Specificity inversion of Ochrobactrum anthropi D-aminopeptidase to a D,D-carboxypeptidase with new penicillin binding activity by directed mutagenesis

Protein Science

Volumn 14, Issue 9, 2005, Pages 2296-2303

  Delmarcelle, Michaël ^a   Boursoit, Marie Caroline ^a   Filée, Patrice ^a   Baurin, Stéphane Lucius ^a   Frère, Jean Marie ^a   Joris, Bernard ^a  

^a UNIVERSITY OF LIÈGE   (Belgium)

Author keywords

Directed evolution; Ochrobactrum anthropi D aminopeptidase; Penicillin binding proteins; Serine penicillin recognizing proteins; Streptomyces R61 D,D peptidase

Indexed keywords

AMINOPEPTIDASE; BACTERIAL ENZYME; CARBOXYPEPTIDASE; PENICILLIN BINDING PROTEIN;

ARTICLE; BINDING AFFINITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STRUCTURE; NONHUMAN; OCHROBACTRUM ANTHROPI; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FAMILY; SITE DIRECTED MUTAGENESIS;

AMINOPEPTIDASES; BINDING SITES; MUTAGENESIS, SITE-DIRECTED; OCHROBACTRUM ANTHROPI; PENICILLIN-BINDING PROTEINS; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SERINE-TYPE D-ALA-D-ALA CARBOXYPEPTIDASE; STREPTOMYCES; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

OCHROBACTRUM ANTHROPI; STREPTOMYCES;

EID: 24344454624     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.051475305     Document Type: Article

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