Preparative centrifugal precipitation chromatography using dialysis membrane inserted into convoluted tubing

Journal of Liquid Chromatography and Related Technologies

Volumn 28, Issue 12-13, 2005, Pages 2061-2070

  Ng, Victor ^a   Yu, Henry ^b   Ito, Yoichiro ^c  

^a UNIVERSITY OF MARYLAND BALTIMORE COUNTY   (United States)

^b EMORY UNIVERSITY   (United States)

^c NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

Ammonium sulfate gradient precipitation; Centrifugal precipitation chromatography; Convoluted tubing; Dialysis tubing; Preparative separation; Protein purification and separation

Indexed keywords

AMMONIUM COMPOUNDS; CONCENTRATION (PROCESS); DIALYSIS MEMBRANES; DISSOLUTION; PHYSIOLOGICAL MODELS; PLASTIC PRODUCTS; PRECIPITATION (CHEMICAL); PROTEINS; PURIFICATION; SEPARATION; TUBING;

AMMONIUM SULFATE GRADIENT PRECIPITATION; CENTRIFUGAL PRECIPITATION CHROMATOGRAPHY; CONVOLUTED TUBING; DIALYSIS TUBING; PREPARATIVE SEPARATION; PROTEIN PURIFICATION AND SEPARATION;

CHROMATOGRAPHY;

AMMONIUM SULFATE; PLASMA PROTEIN;

ANALYTIC METHOD; CENTRIFUGAL PRECIPITATION CHROMATOGRAPHY; CHROMATOGRAPHY; CONFERENCE PAPER; DIALYSIS MEMBRANE; HUMAN; HUMAN TISSUE; HYPERBARISM; INSTRUMENTATION; PLASMA; PROTEIN ANALYSIS; SERUM; SOLUBILITY;

EID: 23744511574     PISSN: 10826076     EISSN: None     Source Type: Journal    
DOI: 10.1081/JLC-200063675     Document Type: Conference Paper

References (4)

1. Ito, Y. Centrifugal Precipitation Chromatography Applied to Fractionation of Proteins with Ammonium Sulfate. J. Liquid Chromatogr. & Rel. Technol. 1999, 22, 2825-2836.
2. Ito, Y. Centrifugal Precipitation Chromatography: Principle, Apparatus, and Optimization of Key Parameters for Protein Fractionation by Ammonium Sulfate Precipitation. Anal. Biochem. 2000, 277, 143-153.
3. Sookkumnerd, T.; Hsu, J.T.; Ito, Y. Gradient Formation in Membrane Unit for Differential Precipitation of Proteins. AIChE J. 2000, 46, 1191-1200.
4. Yu, H.; Ito, Y. Preparative Separation of Proteins Using Centrifugal Precipitation Chromatography Based on Solubility in Ammonium Sulfate Solution. Prep. Biochem. & Biotechnol. 2004, 34, 1-12.