LaNe RAGE: A new tool for genomic DNA flanking sequence determination

Electronic Journal of Biotechnology

Volumn 8, Issue 2, 2005, Pages 218-225

  Park, Daniel J ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Cloning; DNA fingerprinting; Genomic DNA walking; PCR; Sequencing; Transposon mutagenesis

Indexed keywords

CLONING; ENZYMES; GENES;

DNA FINGERPRINTING; FLANKING SEQUENCE; GENOMIC DNA WALKING; PCR; SEQUENCING; TRANSPOSON MUTAGENESIS;

DNA;

ARTICLE; DNA DETERMINATION; DNA FINGERPRINTING; DNA FLANKING REGION; DNA SEQUENCE; GENE AMPLIFICATION; GENE MAPPING; LARIAT DEPENDENT NESTED POLYMERASE CHAIN REACTION FOR RAPID AMPLIFICATION OF GENOMIC DNA ENDS; MOUSE; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; TRANSPOSON;

EID: 24044445549     PISSN: 07173458     EISSN: None     Source Type: Journal    
DOI: 10.2225/vol8-issue2-fulltext-7     Document Type: Article

References (5)

1. ARNOLD, C. and HODGSON, I.J. Vectorette PCR: a novel approach to genomic walking. PCR Methods and Applications, 1991, vol. 1, no. 1, p. 39-42.
2. DEVON, R.S.; PORTEOUS, D.J. and BROOKES, A.J. Splinkerettes - improved vectorettes for greater efficiency in PCR walking. Nucleic Acids Research, May 1995, vol. 23, no. 9, p. 1644-1645.
3. MUELLER, Paul R. and WOLD, Barbara. In vivo footprinting of a muscle specific enhancer by ligation mediated PCR. Science, November 1989, vol. 246, no. 4931, p. 780-786.
4. MYRICK, Kyl V. and GELBART, William M. Universal fast walking for direct and versatile determination of flanking sequence. Gene, February 2002, vol. 284, p. 125-131.
5. SARKAR, G.; TURNER, R.T. and BOLANDER, M.E. Restriction site PCR: a direct method of unknown sequence retrieval adjacent to a known locus by using universal primers. PCR Methods and Applications, 1993, vol. 2, no. 4, p. 318-322.