Immunoelectron microscopy study of polyamines using a newly prepared monoclonal antibody against spermidine: Use of a mixture of glutaraldehyde and paraformaldehyde as a cross-linking agent in the preparation of the antigen

Journal of Biochemistry

Volumn 135, Issue 4, 2004, Pages 501-507

  Tanabe, Toshio ^a   Shin, Masashi ^b   Fujiwara, Kunio ^b  

^a NIPPON KAYAKU CO LTD   (Japan)

^b SOJO UNIVERSITY   (Japan)

Author keywords

Glutaraldehyde; Immunoelectron microscopy; Karnovsky; Monoclonal antibody; Paraformaldehyde; Spermidine

Indexed keywords

ACETYLSPERMINE; AMINO ACID DERIVATIVE; ANTIGEN; BOVINE SERUM ALBUMIN; GLUTARALDEHYDE; IMMUNOGLOBULIN G3; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY ASPD 19; N1 ACETYLSPERMIDINE; N8 ACETYLSPERMIDINE; PARAFORMALDEHYDE; PEROXIDASE; POLYAMINE DERIVATIVE; SODIUM BOROHYDRIDE; SPERMIDINE; UNCLASSIFIED DRUG;

ADSORPTION; ANIMAL CELL; ANTIBODY SPECIFICITY; ARTICLE; CHEMICAL STRUCTURE; CONJUGATION; CROSS LINKING; CYTOPLASM; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; IMMUNOCYTOCHEMISTRY; IMMUNOELECTRON MICROSCOPY; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; MOTONEURON; MOUSE; NONHUMAN; ROUGH ENDOPLASMIC RETICULUM; SPINAL CORD;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; BOROHYDRIDES; CROSS-LINKING REAGENTS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; FORMALDEHYDE; GLUTARAL; HAPTENS; HYBRIDOMAS; IMMUNOGLOBULIN G; IMMUNOHISTOCHEMISTRY; INTESTINAL MUCOSA; MALE; MICE; MICE, INBRED BALB C; MICROSCOPY, IMMUNOELECTRON; MOTOR NEURONS; PANCREAS; POLYAMINES; POLYMERS; RATS; RATS, WISTAR; SERUM ALBUMIN, BOVINE; SPERMIDINE; SPERMINE; SPINAL CORD; TISSUE FIXATION; VACCINATION;

ANIMALIA; BOVINAE;

EID: 2442687679     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvh066     Document Type: Article

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