Application of a multiplex pbp2b and pbp2x PCR for prediction of penicillin resistance in Streptococcus pneumoniae [4]

Journal of Antimicrobial Chemotherapy

Volumn 53, Issue 5, 2004, Pages 890-891

  Ho, P L ^a   Wong, River C W ^b   Chow, Frankie K H ^a   Cheung, May Y M ^a   Wong, Samson S Y ^a   Yam, W C ^a   Que, T L ^b  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

^b TUEN MUN HOSPITAL   (Hong Kong)

Author keywords

Molecular diagnostic techniques; Polymerase chain reaction; Streptococcus pneumoniae

Indexed keywords

AMINO ACID; MEMBRANE ANTIGEN; NUCLEOTIDE; PENICILLIN BINDING PROTEIN; PENICILLIN BINDING PROTEIN 2B; PENICILLIN BINDING PROTEIN 2X; PENICILLIN G; PRIMER DNA; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANALYTICAL EQUIPMENT; ANTIBIOTIC SENSITIVITY; BACTERIAL STRAIN; BACTERIUM ISOLATE; BIOASSAY; CHILD; CONTROLLED STUDY; DNA TEMPLATE; GENE MUTATION; HUMAN; LETTER; MINIMUM INHIBITORY CONCENTRATION; NASOPHARYNX; NONHUMAN; NUCLEOTIDE SEQUENCE; PENICILLIN RESISTANCE; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; SEQUENCE ANALYSIS; STREPTOCOCCUS PNEUMONIAE;

AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; CARRIER PROTEINS; HEXOSYLTRANSFERASES; MURAMOYLPENTAPEPTIDE CARBOXYPEPTIDASE; MUTATION; PENICILLIN RESISTANCE; PENICILLIN-BINDING PROTEINS; PEPTIDYL TRANSFERASES; PREDICTIVE VALUE OF TESTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STREPTOCOCCUS PNEUMONIAE;

EID: 2442690421     PISSN: 03057453     EISSN: None     Source Type: Journal    
DOI: 10.1093/jac/dkh216     Document Type: Letter

References (8)

1. du-Plessis, M., Smith, A. M. & Klugman, K. P. (1998). Rapid detection of penicillin-resistant Streptococcus pneumoniae in cerebrospinal fluid by a seminested-PCR strategy. Journal of Clinical Microbiology 36, 453-7.
2. Nagai, K., Matsuo, Y., Tsumura, N. et al. (2000). Antimicrobial susceptibilities and serotypes of Streptococcus pneumoniae in southwestern Japan and correlation of penicillin-binding protein 2b and 2x mutations in susceptibilities of penicillin G and cefotaxime. Diagnostic Microbiology and Infectious Disease 37, 107-13.
3. Tang, Y. W., Li, H. J., Griffin, J. P. et al. (2002). Rapidly increasing prevalence of penicillin-resistant Streptococcus pneumoniae in middle Tennessee: a 10-year clinical and molecular analysis. Journal of Clinical Microbiology 40, 395-9.
4. Chiu, S. S., Ho, P. L., Chow, F. K. et al. (2001). Nasopharyngeal carriage of antimicrobial-resistant Streptococcus pneumoniae among young children attending 79 kindergartens and day care centers in Hong Kong. Antimicrobial Agents and Chemotherapy 45, 2765-70.
5. Verhelst, R., Kaijalainen, T., De Baere, T. et al. (2003). Comparison of five genotypic techniques for identification of optochin-resistant pneumococcus-like isolates. Journal of Clinical Microbiology 41, 3521-5.
6. Dowson, C. G., Hutchison, A. & Spratt, B. G. (1989). Extensive re-modelling of the transpeptidase domain of penicillin- binding protein 2B of a penicillin-resistant South African isolate of Streptococcus pneumoniae. Molecular Microbiology 3, 95-102.
7. Krauss, J., van der Linden, M., Grebe, T. et al. (1996). Penicillin-binding proteins 2x and 2b as primary PBP targets in Streptococcus pneumoniae. Microbial Drug Resistance 2, 183-6.
8. Hakenbeck, R., Kaminski, K., Konig, A. et al. (2000). Penicillin-binding proteins in beta-lactam-resistant Streptococcus pneumoniae. In Streptococcus pneumoniae: Molecular Biology and Mechanisms of Disease (Tomasz, A., Ed.), pp. 433-42. Mary Ann Liebert, Inc., New York, NY, USA.