Development of a novel ampholyte buffer for isoelectric focusing: Electric charge-separation of protein samples for X-ray crystallography using free-flow isoelectric focusing

Acta Crystallographica Section D: Biological Crystallography

Volumn 61, Issue 6, 2005, Pages 799-802

  Kim, Seong Hoon ^a   Miyatake, Hideyuki ^a   Ueno, Tomoyuki ^b   Nagao, Takuya ^b   Miki, Kunio ^a,c  

^a RIKEN Harima Institute Research   (Japan)

^b TECAN JAPAN Co Ltd   (Japan)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMPHOLYTE; PROTEIN;

ARTICLE; CHEMISTRY; INSTRUMENTATION; ISOELECTRIC FOCUSING; ISOLATION AND PURIFICATION; METHODOLOGY; PH; X RAY CRYSTALLOGRAPHY;

AMPHOLYTE MIXTURES; CRYSTALLOGRAPHY, X-RAY; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; PROTEINS;

EID: 23844512823     PISSN: 09074449     EISSN: 13990047     Source Type: Journal    
DOI: 10.1107/S0907444905008085     Document Type: Conference Paper

References (17)

1. Balmann, H., Burgaud, C. & Sanchez, V. (1991). Sep. Sci. Technol. 26, 1481-1494.
2. Balmann, H. & Sanchez, V. (1991). Sep. Sci. Technol. 26, 127-132.
3. Baygants, J. C., Schwarz, B. C. E., Deshnukh, R. R. & Bier, M. (1997). J. Chromatogr. 779, 165-183.
4. Bier, M., Twitty, G. E. & Sloan, J. E. (1989). J. Chromatogr. 470, 369-376.
5. Burggraf, D., Weber, G. & Lottspeich, F. (1995). Electrophoresis, 16, 1010-1015.
6. Egen, N. B., Thormann, W., Twitty, G. E. & Bier, M. (1984). Electrophoresis, 83, 547-550.
7. Hanning, K. (1961). Z. Anal. Chem. 181, 244-254.
8. Kasicka, V., Prusik, Z., Smekal, O., Hlavacek, J., Barth, T., Weber, G. & Wagner, H. (1994). J. Chromatogr. B, 656, 99-106.
9. Kuhn, R., Hoffstetter-Kuhn, S. & Wagner, H. (1990). Electrophoresis, 11, 942-947.
10. Righetti, P. G., Barzaghi, B., Luzenna, M., Manfredi, G. & Faupel, M. (1987) J. Biochem. Biophys. Methods, 15, 199-206.
11. Righetti, P. G. & Gelfi, C. (1984). J. Biochem. Biophys. Methods, 9, 103-119.
12. Righetti, P. G., Wenisch, E. & Faupel, M. (1989). J. Chromatogr. 475, 293-309.
13. Svensson, H. (1962). Acta Chem. Scand. 16, 456-466.
14. Weber, G. & Bauer, J. (1998). Electrophoresis, 19, 1104-1109.
15. Weber, G. & Bocek, P. (1996). Electrophoresis, 17, 1906-1910.
16. Wenger, P., de Zuanni, M., Javet, P., Gelfi, C. & Righetti, P. G. (1987). J. Biochem. Biophys. Methods, 14, 29-43.
17. Yonemoto, T., Shirai, W. & Tadaki, T. (1995). Bioseparation, 5, 281-287.