Pituitary homeobox 1 (Pitx1) stimulates rat LHβ gene expression via two functional DNA-regulatory regions

Journal of Molecular Endocrinology

Volumn 35, Issue 1, 2005, Pages 145-158

  Jiang, Qiaorong ^c   Jeong, Kyeong Hoon ^a   Horton, Cheryl D ^b   Halvorson, Lisa M ^c  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

^b TUFTS MEDICAL CENTER   (United States)

^c UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CIS ACTING ELEMENT; DNA; EARLY GROWTH RESPONSE FACTOR 1; LUTEINIZING HORMONE BETA SUBUNIT; STEROIDOGENIC FACTOR 1; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR PITX1; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; GEL MOBILITY SHIFT ASSAY; GENE CONSTRUCT; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE MUTATION; GENETIC TRANSFECTION; MOLECULAR MECHANICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN FUNCTION; REGULATORY DNA SEQUENCE;

ANIMALS; BASE SEQUENCE; BINDING SITES; CELL LINE; DNA; DNA-BINDING PROTEINS; EARLY GROWTH RESPONSE PROTEIN 1; GENE EXPRESSION; HOMEODOMAIN PROTEINS; IMMEDIATE-EARLY PROTEINS; KINETICS; LUTEINIZING HORMONE, BETA SUBUNIT; MUTAGENESIS, SITE-DIRECTED; PAIRED BOX TRANSCRIPTION FACTORS; PITUITARY GLAND; PROMOTER REGIONS (GENETICS); RATS; RECEPTORS, CYTOPLASMIC AND NUCLEAR; SEQUENCE DELETION; TRANSCRIPTION FACTORS; TRANSFECTION;

EID: 23844525678     PISSN: 09525041     EISSN: None     Source Type: Journal    
DOI: 10.1677/jme.1.01754     Document Type: Article

References (33)

1. deWet JR, Wood KV, DeLuca M, Helinski DR & Subramani S 1987 Firefly luciferase gene: structure and expression in mammalian cells. Molecular and Cellular Biology 7 725-737.
2. Dorn C, Ou Q, Svaren J, Crawford PA & Sadovsky Y 1999 Activation of luteinizing hormone beta gene by gonadotropin-releasing hormone requires the synergy of early growth response-1 and steroidogenic factor-1. Journal of Biological Chemistry 274 13870-13876.
3. Drouin J, Lamolet B, Lamonerie T, Lanctot C & Tremblay JJ 1998 The PTX family of homeodomain transcription factors during pituitary developments. Molecular and Cellular Endocrinology 140 31-36.
4. Edlund T, Walker MD, Barr PJ & Rutter WJ 1985 Cell specific expression of the rat insulin gene: evidence for a role of two distinct 5′ flanking elements. Science 230 912-916.
5. Halvorson LM, Kaiser UB & Chin WW 1996 Stimulation of luteinizing hormone beta gene promoter activity by the orphan nuclear receptor, steroidogenic factor-1. Journal of Biological Chemistry 271 6645-6650.
6. Halvorson LM, Ito M, Jameson JL & Chin WW 1998 Steroidogenic factor-1 and early growth response protein 1 act through two composite DNA binding sites to regulate luteinizing hormone beta-subunit gene expression. Journal of Biological Chemistry 273 14712-14720.
7. Jeong KH, Chin WW & Kaiser UB 2004 Essential role of the homeodomain for pituitary homeobox 1 activation of mouse gonadotropin-releasing hormone receptor gene expression through interactions with c-Jun and DNA. Molecular and Cellular Biology 24 6127-6139.
8. Kaiser UB, Sabbagh E, Chen MT, Chin WW & Saunders BD 1998 Sp1 binds to the rat luteinizing hormone beta (LHbeta) gene promoter and mediates gonadotropin-releasing hormone-stimulated expression of the LHbeta subunit gene. Journal of Biological Chemistry 273 12943-12951.
9. Kaiser UB, Halvorson LM & Chen MT 2000 Sp1, steroidogenic factor 1 (SF-1), and early growth response protein 1 (egr-1) binding sites form a tripartite gonadotropin-releasing hormone response element in the rat luteinizing hormone-beta gene promoter: an integral role for SF-1. Molecular Endocrinology 14 1235-1245.
10. Keri RA & Nilson JH 1996 A steroidogenic factor-1 binding site is required for activity of the luteinizing hormone beta subunit promoter in gonadotropes of transgenic mice. Journal of Biological Chemistry 271 10782-10785.
11. Keri RA, Bachmann DJ, Behrooz A, Herr BD, Ameduri RK, Quirk CC & Nilson JH 2000 An NF-Y binding site is important for basal, but not gonadotropin-releasing hormone-stimulated, expression of the luteinizing hormone beta subunit gene. Journal of Biological Chemistry 275 13082-13088.
12. Kurotani R, Tahara S, Sanno N, Teramoto A, Mellon PL, Inoue K, Yoshimura S & Osamura RY 1999 Expression of Pitx1 in the adult rat pituitary glands and pituitary cell lines: hormone-secreting cells and folliculo-stellate cells. Cell and Tissue Research 298 55-61.
13. Lala DS, Rice DA & Parker KL 1992 Steroidogenic factor I, a key regulator of steroidogenic enzyme expression, is the mouse homolog of fushi tarazu-factor I. Molecular Endocrinology 6 1249-1258.
14. Lamonerie T, Tremblay JJ, Lanctot C, Therrien M, Gauthier Y & Drouin J 1996 Pitx1, a bicoid-related homeo box transcription factor involved in transcription of the pro-opriomelanocortin gene. Genes and Development 10 1284-1295.
15. Lanctot C, Lamolet B & Drouin J 1997 The bicoid-related homeoprotein Pitx1 defines the most anterior domain of the embryo and differentiates posterior from anterior lateral mesoderm. Development 124 2807-2817.
16. Lanctot C, Gauthier Y & Drouin J 1999 Pituitary homeobox 1 (Pitx1) is differentially expressed during pituitary development. Endocrinology 140 1416-1422.
17. Melamed P, Koh M, Preklathan P, Bei L & Hew C 2002 Multiple mechanisms for Pitx-1 transactivation of a luteinizing hormone beta subunit gene. Journal of Biological Chemistry 277 26200-26207.
18. Nordeen S 1988 Luciferase reporter gene vectors for analysis of promoters and enhancers. Biotechniques 6 454-458.
19. Parker KL, Ikeda Y & Luo X 1996 The roles of steroidogenic factor-1 in reproductive function. Steroids 61 161-165.
20. Quentien MH, Manfroid I, Moncet D, Gunz G, Muller M, Grino M, Enjalbert A & Pellegrini I 2002 Pitx factors are involved in basal and hormone-regulated activity of the human prolactin promoter. Journal of Biological Chemistry 277 44408-44416.
21. Quirk CC, Lozada KL, Keri RA & Nilson JH 2001 A single Pitx1 binding site is essential for activity of the LHbeta promoter in transgenic mice. Molecular Endocrinology 15 734-746.
22. Rosenberg SB & Mellon PL 2002 An Otx-related homeodomain protein binds an LHbeta promoter element important for activation during gonadotrope maturation. Molecular Endocrinology 16 1280-1298.
23. Sambrook J, Fritsch EF & Maniatis T 1989 Molecular Cloning: a Laboratory Manual. New York: Cold Spring Harbor Press.
24. Szeto DP, Rodriguez-Esteban C, Ryan AK, O'Connell SM, Liu F, Kioussi C, Gleiberman AS, Izpisua-Belmonte JC & Rosenfeld MG 1999 Role of the Bicoid-related homeodomain factor Pitx1 in specifying hindlimb morphogenesis and pituitary development. Genes and Development 13 484-494.
25. Thomas P, Mellon PL, Turgeon J & Waring DW 1996 The L beta T2 clonal gonadotrope: a model for single cell studies of endocrine cell secretion. Endocrinology 137 2979-2989.
26. Tremblay JJ & Drouin J 1999 Egr-1 is a downstream effector of GnRH and synergizes by direct interaction with Pitx1 and SF-1 to enhance luteinizing hormone beta gene transcription. Molecular and Cellular Biology 19 2567-2576.
27. Tremblay JJ, Lanctot C & Drouin J 1998 The pan-pituitary activator of transcription, Pitx1 (pituitary homeobox 1), acts in synergy with SF-1 and Pit1 and is an upstream regulator of the Lim-homeodomain gene Lim3/Lhx3. Molecular Endocrinology 12 428-441.
28. Tremblay JJ, Marcil A, Gauthier Y & Drouin J 1999 Pitx1 regulates SF-1 activity by an interaction that mimics the role of the ligand-binding domain. EMBO Journal 18 3431-3441.
29. Windle JJ, Weiner RI & Mellon PL 1990 Cell lines of the pituitary gonadotrope lineage derived by targeted oncogenesis in transgenic mice. Molecular Endocrinology 4 597-603.
30. Wolfe MW 1999 The equine luteinizing hormone beta-subunit promoter contains two functional steroidogenic factor-1 response elements. Molecular Endocrinology 13 1497-1510.
31. Wolfe MW & Call GB 1999 Early growth response protein 1 binds to the luteinizing hormone-beta promoter and mediates gonadotropin-releasing hormone-stimulated gene expression. Molecular Endocrinology 13 752-763.
32. Zakaria MM, Jeong KH, Lacza C & Kaiser UB 2002 Pituitary homeobox 1 activates the rat FSHbeta (rFSHbeta) gene through both direct and indirect interactions with the rFSHbeta gene promoter. Molecular Endocrinology 16 1840-1852.
33. Zhao L, Bakke M & Parker KL 2001 Pituitary-specific knockout of steroidogenic factor 1. Molecular and Cellular Endocrinology 185 27-32.