATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PHOTOMETRY; RADIOIMMUNOASSAY;

EID: 2442693954     PISSN: 07124813     EISSN: None     Source Type: Journal    
DOI: 10.1155/2004/413050     Document Type: Conference Paper

References (27)

1. M. Messer, Enzymatic cyclization of L-glutamine and L-glutaminyl peptides, Nature 4874 (1963), 1299.
2. M. Messer and M. Ottesen, Isolation and properties of glutamine cyclotransferase of dried papaya latex, Biochim. Biophys. Acta 92 (1964), 409-411.
3. W.H.J. Busby, G.E. Quackenbush, J. Humm, W.W. Youngblood and J.S. Kizer, An enzyme(s) that converts glutaminyl-peptides into pyroglutamyl-peptides. Presence in pituitary, brain, adrenal medulla, and lymphocytes, J. Biol. Chem. 262 (1987), 8532-8536.
4. W.H. Fischer and J. Spiess, Identification of a mammalian glutaminyl cyclase converting glutaminyl into pyroglutamyl peptides, Proc. Natl. Acad. Sci. USA 84 (1987), 3628-3632.
5. S.W. Dahl, C. Slaughter, C. Lauritzen, R.C. Bateman, Jr., I. Connerton and J. Pedersen, Carica papaya glutamine cyclotransferase belongs to a novel plant enzyme subfamily: cloning and characterization of the recombinant enzyme, Protein Expr. Purif. 20 (2000), 27-36.
6. E. Van Coillie, P. Proost, I. Van Aelst, S. Struyf, M. Polfliet, I. De Meester, D.J. Harvey, J. Van Damme and G. Opdenakker, Functional comparison of two human monocyte chemotactic protein-2 isoforms, role of the amino-terminal pyroglutamic acid and processing by CD26/dipeptidyl peptidase IV, Biochemistry 37 (1998), 12672-12680.
7. G.N. Abraham and D.N. Podell, Pyroglutamic acid. Non-metabolic formation, function in proteins and peptides, and characteristics of the enzymes effecting its removal, Mol. Cell. Biochem. 38 (Spec. No) (1981), 181-190.
8. S.A. Hinke, J.A. Pospisilik, H.-U. Demuth, S. Manhart, K. Kühn-Wache, T. Hoffmann, E. Nishimura, R.A. Pedersen and C.H.S. McIntosh, Dipeptidyl peptidase IV (DPIV/CD26) degradation of Glucagon, J. Biol. Chem. 275 (2000), 3827-3834.
9. S. Zerhouni, A. Amrani, M. Nijs, N. Smolders, M. Azarkan, J. Vincentelli and Y. Looze, Purification and characterization of papaya glutamine cyclotransferase, a plant enzyme highly resistant to chemical, acid and thermal denaturation, Biochim. Biophys. Acta 1387 (1998), 275-290.
10. T. Pohl, M. Zimmer, K. Mugele and J. Spiess, Primary structure and functional expression of a glutaminyl cyclase, Proc. Natl. Acad. Sci. USA 88 (1991), 10059-10063.
11. A.P. Consalvo, S.D. Young, B.N. Jones and P.P. Tamburini, A rapid fluorometric assay for N-terminal glutaminyl cyclase activity using high-performance liquid chromatography, Anal. Biochem. 175 (1988), 131-138.
12. M.Y. Gololobov, W. Wang and R.C.J. Bateman, Substrate and inhibitor specificity of glutamine cyclotransferase (QC), Biol. Chem. Hoppe Seyler 377 (1996), 395-398.
13. K.A. Oberg, J.M. Ruysschaert, M. Azarkan, N. Smolders, S. Zerhouni, R. Wintjens, A. Amrani and Y. Looze, Papaya glutamine cyclase, a plant enzyme highly resistant to proteolysis, adopts an all-beta conformation, Eur. J. Biochem. 258 (1998), 214-222.
14. S. Schilling, T. Hoffmann, F. Rosche, S. Manhart, C. Wasternack and H.U. Demuth, Heterologous expression and characterization of human glutaminyl cyclase: evidence for a disulfide bond with importance for catalytic activity, Biochemistry 41 (2002), 10849-10857.
15. J.B. Koger, J. Humm and J.S. Kizer, Assay of glutaminylpeptide cyclase, Methods Enzymol. 168 (1989), 358-365.
16. R.C.J. Bateman, A spectrophotometric assay for glutaminyl-peptide cyclizing enzymes, J. Neurosci. Methods 30 (1989), 23-28.
17. M.Y. Gololobov, I. Song, W. Wang and R.C.J. Bateman, Steady-state kinetics of glutamine cyclotransferase, Arch. Biochem. Biophys. 309 (1994), 300-307.
18. W.R. McClure, A kinetic analysis of coupled enzyme assays, Biochemistry 8 (1969), 2782-2786.
19. K. Fujiwara, R. Kobayashi and D. Tsuru, The substrate specificity of pyrrolidone carboxylyl peptidase from Bacillus amyloliquefaciens, Biochim. Biophys. Acta 570 (1979), 140-148.
20. K. Fujiwara and D. Tsuru, New chromogenic and fluorogenic substrates for pyrrolidonyl peptidase, J. Biochem. (Tokyo) 83 (1978), 1145-1149.
21. D. Tsuru, K. Fujiwara and K. Kado, Purification and characterization of L-pyrrolidonecarboxylate peptidase from Bacillus amyloiliquefaciens, J. Biochem. (Tokyo) 84 (1978), 467-476.
22. P.M. Cummins and B. O'Connor, Pyroglutamyl peptidase: an overview of the three known enzymatic forms, Biochim. Biophys. Acta 1429 (1998), 1-17.
23. S. Schilling, T. Hoffmann, M. Wermann, U. Heiser, C. Wasternack and H.-U. Demuth, Continuous spectrometric assays for glutaminyl cyclase activity, Anal. Biochem. 303 (2002), 49-56.
24. C. Frieden, Glutamic dehydrogenase. III. The order of substrate addition in the enzymatic reaction, J. Biol. Chem. 234 (1959), 2891-2896.
25. K.J. Ellis and J.F. Morrison, Buffers of constant ionic strength for studying pH-dependent processes, Methods Enzymol. 87 (1982), 405-426.
26. S. Schilling, S. Manhart, T. Hoffmann, H.-H. Ludwig, C. Wasternack and H.-U. Demuth, Substrate specificity of glutaminyl cyclases from plants and animals, Biol. Chem. 384 (2003), 1583-1592.
27. S. Schilling, A.J. Niestroj, J.-U. Rahfeld T. Hoffmann, M. Wermann, K. Zunkel, C. Wasternack and H.-U. Demuth, Potent and specific inhibition of human glutaminyl cyclase by imidazole derivatives reveals it as metalloenzyme, J. Biol. Chem. 278 (2003), 49773-49779.