Desalting minimal amounts of DNA for electroporation in E. coli: A comparison of different physical methods

Biotechnology Letters

Volumn 27, Issue 14, 2005, Pages 1003-1005

  Schlaak, Christina ^a   Hoffmann, Peter ^a   May, Karin ^a   Weimann, Andreas ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Desalting; Dialysis; Electroporation; Gel filtration; Microcolumn

Indexed keywords

CLONING; DIALYSIS; ESCHERICHIA COLI; ETHANOL; FILTRATION; PRECIPITATION (CHEMICAL); PURIFICATION; SALT REMOVAL;

ELECTROPORATION; GEL FILTRATION; MICROCOLUMNS; MOLECULAR CLONING;

DNA;

BACTERIAL DNA;

ARTICLE; BACTERIUM TRANSFORMATION; CHEMISTRY; COMPARATIVE STUDY; ELECTROPORATION; ESCHERICHIA COLI; GENETICS; PLASMID;

DNA, BACTERIAL; ELECTROPORATION; ESCHERICHIA COLI; PLASMIDS; TRANSFORMATION, BACTERIAL;

ESCHERICHIA COLI;

EID: 24044493927     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-005-7867-z     Document Type: Article

References (4)

1. WJ Dower JF Miller CW Ragsdale 1988 High efficiency transformation of E. coli by high voltage electroporation Nucleic Acid Res 16 6127 6145
2. JF Miller WJ Dower CW Ragsdale 1988 High voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA Proc. Natl. Acad. Sci. USA 85 856 860
3. J Sambrook EF Fritsch T Maniatis 1989 Molecular Cloning: A Laboratory Manual 2 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY
4. TJ Silhavy ML Berman LW Enquist 1984 Experiments with Gene Fusion Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY