Paired helical filaments (PHFs) are a family of single filament structures with a common helical turn period: Negatively stained PHF imaged by TEM and measured before and after sonication, deglycosylation, and dephosphorylation

Microscopy Research and Technique

Volumn 67, Issue 3-4, 2005, Pages 175-195

  Ruben, George C ^a   Wang, Jian Zhi ^b   Iqbal, Khalid ^c   Grundke Iqbal, Inge ^c  

^a Dartmouth College   (United States)

^b HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^c NEW YORK STATE INSTITUTE FOR BASIC RESEARCH IN DEVELOPMENTAL DISABILITIES   (United States)

Author keywords

2.0 tau polymer like filaments; Cylindrical filaments (CF); Cylindrical filaments with periodic thin regions (CF PT)

Indexed keywords

FILM PREPARATION; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; PERIODIC STRUCTURES; SONICATION;

2.0 TAU POLYMER-LIKE FILAMENT; CYLINDRICAL FILAMENT; CYLINDRICAL FILAMENT WITH PERIODIC THIN REGION; DEGLYCOSYLATION; DEPHOSPHORYLATIONS; FILAMENT STRUCTURE; GLUTARALDEHYDES; HIGH-RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; PAIRED HELICAL FILAMENTS; SINGLE FILAMENTS;

CARBON FILMS;

CARBON; GLUTARALDEHYDE; N ACETYL BETA GLUCOSAMINIDASE; PHOSPHOTUNGSTIC ACID;

ALZHEIMER DISEASE; ARTICLE; CONTROLLED STUDY; DEGLYCOSYLATION; DEPHOSPHORYLATION; FILM; HUMAN; HUMAN TISSUE; IMAGE ANALYSIS; MOLECULAR SIZE; NEUROFIBRILLARY TANGLE; PAIRED HELICAL FILAMENT; PARAMETER; PH; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REACTION ANALYSIS; TRANSMISSION ELECTRON MICROSCOPY; ULTRASOUND; VAPOR;

EID: 24144499309     PISSN: 1059910X     EISSN: None     Source Type: Journal    
DOI: 10.1002/jemt.20197     Document Type: Article

References (31)

1. Alafuzoff I, Iqbal K, Friden H, Adolfsson R, Winblad B. 1987. Histopathological criteria for progressive dementia disorders: clinical-pathological correlation and classification by multivariate data analysis. Acta Neuropathol (Berl) 74:209-225.
2. Goedert M, Spillantini MG, Cairns NJ, Crowther RA. 1992. Tau proteins of Alzheimer paired helical filaments: abnormal phosphorylation of all six brain isoforms. Neuron 8:159-168.
3. Grundke-Iqbal I, Iqbal K, Quinlan M, Tung Y-C, Zaidi MS, Wisniewski HM. 1986a. Microtubule-associated protein tau: a component of Alzheimer paired helical filaments. J Biol Chem 261:6084-6089.
4. Grundke-Iqbal I, Iqbal K, Tung Y-C, Quinlan M, Zaidi MS, Wisniewski HM, Binder LI. 1986b. Abnormal phosphorylation of the microtubule associated protein tau in Alzheimer cytoskeletal pathology. Proc Natl Acad Sci U S A 83:4913-4917.
5. Grundke-Iqbal I, Vorbrodt AW, Iqbal K, Tung Y-C, Wang GP, Wisniewski HM. 1988. Microtubule-associated polypeptides tau are altered in Alzheimer paired helical filaments. Mol Brain Res 4:43-52.
6. Iqbal K, Zaidi T, Thompson CH, Merz PA, Wisniewski HM. 1984. Alzheimer paired helical filaments: bulk isolation, solubility and protein composition. Acta Neurpathol (Berl) 62:167-177.
7. Iqbal K, Grundke-Iqbal I, Smith AJ, George L, Tung Y-C, Zaidi T. 1989. Identification and localization of a tau peptide to paired helical filaments of Alzheimer disease. Proc Natl Acad Sci USA 86:5646-5650.
8. Kidd M. 1963. Paired helical filaments in electron microscopy of Alzheimer's disease. Nature 197:192-193.
9. Kidd M. 1964. Alzheimer's disease: an electron microscopical study. Brain 87:307-320.
10. Kopke E, Tung Y-C, Shaikh S, Alonso Adel C, Iqbal K, Grundke-Iqbal I. 1993. Microtubule associated protein tau: abnormal phosphorylation of a non-paired helical filament pool in Alzheimer's disease. J Biol Chem 268:24374-24384.
11. Moreno-Herrero F, Valpuesta JM, Perez M, Colchero J, Baro AM, Avila J, de Garcini EM. 2001. Characterization by atomic force microscopy and cryoelectron microscopy of tau polymers assembled in Alzheimer's disease. J Alzheimer's Dis 3:443-451.
12. Pollanen MS, Markiewicz P, Bergron C, Goh MC. 1994. Twisted ribbon structure of paired helical filaments revealed by atomic force microscopy. Am J Pathol 144:869-873.
13. Pollanen MS, Markiewicz P, Goh MC. 1997. Paired helical filaments are twisted ribbons composed of two parallel and aligned components: image reconstruction and modeling of filament structure using atomic force microscopy. J Neuropathol Exp Neurol 56:79-85.
14. Ruben GC, Iqbal K, Grundke-Iqbal I, Wisniewski HM, Ciardelli TL, Johnson Jr JE. 1991. The microtubule associated protein tau forms a triple-stranded left-hand helical polymer. J Biol Chem 266: 22019-22027.
15. Ruben GC, Iqbal K, Wisniewski HM, Johnson Jr JE, Grundke-Iqbal I. 1992. Alzheimer neurofibrillary tangles contain 2.1 nm filaments structurally identical to the microtubule associated protein tau: a high resolution transmission electron microscopy study of tangles and senile plaque core amyloid. Brain Res 590:164-179.
16. Ruben GC, Iqbal K, Grundke-Iqbal I, Johnson Jr JE. 1993. The organization of the microtubule associated protein tau in Alzheimer paired helical filaments. Brain Res 602:1-13.
17. Ruben GC, Novak M, Edwards PC, Iqbal K. 1995a. Alzheimer paired helical filaments, untreated and pronase digested, studied by vertical platinum-carbon replication and high resolution transmission electron microscopy. Brain Res 675:1-12.
18. Ruben GC, Iqbal K, Grundke-Iqbal I. 1995b. Helical ribbon morphology in neurofibrillary tangles of paired helical filaments. In: Iqbal K, Mortimer JA, Winblad B, Wisniewski HM, editors. Research advances in Alzheimer's disease and related disorders. New York: John Wiley & Sons, p 477-486.
19. Ruben GC, Ciardelli TL, Grundke-Iqbal I, Iqbal K. 1997. Alzheimer disease hyperphosphorylated tau aggregates hydrophobically. Synapse 27:208-229.
20. Ruben GC, Wang J-Z, Grundke-Iqbal I, Iqbal K. 1999. PHF have a wide range of widths with similar helical periods. In: 6th International Alzheimer's Meeting Proceedings. Chichester, UK: John Wiley & Sons, p 187-192.
21. Terry RD, Gonatas NK, Weiss M. 1964. Ultrastructural studies in Alzheimer's presenile dementia. Am J Pathol 44:269-297.
22. Tomlinson BE, Blessed G, Roth M. 1970. Observations on the brains of demented old people. J Neurol Sci 11:205-242.
23. Wang J-Z, Gong C-X, Zaidi T, Grundke-Iqbal I, Iqbal K. 1995. Dephosphorylation of Alzheimer paired helical filaments by protein phosphatase-2A and -2B. J Biol Chem 270:4854-4860.
24. Wang J-Z, Grundke-Iqbal I, Iqbal K. 1996. Glycosylation of microtubule-associated protein tau: an abnormal posttranslational modification in Alzheimer's disease. Nat Med 2:871-875.
25. Wen GY, Wisniewski HM. 1987. High resolution analysis of paired helical filaments in Alzheimer's disease. J Electron Microsc Tech 5:347-355.
26. Wilcock GK, Esiri MM. 1982. Plaques, tangles and dementia: a quantitative study. J Neurol Sci 56:343-356.
27. Wischik CM, Crowther RA, Stewart M, Roth M. 1985. Subunit structure of paired helical filaments in Alzheimer's disease. J Cell Biol 100:1905-1912.
28. Wisniewski HM, Wen GY. 1985. Structures of paired helical filaments from Alzheimer's disease neurofibrillary tangles. Acta Neuropathol 66:173-176.
29. Wisniewski HM, Narang HK, Terry RD. 1976. Neurofibrillary tangles of paired helical filaments. J Neurol Sci 27:173-181.
30. Wisniewski HM, Merz PA, Iqbal K. 1984. Ultrastructure of paired helical filaments of Alzheimer's neurofibrillary tangle. J Neuropathol Exp Neurol 43:643-656.
31. Wisniewski HM, Wen GY, Wang KC, Iqbal K, Rubenstein R. 1986. Determination of the handedness of paired helical filaments in Alzheimer disease. In: Metuzals J, editor. Electron microscopy and Alzheimer diseases. San Francisco: San Francisco Press, p 21-24.