Calmodulin antagonist W-7 inhibits de novo synthesis of cholesterol and suppresses secretion of de novo synthesized and preformed lipids from cultured hepatocytes

Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

Volumn 1682, Issue 1-3, 2004, Pages 92-101

  Rasouli, Mehdi ^a,b   Trischuk, Timothy C ^a   Lehner, Richard ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

ACAT; acyl CoA:cholesterol acyltransferase; Apo; apoB; apolipoprotein; bovine serum albumin; BSA; CE; Ch; Cholesterol; cholesterol; Cholesteryl ester; cholesteryl ester; DMEM; Dulbecco's modified Eagle medium; Hepatocyte; McArdle RH7777; PC; Triacylglycerol; VLDL; W 7

Indexed keywords

ACETIC ACID; CALMODULIN INHIBITOR; CARBON 14; CHOLESTEROL; CHOLESTEROL ESTER; GLYCEROL; GLYCEROLIPID; MEVALONOLACTONE; N (6 AMINOHEXYL) 5 CHLORO 1 NAPHTHALENESULFONAMIDE; OLEIC ACID; PHOSPHATIDYLCHOLINE; TRIACYLGLYCEROL; TRITIUM;

ANIMAL CELL; ARTICLE; CELL STRAIN; CONTROLLED STUDY; DOSE RESPONSE; DRUG EFFECT; ESTERIFICATION; LIPOGENESIS; LIVER CELL; NONHUMAN; PRIORITY JOURNAL; RAT; STATISTICAL SIGNIFICANCE;

ANIMALS; APOLIPOPROTEINS B; CALMODULIN; CARBON RADIOISOTOPES; CHOLESTEROL; GLYCERIDES; HEPATOCYTES; LIPOPROTEINS, VLDL; MEVALONIC ACID; OLEIC ACID; RATS; SULFONAMIDES; TRITIUM;

BOVINAE; CICONIIFORMES;

EID: 2442691300     PISSN: 13881981     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbalip.2004.02.002     Document Type: Article

References (47)

1. Roufogalis B.D., Minocherhomjee A.M., AI-Jobove A. Pharmacological antagonism of calmodulin. Can. J. Biochem. Cell Biol. 6:1983;935-987
2. Kosugi K., Harano Y., Nakano T., Suzuki M., Kashiwagi A., Shigeta Y. Mechanism of adrenergic stimulation of hepatic ketogenesis. Metabolism. 32:1983;1081-1087
3. Nishizawa Y., Okui Y., Inaba M., Okuno S., Yukioka K., Miki T., Watanabe Y., Morrii H. Calcium/calmodulin-mediated action of calcitonin on lipid metabolism in rats. J. Clin. Invest. 82:1988;1165-1172
4. Pelech S.L., Vance D.E. Trifluoperazine and chlorpromazine inhibit phosphatidylcholine biosynthesis and CTP: phosphocholine cytidylyltransferase in HeLa cells. Biochim. Biophys. Acta. 795:1984;441-446
5. Hopewell R., Martin Sanz P., Martin A., Saxton J., Brindley D.N. Regulation of the translocation of phosphatidate phosphohydrolase between cytosol and the endoplasmic reticulum of rat liver. Effects of unsaturated fatty acids, spermine, nucleotides, albumin and chlorpromazine. Biochem. J. 232:1985;482-491
6. 3H]glycerol in the primordial human placenta. Placenta. 16:1995;347-358
7. Field F.J., Born E., Murthy S., Mathur S.N. Transport of cholesterol from the endoplasmic reticulum to plasma membrane is constitutive in CaCo-2 cells and differs from the transport of plasma membrane cholesterol to the endoplasmic reticulum. J. Lipid Res. 30:1998;333-343
8. Field F.J., Born E., Chen E., Murthy S., Mathur S.N. Esterification of plasma membrane cholesterol and triacylglycerol rich lipoprotein. Secretion in CaCo-2 cells: possible role of P-glycoprotein. J. Lipid Res. 36:1995;1533-1543
9. 24-reductase. Lipids. 22:1987;261-265
10. Mehta U., Kaul D. Effects of trifluoperazine on cholesterol metabolism of smooth muscle cells exposed to hypercholesterolemic medium in vitro. Biochem. Med. Metabol. Biol. 44:1990;151-158
11. Lehner R., Cui Z., Vance D.E. Subcellular, developmental expression and characterization of a liver triacylglycerol hydrolase. Biochem. J. 338:1999;161-168
12. Lehner R., Vance D.E. Cloning and expression of a cDNA encoding a hepatic microsomal lipase that mobilized stored triacylglycerol. Biochem. J. 343:1999;1-10
13. Gilham D., Ho S., Rasouli M., Martres P., Vance D.E., Lehner R. Inhibitors of hepatic microsomal triacylglycerol hydrolase decrease VLDL secretion. FASEB J. 17:2003;1685-1687
14. Folch J., Lees M., Sloane-Stanley G.H. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:1957;497-509
15. Klausner H., Soler-Argilaga C., Heimberg M. Effects of dibutyryl-cAMP on hepatic metabolism of free fatty acids. Metabolism. 27:1978;13-25
16. Bronstad G.O., Christofferson T., Johansen E.J., Oye I. Effect of prostaglandins and hormones on cyclic AMP formation in rat hepatomas and liver tissue. Br. J. Cancer. 38:1978;737-744
17. Leichtling B.H., Su Y.F., Wimalasena J., Harden T.K., Wolfe B.B., Wicks W.D. Studies of cAMP metabolism in cultured hepatoma cells: presence of functional adenylate cyclase despite low cAMP content and lack of hormonal responsiveness. J. Cell. Physiol. 96:1978;215-223
18. Yao Z., McLeod R.S. Synthesis and secretion of hepatic apolipoprotein B containing lipoprotein. Biochim. Biophys. Acta. 1212:1994;152-166
19. Fisher E.A., Ginsberg H.N. Complexity in the secretory pathway: the assembly and secretion of apolipoprotein B-containing lipoproteins. J. Biol. Chem. 277:2002;17377-17380
20. Davis R.A., Thrift R.N., Wu C.C., Howell K.E. Apolipoprotein B is both integrated into and translocated across the endoplasmic reticulum membrane. J. Biol. Chem. 265:1990;10005-10011
21. Yao Z., Khai T., McLeod R.S. Intracellular degradation of newly synthesized apolipoprotein B. J. Lipid Res. 38:1997;1937-1953
22. Wu X., Sakata N., Lele K.M., Zhou M., Jiang H., Ginsberg H.N. A two-site model for apoB degradation in HepG2 cells. J. Biol. Chem. 272:1997;11575-11580
23. Boren J., Rustaeus S., Wettesten M., Anderson A., Wiklund A., Olofsson S.O. Influence of triacylglycerol biosynthesis rate on the assembly of apoB-100 containing lipoproteins in HepG2 cells. Arterioscler. Thromb. 13:1993;1743-1754
24. Francone O.L., Kalopissis A.-D., Griffaton G. Contribution of cytoplasmic storage triacylglycerol to VLDL in isolated rat hepatocytes. Biochim. Biophys. Acta. 1002:1989;28-36
25. Cianflone K.M., Tasruel Z., Rodriguez M.A., Vas D., Sniderman A.D. Regulation of apoB secretion from HepG2 cells, evidence for a critical role for cholesteryl ester synthesis in the response to a fatty acid challenge. J. Lipid Res. 31:1990;2045-2055
26. Musanti R., Giorgini L., Lovisolo P., Pirillo A., Chiari A., Ghiselli G. Inhibition of acyl CoA:cholesterol acyltransferase decreases apolipoprotein B-100 containing lipoprotein secretion from HepG2 cells. J. Lipid Res. 37:1996;1-14
27. Yao Z., Vance D.E. The active synthesis of phosphatidylcholine is required for very low density lipoprotein secretion from rat hepatocytes. J. Biol. Chem. 263:1988;2998-3004
28. Vermeulen P.S., Lingrell S., Yao Z., Vance D.E. Phosphatidylcholine biosynthesis is required for secretion of truncated apolipoprotein Bs from McArdle RH7777 cells only when a neutral lipid core is formed. J. Lipid Res. 38:1997;447-458
29. Verkade H.J., Fast D.G., Rusinol A.E., Scraba D.G., Vance D.E. Impaired biosynthesis of phosphatidylcholine causes a decrease in the number of VLDL particles in the Golgi but not the endoplasmic reticulum of rat liver. J. Biol. Chem. 268:1993;24990-24996
30. Thompson G.R., Naoumova R.P., Watts G.F. Role of cholesterol in regulating apolipoprotein B secretion by the liver. J. Lipid Res. 37:1996;439-447
31. Khan B., Wilcox H.G., Heimberg M. Cholesterol is required for secretion of VLDL by rat liver. Biochem. J. 259:1989;807-816
32. Fungwe T.V., Cagen L., Wilcox H.G., Heimberg M. Regulation of hepatic secretion of VLDL by dietary cholesterol. J. Lipid Res. 33:1992;179-191
33. Wiggins D., Gibbons G.F. The lipolysis/esterification cycle of hepatic triacylglycerol. Its role in the secretion of very-low-density lipoprotein and its response to hormones and sulphonylureas. Biochem. J. 284:1992;457-462
34. 6]ethanol. J. Lipid Res. 37:1996;262-274
35. Lankester D.L., Brown A.M., Zammit V.A. Use of cytosolic triacylglycerol hydrolysis products and of exogenous fatty acid for the synthesis of triacylglycerol secreted by cultured rat hepatocytes. J. Lipid Res. 39:1998;1889-1895
36. Gibbons G.F., Islam K., Pease R.J. Mobilization of triacylglycerol stores. Biochim. Biophys. Acta. 1483:2000;37-57
37. Brown A., Wiggins D., Gibbons G.F. Manipulation of cholesterol and cholesteryl ester synthesis has multiple effects on the metabolism of apolipoprotein B and the secretion of VLDL by primary hepatocyte cultures. Biochim. Biophys. Acta. 1440:1999;253-265
38. Wu X., Sakata N., Liu E., Ginsberg H.N. Evidence for a lack of regulation of the assembly and secretion of apolipoprotein B-containing lipoprotein from HepG2 cells by cholesteryl ester. J. Biol. Chem. 269:1994;12375-12382
39. Field F.J., Born E., Mathur S.N. Triacylglycerol-rich lipoprotein cholesterol is derived from the plasma membrane in CaCo-2 cells. J. Lipid Res. 36:1995;2651-2660
40. Masson M., Spezzatti B., Chapman J., Battisti C., Baumann N. Calmodulin antagonists chlorpromazine and W-7 inhibit exogenous cholesterol esterification and sphingomyelinase activity in human skin fibroblast cultures. J. Neurosci. Res. 31:1992;84-88
41. Maziere J.C., Maziere C., Gardette J., Routier J.D., Wolf C., Rainteau D., Polonovski J. Effects of phenothiazines on low density lipoprotein metabolism in cultured human fibroblasts. FEBS Lett. 162:1983;396-399
42. Maziere C., Maziere J.C., Mora L., Auclair M., Polonovski J. Trifluoperazine increase fatty acid turnover in phospholipids in cultured human fibroblasts. Lipids. 23:1988;419-423
43. Noga A.A., Zhao Y., Vance D.E. An unexpected requirement for phosphatidylethanolamine N-methyltransferase in the secretion of very low density lipoproteins. J. Biol. Chem. 277:2002;42358-42365
44. Bjornsson O.G., Sparks J.D., Sparks C.E., Gibbons G.F. Prostaglandins suppress VLDL secretion in primary rat hepatocyte cultures: relationships to hepatic calcium metabolism. J. Lipid Res. 33:1992;1017-1027
45. Lodish H.F., Kong N. Perturbation of cellular calcium blocks exit of secretory proteins from the rough endoplasmic reticulum. J. Biol. Chem. 265:1990;10893-10899
46. Stein O., Stein Y. Colchicine-induced inhibition of VLDL release by rat liver in vivo. Biochim. Biophys. Acta. 306:1973;142-147
47. Brindle N.J., Ontko J.A. Suppression of triglyceride secretion by epinephrine in isolated rat hepatocytes. Biochem. Biophys. Res. Commun. 141:1986;191-197