Validation of use of purine bases as a microbial marker by 15N labelling in growing lambs given high-concentrate diets: Effects of grain processing, animal age and digesta sampling site

Animal Science

Volumn 81, Issue 1, 2005, Pages 57-65

  Askar, A R ^a,b   Guada, J A ^a   Balcells, J ^a   De Vega, A ^a   Castrillo, C ^a  

^a UNIVERSITY OF ZARAGOZA   (Spain)

^b Desert Research Center   (Egypt)

Author keywords

15N, purines; Barley; Lambs; Microbial markers

Indexed keywords

ANIMALIA; HORDEUM VULGARE SUBSP. VULGARE; OVIS ARIES;

EID: 24044520322     PISSN: 13577298     EISSN: None     Source Type: Journal    
DOI: 10.1079/ASC50390057     Document Type: Article

References (50)

1. Aharoni, Y. and Tagari, H. 1991. Use of nitrogen-15 determinations of purine fractions of digesta to define nitrogen metabolism traits in the rumen. Journal of Dairy Science 74: 2540-2547.
2. Al-Khalidi, U. A. S. and Chaglassian, T. H. 1965. The species distribution of xanthine oxidase. Biochemical Journal 97: 318-320.
3. Association of Official Analytical Chemists. 1984. Official methods of analysis, 13th edition. AOAC, Washington, DC.
4. Badawy, A. M., Campbell, R. M., Cuthbertson, D. P., Fell, B. F. and Mackie, W. S. 1958. Further studies on the changing composition of the digesta along the alimentary tract of the sheep. 1. Total and non-protein nitrogen. British Journal of Nutrition 12: 367-383.
5. Balcells, J., Guada, J. A., Peiro, J. M. and Parker, D. S. 1992. Simultaneous determination of allantoin and oxypurines in biological fluids by high performance liquid chromatography. Journal of Chromatography 575: 153-157.
6. Beauchemin, K. A., Yang, W. Z. and Rode, L. M. 2001. Effect of barley grain processing on the site and extent of digestion of beef feedlot finishing diets. Journal of Animal Science 79: 1925-1936.
7. Broderick, G. A. and Merchen, N. R. 1992. Markers for quantifying microbial protein synthesis in the rumen. Journal of Dairy Science 75: 2618-2632.
8. Calsamiglia, S., Stern, M. D. and Firkins, J. L. 1997. Comparison of nitrogen-15 and purines as microbial markers in continuous culture. Journal of Animal Science 74: 1375-1381.
9. 15N and purine bases) and bacterial isolates for the estimation of rumen microbial protein synthesis. Animal Science 75: 315-321.
10. Cecava, M. J., Merchen, N. R., Berger, L. L., Mackie, R. I. and Fahey Jr, G. C. 1991. Effects of dietary energy level and protein source on nutrient digestion and ruminal nitrogen metabolism in steers. Journal of Animal Science 69: 2230-2243.
11. Condon, R. J., Hall, G. and Hatfield, E. E. 1970. Metabolism of abomasally infused 14C labelled ribonucleic acid, adenine, uracil and glycine. Journal of Animal Science 31: 1037-1038.
12. Craig, M. W., Brown, D. R., Broderick, A. and Ricker, D. B. 1987. Post-prandial composition changes of fluid and particle-associated ruminal microorganism. Journal of Animal Science 65: 1024-1048.
13. 15N isotope dilution technique. Journal of Animal Science 68: 409-418.
14. Dewhurst, R. J., Hepper, D. and Webster, A. J. F. 1995. Comparison of in sacco and in vitro techniques for estimating the rate and extent of rumen fermentation of a range of dietary ingredients. Animal Feed Science and Technology 51: 211-229.
15. Djouvinov, D. S., Nakashima, Y., Todorov, N. and Pavlov, D. 1998. In situ degradation of feed purines. Animal Feed Science and Technology 71: 67-77.
16. Faichney, G. J. 1975. The use of markers to partition digestion within the gastrointestinal tract of ruminants. In Digestion and metabolism in the ruminant (ed. I. W. McDonald and A. C. I. Warner), pp. 277-291. University of New England Publishing Unit, Armidale, NSW, Australia.
17. Firkins, J. L., Lewis, S. M., Montgomery, L., Berger, L. L., Merchen, N. R. and Fahey Jr, G. C. 1987. Effects of feed intake and dietary urea concentration on ruminal dilution rate and efficiency of bacterial growth in steers. Journal of Dairy Science 70: 2312-2321.
18. Galyean, M. L., Wagner, D. G. and Owens, F. N. 1979. Corn particle size and site and extent of digestion by steers. Journal of Animal Science 49: 204-210.
19. Galyean, M. L., Wagner, D. G. and Owens, F. N. 1981. Dry matter and starch disappearance of corn and sorghum as influenced by particle size and processing. Journal of Dairy Science 64: 1804-1812.
20. Goering, H. K. and Van Soest, P. J. 1970. Forage fiber analysis. Agricultural handbook no. 379. Agricultural Research Service, US Department of Agriculture.
21. John, A. and Ulyatt, M. J. 1984. Measurement of protozoa using phosphatidyl choline and of bacteria using nucleic acids in the duodenal digesta of sheep fed chaffed lucerne hay (Medicago sativa L.) diets. Journal of Agricultural Science, Cambridge 102: 33-44.
22. Hvelplund, T. and Weisbjerg, M. R. 2000. In situ techniques for the estimation of protein degradability and postrumen availability. In Forage evaluation in ruminant nutrition (ed. D. I. Givens, E. Owens, R. F. E. Axford and H. M. Omed), pp. 233-258. CABI Publishing, Wallingford, UK.
23. McAllan, A. B. and Smith, R. H. 1973. Degradation of nucleic acids in the rumen. British Journal of Nutrition 29: 331-345.
24. Madsen, J. and Hvelplund, T. 1985. Protein degradation in the rumen. A comparison between in vivo, nylon bags, in vitro and buffer measurements. Acta Agriculturæ Scandinavica. 25: (suppl.) 103-124.
25. Martín-Orúe, S. M., Balcells, J., Guada, J. A. and Castrillo, C. 1995. Endogenous purine and pyrimide derivative excretion in pregnant sows. British Journal of Nutrition 73: 375-385.
26. Martín-Orúe, S. M., Balcells, J., Zakraoui, F. and Castrillo, C. 1998. Quantification and chemical composition of mixed bacteria harvested from solid fractions of rumen digesta: effect of detachment procedure. Animal Feed Science and Technology 71: 269-282.
27. Mayes, R. W., Lamb, C. S. and Colgrove, P. M. 1986. The use of dosed and herbage n-alkanes as markers for the determination of herbage intake. Journal of Agricultural Science, Cambridge 107: 161-170.
28. Mayes, R. W., Lamb, C. S. and Colgrove, P. M. 1988. Digestion and metabolism of dosed even-chain n-alkanes in sheep. Proceedings of the 12th general meeting of the European Grassland Federation, pp. 159-163.
29. 15N from urea to lysine in the human infant. British Journal of Nutrition 83: 505-512.
30. Nocek, J. E. and Tamminga, S. 1991. Site of digestion of starch in the gastrointestinal tract of dairy cows and its effect on milk yield and composition. Journal of Dairy Science 74: 3598-3629.
31. Offner, A., Bach, A. and Sauvant, D. 2003. Quantitative review of in situ degradation in the rumen. Animal Feed Science and Technology 106: 81-93.
32. Ohajuruka, O. A. and Palmquist, D. L. 1991. Evaluation of n-alkanes as digesta markers in dairy cows. Journal of Animal Science 69: 1726-1732.
33. Oliván, M. and Osoro, K. 1999. Effect of the temperature on alkane extraction from faeces and herbage. Journal of Agricultural Science, Cambridge 132: 305-312.
34. Ørskov, E. R., MacLeod, N. A. and Kyle, D. J. 1986. Flow of nitrogen from the rumen and abomasum in cattle and sheep given protein-free nutrients by intragastric infusion. British Journal of Nutrition 56: 241-248.
35. Owens, F. N. and Goetsch, A. L. 1986. Digesta passage and microbial protein synthesis. In Control of digestion and metabolism in ruminants (ed. L. P. Milligan, W. L. Grovum and A. Dobson), pp. 196-223. Prentice-Hall, Englewood Cliffs, NJ.
36. Pérez, J. F., Balcells, J., Guada, J. A. and Castrillo, C. 1997. Contribution of dietary nitrogen and purine bases to the duodenal digesta: comparison of duodenal and polyester-bag measurements. Animal Science 65: 237-245.
37. Pérez, J. F., Rodríguez, C., Gonzalez, J., Balcells, J. and Guada, J. A. 1996. Contribution of dietary purine bases to duodenal digesta in sheep. In situ studies of purine degradability corrected for microbial contamination. Animal Feed Science and Technology 62: 251-262.
38. Poncet, C., Ivan, M. and Leveille, M. 1982. Electromagnetic measurements of duodenal digesta flow in cannulated sheep. Reproduction, Nutrition, Développement 22: 651-660.
39. Ranilla, M. J. and Carro, M. D. 2003. Diet and procedures used to detach particle-associated microbes from ruminal digesta influence chemical composition of microbes and estimation of microbial growth in Rusitec fermenters. Journal of Animal Science 8: 537-544.
40. Razzaque, M. A., Topps, J. H., Kay, R. N. B. and Brockway, J. M. 1981. Metabolism of nucleic acids of rumen bacteria by preruminant and ruminant lambs. British Journal of Nutrition 45: 517-527.
41. Rodríguez, C. A., González, J., Alvir, M. R., Reppeto, J. L., Centeno, C. and Lamrani, F. 2000. Composition of bacteria harvested from the liquid and solid fractions of the rumen of sheep as influenced by feed intake. British Journal of Nutrition 84: 369-376.
42. Rodríguez, C. A., González, J., Alvir, M. R., Redondo, R. and Cajarville, C. 2003. Effects of feed intake on composition of sheep rumen contents and their microbial population size. British Journal of Nutrition 89: 97-103.
43. Smith, R. H., McAllan, A. B., Hewitt, D. and Lewis, P. E. 1978. Estimation of amounts of microbial and dietary nitrogen compounds entering the duodenum of cattle. Journal of Agricultural Science, Cambridge 90: 557-568.
44. Statistical Analysis Systems Institute. 2000. Statistical analysis systems, release 8. 01. Statistical Analysis Systems Institute Inc., Cary, NC.
45. Valiente, O. L., Delgado, P., Vega, A. de and Guada, J. A. 2003. Validation of the n-alkane technique to estimate intake, digestibility, and diet composition in sheep consuming mixed grain: roughage diets. Australian Journal of Agricultural Research 54: 693-702.
46. Van Soest, P. J., Robertson, J. B. and Lewis, B. A. 1991. Methods for dietary fiber, neutral detergent fiber, and non starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74: 3583-3597.
47. 15N) of different microbial fractions. Animal Science 78: 159-167.
48. Wenham, G. and Wyburn, R. S. 1980. A radiological investigation of the effects of cannulation on intestinal motility and digesta flow in sheep. Journal of Agricultural Science, Cambridge 95: 539-546.
49. Yang, W. Z., Beauchemin, K. A. and Rody, L. M. 2001. Effect of dietary factors on distribution and chemical composition of liquid-or solid-associated bacterial populations in the rumen of dairy cow. Journal of Animal Science 79: 2736-2746.
50. Zinn, R. A. and Owens, F. N. 1986. A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis. Canadian Journal of Animal Science 66: 157-166.