Modified cell ELISA to determine the solubilization of cell surface proteins: Applications in GPI-anchored protein purification

Journal of Biochemical and Biophysical Methods

Volumn 64, Issue 2, 2005, Pages 99-109

  Bumgarner, Gary W ^a,c   Zampell, Jamie C ^b   Nagarajan, Shanmugam ^b   Poloso, Neil J ^b   Dorn, Amanda S ^b   D'Souza, Martin J ^a   Selvaraj, Periasamy ^b  

^a MERCER UNIVERSITY   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c SAMFORD UNIVERSITY   (United States)

Author keywords

B7 1; CD32A; Glycosylphosphatidyl inositol; ICAM 1; Protein purification; Solubility

Indexed keywords

B7 ANTIGEN; CELL SURFACE PROTEIN; GLUCOSIDE; GLYCOSYLPHOSPHATIDYLINOSITOL; INTERCELLULAR ADHESION MOLECULE 1; PROTEINASE; RECOMBINANT PROTEIN; SAPONIN; TRITON X 100;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME LINKED IMMUNOSORBENT ASSAY; GEL ELECTROPHORESIS; IMMUNOBLOTTING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; RADIOACTIVITY; RAT; SOLUBILIZATION;

ANIMALS; ANTIGENS, CD; ANTIGENS, CD80; CELL LINE, TUMOR; CELL MEMBRANE; DETERGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; GLYCOSYLPHOSPHATIDYLINOSITOLS; HYBRIDOMAS; IMMUNOBLOTTING; MACROMOLECULAR SUBSTANCES; OCTOXYNOL; RECEPTORS, IGG; SOLUBILITY; TEMPERATURE; TRANSFECTION;

EID: 23844541406     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbbm.2005.05.007     Document Type: Article

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