A microfluidic chemostat for experiments with bacterial and yeast cells

Nature Methods

Volumn 2, Issue 9, 2005, Pages 685-689

  Groisman, Alex ^a   Lobo, Caroline ^b   Cho, HoJung ^b   Campbell, J Kyle ^a   Dufour, Yan S ^c   Stevens, Ann M ^c   Levchenko, Andre ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b Johns Hopkins University   (United States)

^c VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELASTOMER;

ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIUM COLONY; CELL CULTURE; CELL DENSITY; CELL PROLIFERATION; CHEMOSTAT; CONTROLLED STUDY; CULTURE MEDIUM; DIFFUSION; FUNGUS GROWTH; MATHEMATICAL COMPUTING; MEMBRANE PERMEABILITY; METABOLITE; MICROFLUIDICS; MICROSCOPY; NONHUMAN; NUTRIENT LIMITATION; PRIORITY JOURNAL; TEMPERATURE DEPENDENCE; YEAST CELL;

BIOREACTORS; CELL SEPARATION; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; ESCHERICHIA COLI; FLOW CYTOMETRY; FLOW INJECTION ANALYSIS; IMAGE CYTOMETRY; MICROFLUIDIC ANALYTICAL TECHNIQUES; SACCHAROMYCES CEREVISIAE;

FUNGI;

EID: 24044453695     PISSN: 15487091     EISSN: None     Source Type: Journal    
DOI: 10.1038/nmeth784     Document Type: Article

References (30)

1. Stoodley, P., Sauer, K., Davies, D.G. & Costerton, J.X. Biofilms as complex differentiated communities. Annu. Rev. Microbiol. 56, 187-209 (2002).
2. Reynolds, T.B. & Fink, G.R. Bakers' yeast, a model for fungal biofilm formation. Science 291, 878-881 (2001).
3. Douglas, L.J. Candida biofilms and their role in infection. Trends Microbiol. 11, 30-36 (2003).
4. Anderson, G.G. et al. Intracellular bacterial biofilm-like pods in urinary tract infections. Science 301, 105-107 (2003).
5. Jelsbak, L. & Sogaard-Andersen, L. Pattern formation: fruiting body morphogenesis in Myxococcus xanthus. Curr. Opin. Microbiol. 3, 637-642 (2000).
6. Udvardi, M.K. & Day, D.A. Metabolite transport across symbiotic membranes of legume nodules. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48, 493-523 (1997).
7. Tebo, B.M., Linthicum, D.S. & Nealson, K.H. Luminous bacteria and light emitting fish: ultrastructure of the symbiosis. Biosystems 11, 269-280 (1979).
8. Scherz, R., Shinder, V. & Engelberg, D. Anatomical analysis of Saccharomyces cerevisiae stalk-like structures reveals spatial organization and cell specialization. J. Bacteriol. 183, 5402-5413 (2001).
9. Hastings, J.W. & Nealson, K.H. Bacterial bioluminescence. Annu. Rev. Microbiol. 31, 549-595 (1977).
10. Sternberg, C. et al. Distribution of bacterial growth activity in flow-chamber biofilms. Appl. Environ. Microbiol. 65, 4108-4117 (1999).
11. Millar, M.R., Linton, C.J. & Sherriff, A. Use of a continuous culture system linked to a modified Robbins device or flow cell to study attachment of bacteria to surfaces. Methods Enzymol. 337, 43-62 (2001).
12. Li, N., Tourovskaia, A. & Folch, A. Biology on a chip: microfabrication for studying the behavior of cultured cells. Critical Reviews in Biomedical Engineering 31, 423-488 (2003).
13. Andersson, H. & van den Berg, A. Microfluidic devices for cellomics: a review. Sens. Actuators B Chem. 92, 315-325 (2003).
14. Sia, S.K. & Whitesides, G.M. Microfluidic devices fabricated in poly(dimethylsiloxane) for biological studies. Electrophoresis 24, 3563-3576 (2003).
15. Mao, H.B., Cremer, P.S. & Manson, M.D. A sensitive, versatile microfluidic assay for bacterial chemotaxis. Proc. Natl. Acad. Sci. USA 100, 5449-5454 (2003).
16. Balaban, N.Q., Merrin, J., Chait, R., Kowalik, L. & Leibler, S. Bacterial persistence as a phenotypic switch. Science 305, 1622-1625 (2004).
17. Park, S. et al. Influence of topology on bacterial social interaction. Proc. Natl. Acad. Sci. USA 100, 13910-13915 (2003).
18. Mao, H.B., Yang, T.L. & Cremer, P.S. A microfluidic device with a linear temperature gradient for parallel and combinatorial measurements. JACS 124 (2002).
19. Unger, M.A., Chou, H.P., Thorsen, T., Scherer, A. & Quake, S.R. Monolithic microfabricated valves and pumps by multilayer soft lithography. Science 288, 113-116 (2000).
20. Miller, M.B. & Bassler, B.L. Quorum sensing in bacteria. Annu. Rev. Microbiol. 55, 165-199 (2001).
21. Fuqua, C., Parsek, M.R. & Greenberg, E.P. Regulation of gene expression by cell-to-cell communication: acyl-homoserine lactone quorum sensing. Annu. Rev. Genet. 35, 439-468 (2001).
22. Withers, H., Swift, S. & Williams, P. Quorum sensing as an integral component of gene regulatory networks in gram-negative bacteria. Curr. Opin. Microbiol. 4, 186-193 (2001).
23. Andersen, J.B. et al. gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication. Appl. Environ. Microbiol. 67, 575-585 (2001).
24. Elowitz, M.B., Levine, A.J., Siggia, E.D. & Swain, P.S. Stochastic gene expression in a single cell. Science 297, 1183-1186 (2002).
25. Ozbudak, E.M., Thattai, M., Kurtser, I., Grossman, A.D. & van Oudenaarden, A. Regulation of noise in the expression of a single gene. Nat. Genet. 31, 69-73 (2002).
26. Blake, W.J., Kaern, M., Cantor, C.R. & Collins, J.J. Noise in eukaryotic gene expression. Nature 422, 633-637 (2003).
27. McMurray, M.A. & Gottschling, D.E. An age-induced switch to a hyper-recombinational state. Science 301, 1908-1911 (2003).
28. Flardh, K. Growth polarity and cell division in Streptomyces. Curr. Opin. Microbiol. 6, 564-571 (2003).
29. Xia, Y.N. & Whitesides, G.M. Soft lithography. Angew. Chem. Int. Ed. 37, 551-575 (1998).
30. Groisman, A., Enzelberger, M. & Quake, S.R. Microfluidic memory and control devices. Science 300, 955-958 (2003).