Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus

Journal of Clinical Microbiology

Volumn 43, Issue 8, 2005, Pages 4262-4265

  Inoue, Masafumi ^a   Barkham, Timothy ^b   Keong, Lee Kok ^a   Gee, Lim Seng ^a   Wanjin, Hong ^a  

^a INSTITUTE OF MOLECULAR AND CELL BIOLOGY   (Singapore)

^b TAN TOCK SENG HOSPITAL   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; EPIDEMIC; INTERMETHOD COMPARISON; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SARS CORONAVIRUS; SENSITIVITY ANALYSIS; SEVERE ACUTE RESPIRATORY SYNDROME; SINGAPORE; VIRUS DETECTION; VIRUS GENOME;

HUMANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SARS VIRUS; SENSITIVITY AND SPECIFICITY;

CORONAVIRUS; SARS CORONAVIRUS;

EID: 23744489447     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.43.8.4262-4265.2005     Document Type: Article

References (19)

1. Adachi, D., G. Johnson, R. Draker, M. Ayers, T. Mazzulli, P. J. Talbot, and R. Tellier. 2004. Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay. J. Virol. Methods 122:29-36.
2. Bellini, W. J. 2003. SARS-CoV specific RT-PCR primers. [Online.] http: //www.who.int/csr/sars/CDCprimers.pdf.
3. Drosten, C., S. Gunther, W. Preiser, S. van der Werf, H. R. Brodt, S. Becker, H. Rabenau, M. Panning, L. Kolesnikova, R. A. Fouchier, A. Berger, A. M. Burguiere, J. Cinatl, M. Eickmann, N. Escriou, K. Grywna, S. Kramme, J. C. Manuguerra, S. Muller, V. Rickerts, M. Sturmer, S. Vieth, H. D. Klenk, A. D. Osterhaus, H. Schmitz, and H. W. Doerr. 2003. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N. Engl. J. Med. 348:1967-1976.
4. Drosten, C., L. L. Chiu, M. Panning, H. N. Leong, W. Preiser, J. S. Tam, S. Gunther, S. Kramme, P. Emmerich, W. L. Ng, H. Schmitz, and E. S. Koay. 2004. Evaluation of advanced reverse transcription-PCR assays and an alternative PCR target region for detection of severe acute respiratory syndrome-associated coronavirus. J. Clin. Microbiol. 42:2043-2047.
5. Hong, T. C., Q. L. Mai, D. V. Cuong, M. Parida, H. Minekawa, T. Notomi, F. Hasebe, and K. Morita. 2004. Development and evaluation of a novel loop-mediated isothermal amplification method for rapid detection of severe acute respiratory syndrome coronavirus. J. Clin. Microbiol. 42:1956-1961.
6. Houng, H. S., D. Norwood, G. V. Ludwig, W. Sun, M. Lin, and D. W. Vaughn. 2004. Development and evaluation of an efficient 3′-noncoding region based SARS coronavirus (SARS-CoV) RT-PCR assay for detection of SARS-CoV infections. J. Virol. Methods 120:33-40.
7. Hourfar, M. K., W. K. Roth, E. Seifried, and M. Schmidt. 2004. Comparison of two real-time quantitative assays for detection of severe acute respiratory syndrome coronavirus. J. Clin. Microbiol. 42:2094-2100.
8. Hui, R. K., F. Zeng, C. M. Chan, K. Y. Yuen, J. S. Peiris, and F. C. Leung. 2004. Reverse transcriptase PCR diagnostic assay for the coronavirus associated with severe acute respiratory syndrome. J. Clin. Microbiol. 42:1994-1999.
9. Mahony, J. B., A. Petrich, L. Louie, X. Song, S. Chong, M. Smieja, M. Chernesky, M. Loeb, and S. Richardson. 2004. Performance and cost evaluation of one commercial and six in-house conventional and real-time reverse transcription-PCR assays for detection of severe acute respiratory syndrome coronavirus. J. Clin. Microbiol. 42:1471-1476.
10. McIntosh, K. 2003. The SARS coronavirus: rapid diagnostics in the lime-light. Clin. Chem. 49:845-846.
11. Neyts, J., A. Meerbach, P. McKenna, and E. De Clercq. 1996. Use of the yellow fever virus vaccine strain 17D for the study of strategies for the treatment of yellow fever virus infections. Antivir. Res. 30:125-132.
12. Notomi, T., H. Okayama, H. Masubuchi, T. Yonekawa, K. Watanabe, N. Amino, and T. Hase. 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28:E63.
13. Poon, L. L., O. K. Wong, K. H. Chan, W. Luk, K. Y. Yuen, J. S. Peiris, and Y. Guan. 2003. Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS). Clin. Chem. 49:953-955.
14. Poon, L. L., C. S. Leung, M. Tashiro, K. H. Chan, B. W. Wong, K. Y. Yuen, Y. Guan, and J. S. Peiris. 2004. Rapid detection of the severe acute respiratory syndrome (SARS) coronavirus by a loop-mediated isothermal amplification assay. Clin. Chem. 50:1050-1052.
15. Rota, P. A., M. S. Oberste, S. S. Monroe, W. A. Nix, R. Campagnoli, J. P. Icenogle, S. Penaranda, B. Bankamp, K. Maher, M. H. Chen, S. Tong, A. Tamin, L. Lowe, M. Frace, J. L. DeRisi, Q. Chen, D. Wang, D. D. Erdman, T. C. Peret, C. Burns, T. G. Ksiazek, P. E. Rollin, A. Sanchez, S. Liffick, B. Holloway, J. Limor, K. McCaustland, M. Olsen-Rasmussen, R. Fouchier, S. Gunther, A. D. Osterhaus, C. Drosten, M. A. Pallansch, L. J. Anderson, and W. J. Bellini. 2003. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 300:1394-1399.
16. Ruan, Y. J., C. L. Wei, A. L. Ee, V. B. Vega, H. Thoreau, S. T. Su, J. M. Chia, P. Ng, K. P. Chiu, L. Lim, T. Zhang, C. K. Peng, E. O. Lin, N. M. Lee, S. L. Yee, L. F. Ng, R. E. Chee, L. W. Stanton, P. M. Long, and E. T. Liu. 2003. Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection. Lancet 361:1779-1785.
17. World Health Organization. 2003. Case definitions for surveillance of severe acute respiratory syndrome (SARS). [Online.] http://www.who.int/csr/sars /casedefinition/en/print.html.
18. World Health Organization. 2003. W.H.O. Scientific Research Advisory Committee on severe acute respiratory syndrome (SARS). Report of the first meeting, Geneva, Switzerland, 20-21 October, W.H.O./CDS/CSR/GAR /2004.16. World Health Organization, Geneva, Switzerland.
19. World Health Organization. 2003. Alert, verification and public health management of SARS in the post outbreak period. [Online.] http://www.who.int /csr/sars/postoutbreak/en/.