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2] was synthesized according to [13].
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-1 from a Milli-Q water purification system directed onto the surface with a squeeze bottle. The samples were blow dried with compressed argon over silica gel and then imaged in the AFM. (b) Gel electrophoresis of drug-pBR322 complexes: pBR322 DNA aliquots (0.25 μg/mL) were incubated in the presence of the compounds in TE buffer at molar ration ri = 0.50 for electrophoresis study. Incubation was carried out in the dark at 37°C for 24 h; 24 mL aliquots of complex-DNA compounds containing 0.5 μg/DNA underwent 1% agarose gel electrophoresis for 4 h at 2 V/cm in 0.5XTBE (45 mM Tris-borate, 1 mM EDTA pH 8.0) buffer. Gel was subsequently stained in the same buffer containing ethidium bromide (1 mg/mL). The gel was photographed with an image Master® VDS, Pharmacia Biotech.
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