Purification and characterization of chymotrypsin inhibitor CI-3 from hemolymph of silkworm, Bombyx mori

Journal of the Faculty of Agriculture, Kyushu University

Volumn 49, Issue 1, 2004, Pages 93-99

  Zhao, Qiaoling ^a,b   He, Ningjia ^a   Shirai, Koji ^a   Fujii, Hiroshi ^a   Banno, Yutaka ^a   Yamamoto, Kohji ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Bombyx mori; Chymotrypsin inhibitor; Hemolymph; Protein purification

Indexed keywords

BOMBYX; BOMBYX MORI;

EID: 2442540423     PISSN: 00236152     EISSN: None     Source Type: Journal    
DOI: 10.5109/4569     Document Type: Article

References (18)

1. Brizin, J., T. Popuic and V. Tunk 1984 Human cystatin, a new protein inhibitor and cysteine proteinase. Biochem. Biophys. Res. Commun., 118: 103-109
2. Deng, L. R., H. Fujii, H. Aratake, Y. Kawaguchi, and K. Koga 1990 Isolation and properties of two allelic chymotrypsin inhibitors from the hemolymph of the silkworm, Bombyx mori. Insect Biochem., 20: 531-536
3. Edman, P., and G. Begg, 1967 Automated equipment for sequence determinations. Eur. J. Biochem., 1: 80-91
4. Fujii, H., H. Aratake, L. R. Deng, M. Nakamura, Y. Kawaguchi and B. Sakaguchi 1989 Purification and characterization of novel chymotrypsin inhibitor controlled by the silkworm, Bombyx mori. Comp. Biochem. Physiol., 94B: 149-155
5. Fujii, H., H. Aratake, H. Doira and K. Koga 1996a Genetic analysis of chymotrypsin inhibitors in the hemolymph of Bombyx mori. J. Seric. Sci. Jpn., 65: 334-341
6. Fujii, H., H. Aratake and H. Doira 1996b Genetic analysis of hemolymph chymotrypsin inhibitor-3 and 4 in the silkworm, Bombyx mori. J. Seric. Sci. Jpn., 65: 385-389
7. Hayashi H. 1983 A filter paper technique for the micro detemination of protein. Seikagaku, 55: 257-258. (in Japanese)
8. He, N. J., M. Yakiyama, H. Fujii, Y. Banno and K. Yamamoto 2003 Genomic structure and expression analysis of the gene encoding a silkworm basic Kunitz-type chymotrypsin inhibitor. Biochem. Biophys. Acta., 1628: 71-77
9. Kurioka A, M. Yamazaki and H. Hirano 1999 Primary structure and possible functions of a trypsin inhibitor of Bombyx mori. Eur. J. Biochem., 259(1-2): 120-126
10. Laemmli, U, K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685
11. Narayanaswamy, R. S. Manickam and E. M. John 1988 Purification and characterization of two trypsin inhibitors from the hemolymph of Manduca sexta larvae. J. Biol. Chem., 263(23): 11523-11527
12. Sasaki, T., 1991, Patchwork-structure serpin from silkworm (Bombyx mori) larval hemolymph. Eur. J. Biochem., 202(2): 255-261
13. Sato, S. and S. Murao (1973): Isolation and crystallization of microbial alkaline protease inhibitor, S-SI. Argic. Bio. chem., 37: 1067-1074
14. Shinohara, T., Y. Aso, K. Shirai, H. Fujii and G. Funatsu 1993 Purification of chymotrypsin inhibitors from larval hemolymph of the silkworm, Bombyx mori. Biosci. Biotech. Biochem., 57: 1067-1071
15. Shirai, K., H. Fujii and H. Doira 1997 Purification and characterization of a chymotrypsin inhibitor with an oligosaccharide chain from the hemolymph of Bombyx mori. J. Seric. Sci. Jpn., 66: 253-260
16. Shirai, K., H. Fujii, H. Doira and H. Iwamoto 2000, Synthesis and resorption of a humoral chymotrypsin inhibitor, CI-8, by fat body of the silkworm, Bombyx mori. Insect Biochem and Molec. Bio., 30: 363-368
17. Taguchi, S., S. Kojima, M. Terabe, K. Miura and H. Momose 1994 Comparative studies on the primary structures and inhibitory properties of subtilisin-trypsin inhibitors from Streptomyces. Eur. J. Biochem., 220(3): 911-918
18. Yoshimoto, M. and M. Sr. Laskowski 1982 Large scale purification of isoinhibitors of trypsin from swine colostrum using zinc chelate chromatography and chromatofocusing. Preparative Biochem., 12: 235-254