Preparation and identification of monoclonal antibodies against pea albumin 1b (PA1b)

Hybridoma

Volumn 24, Issue 4, 2005, Pages 197-200

  Wang, Jian He ^a   Dun, Xin Peng ^a   Qu, Li Na ^b   Zhao, Yan Ying ^a   Yang, Tang Bin ^b   Chen, Zheng Wang ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b INSTITUTE OF SPACE MEDICO ENGINEERING   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ALBUMIN; CARRIER PROTEIN; GLUTARALDEHYDE; MONOCLONAL ANTIBODY; PEA ALBUMIN 1B; UNCLASSIFIED DRUG;

ANTIBODY AFFINITY; ANTIBODY PRODUCTION; ANTIBODY TITER; ANTIGEN DETECTION; ARTICLE; CELL LINE; CELL PROLIFERATION; COMPETITIVE INHIBITION; ENZYME LINKED IMMUNOSORBENT ASSAY; HYBRIDOMA CELL CULTURE; LIGHT CHAIN; NONHUMAN; PEA; PEPTIDE ANALYSIS; PLANT SEED; PRIORITY JOURNAL; PROTEIN FUNCTION; THERMOSTABILITY;

PISUM SATIVUM;

EID: 23944485397     PISSN: 15540014     EISSN: None     Source Type: Journal    
DOI: 10.1089/hyb.2005.24.197     Document Type: Article

References (10)

1. Higgins TJ, Chandler PM, Randall PJ, et al: Gene structure, protein structure, and regulation of the synthesis of a sulfur-rich protein in pea seeds. J Biol Chem 1986;261:11124-11130.
2. Watanabe Y, Barbashov SF, Komatsu S, Hemmings AM, Miyagi M, Tsunasawa S, and Hirano H. A peplide thai stimulates phosphorylation of the plant insulin-binding protein. Eur J Biochem 1994;224:167-172.
3. Hancock KR, Ealing PM, and White DW. Identification of sulfur-rich proteins which resist rumen degradation and are hydrolysed rapidly by intestinal proteases. Br J Nutr 1994;72:855-863.
4. Spencer D, Higgins TJV, Freer M, Dove H, and Coombe JB. Monitoring the fate of dietary proteins in rumen fluid using gel electrophoresis. Br J Nutr 1988;60:241-247.
5. Louis S, Delobel B, Gressent F, Rahioui I, Quillian L, Vallier A, and Rahbe Y: Molecular and biological screening for insect-toxic seed albumins from four legume species. Plant Sci 2004;167: 705-714.
6. Delobel B, Grenier AM, Gue'guen J, Ferrasson E, and Mbaiguinam M: Utilisation d'un polypeptide de'rive' d'une albumine PA1b de le'gumineuse comme insecticide. French patent (1998) 9805877.
7. 1H-2-D NMR study and molecular modeling. Bio-chemistry 2003;42:11915-11923.
8. Jennings C, West J, Waine C, Craik D, and Anderson M: Biosynthesis and insecticidal properties of plant cyclotides: the cyclic knotted proteins from Oldenlandia affinis. Proc Natl Acad Sci USA 2001;98:10614-10619.
9. Crevieu I, Berot S, and Gueguen J: Large-scale procedure for fractionation of albumins and globulins from pea seeds. Nahrung 1996;40:237-244.
10. Yang TB, Zhong P, Nie JL, Li JS, Qu LN, Li YH, and Kan GH: Preparation and identification of specific and high-affinity monoclonal antibodies against PA1b. Hybridoma 2002;21:197-201.