Rapid identification of potentially probiotic Bifidobacterium species by multiplex PCR using species-specific primers based on the region extending from 16S rRNA through 23S rRNA

FEMS Microbiology Letters

Volumn 250, Issue 1, 2005, Pages 55-62

  Kwon, Hyuk Sang ^a   Yang, Eun Hee ^a   Lee, Seung Hun ^a   Yeon, Seung Woo ^a   Kang, Byung Hwa ^a   Kim, Tae Yong ^a  

^a ILDONG Pharmaceutical Co   (South Korea)

Author keywords

16S ribosomal RNA; 23S ribosomal RNA; Bifidobacterium; Identification; Intergenic spacer region (ISR); Multiplex polymerase chain reaction (PCR); Probiotic; Species specific

Indexed keywords

BACTERIAL RNA; PRIMER RNA; PROBIOTIC AGENT; RIBOSOME RNA; SPACER DNA;

ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BIFIDOBACTERIUM; BIFIDOBACTERIUM ADOLESCENTIS; BIFIDOBACTERIUM ANIMALIS; BIFIDOBACTERIUM ANIMALIS LACTIS; BIFIDOBACTERIUM BIFIDUM; BIFIDOBACTERIUM BREVE; BIFIDOBACTERIUM LONGUM INFANTIS; BIFIDOBACTERIUM LONGUM LONGUM; BIFIDOBACTERIUM PSEUDOLONGUM; BIOASSAY; CONTROLLED STUDY; FECES; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RELIABILITY; REPRODUCIBILITY; RNA GENE; RNA SEQUENCE; SEQUENCE ANALYSIS; UNINDEXED SEQUENCE;

BASE SEQUENCE; BIFIDOBACTERIUM; DNA PRIMERS; DNA, BACTERIAL; DNA, RIBOSOMAL SPACER; FECES; HUMANS; INFANT; POLYMERASE CHAIN REACTION; PROBIOTICS; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; RNA, RIBOSOMAL, 23S; SPECIES SPECIFICITY;

BIFIDOBACTERIUM; BIFIDOBACTERIUM ADOLESCENTIS; BIFIDOBACTERIUM ANIMALIS; BIFIDOBACTERIUM BIFIDUM; BIFIDOBACTERIUM BREVE; BIFIDOBACTERIUM LONGUM; BIFIDOBACTERIUM LONGUM BIOVAR LONGUM; BIFIDOBACTERIUM PSEUDOLONGUM;

EID: 23744462797     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsle.2005.06.041     Document Type: Article

References (30)

1. J.P. Langhendries, J. Detry, J. van Hees, and J.M. Lamboray Effect of a fermented infant formula containing viable bifidobacteria on the fecal flora composition and pH of healthy full-term infants J. Pediatr. Gastroenterol. Nutr. 21 1995 177 181
2. J.M. Saavedra, A. Abi-Hanna, N. Moore, and R. Yolken Effect of long term consumption of infant formulas with bifidobacteria and S. thermophilus on stool pattern and diaper rash in infants J. Pediatr. Gastroenterol. Nutr. 27 1998 483 486
3. J.M. Saavedra, N.A. Baumann, and I. Oung Feeding of Bifidobacterium lactis and Streptococcus thermophilus to infant in hospital for prevention of diarrhea and shedding of rotavirus Lancet 334 1994 1046 1049
4. V. Scardovi Genus Bifidobacterium Orla-Jensen, 1924, 472 N.R. Krieg J.G. Holt Bergey's Manual of Systematic Bacteriology Vol. 1 1984 Williams and Wilkins Baltimore, MD 1418 1434
5. J. Ballongue Bifidobacteria and probiotic action S. Salminen A. Von Wright Lactic Acid Bacteria, Microbiology and Functional Aspects second ed. 1998 Marcel Dekkers, Inc Now York, NY 519 587
6. D. O'sullivan, and M.J. Kullen Tracking of probiotic bifidobacteria in the intestine Int. Dairy J. 8 1998 513 525
7. T. Matsuki, K. Watanabe, J. Fujimoto, Y. Miyamoto, T. Takada, K. Matsumoto, H. Oyaizu, and R. Tanaka Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces Appl. Environ. Microbiol. 68 2002 5445 5451
8. C. Mullie, M. Odou, E. shinger, M. Romond, and D. Izard Multiplex PCR using 16S rRNA gene-targeted primers for the identification of bifidobacteria from human origin FEMS Microbiol. Lett. 222 2003 129 136
9. M. Ventura, R. Reniero, and R. Zink Specific identification and targeted characterization of Bifidobacterium lactis from different environmental isolates by a combined multiplex-PCR approach Appl. Environ. Microbiol. 67 2001 2760 2765
10. G. Muyzer, E.C. De Waal, and A.G. Uitterlinden Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA Appl. Environ. Microbiol. 59 1993 695 700
11. D. Roy, P. Ward, and G. Champagne Differentiation of bifidobacteria by use of pulse-field gel electrophoresis and polymerase chain reaction Int. J. Food Microbiol. 29 1996 11 29
12. R.M. Stocari, E.E. Vaughan, A.D.L. Akkermans, M. Saarela, and W.M. De Vos Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis Appl. Environ. Microbiol. 67 2001 504 513
13. D. Vincent, D. Roy, F. Mondou, and C. Dery Characterization of bifidobacteria by random DNA amplification Int. J. Food Microbiol. 43 1998 185 193
14. R.I. Amann, W. Ludwig, and K.H. Schleifer Phylogenetic identification and in situ detection of individual microbial cells without cultivation Microbiol. Rev. 59 1995 143 169
15. N.L. Bourget, H. Philippe, I. Mangin, and B. Decaris 16S rRNA and 16S to 23S internal transcribed spacer sequence analysis reveal inter- and intraspecific Bifidobacterium phylogeny Int. J. Syst. Bacteriol. 46 1996 102 111
16. T. Matsuki, K. Watanabe, R. Tanaka, M. Fukuda, and H. Oyaizu Distribution of bifidobacterial species in human intestinal microflora examined with 16S rRNA-gene-targeted species-specific primers Appl. Environ. Microbiol. 65 1999 4506 4512
17. A. Tilsala-Timisjarvi, and T. Alatossava Development of oligonucleotide primers from the 16S-23S intergenic sequences for identifying different dairy and probiotic lactic acid bacteria by PCR Int. J. Food Microbiol. 35 1997 49 56
18. M. Ventura, and R. Zink Rapid identification, differentiation, and proposed new taxonomic classification of Bifidobacterium lactis Appl. Environ. Microbiol. 68 2002 6429 6434
19. H.-S. Kwon, E.-H. Yang, S.-W. Yeon, B.-H. Kang, and T.-Y. Kim Rapid identification of probiotic Lactobacillus species by multiplex PCR using species-specific primers based on the region extending from 16S rRNA through 23S rRNA FEMS Microbiol. Lett. 239 2004 267 275
20. R. Temmerman, B. Pot, G. Huys, and J. Swings Identification and antibiotic susceptibility of bacterial isolates from probiotic products Int. J. Food Microbiol. 81 2003 1 10
21. M.J. Kullen, L.J. Brady, and D.J. O'Sullivan Evaluation of using a short region of the RecA gene for rapid and sensitive speciation of dominant bifidobacteria in the human large intestine FEMS Microbiol. Lett. 154 1997 377 383
22. T. Matsuki, K. Watanabe, R. Tanaka, and H. Oyaizu Rapid identification of human intestinal bifidobacteria by 16S rRNA-targeted species- and group-specific primers FEMS Microbiol. Lett. 167 1998 113 121
23. T. Requena, J. Burton, T. Matsuki, K. Munro, M.A. Simon, R. Tanaka, K. Watanabe, and G.W. Tannock Identification, detection, and enumeration of human Bifidobacterium species by PCR targeting the transaldolase gene Appl. Environ. Microbiol. 68 2002 2420 2427
24. N. Leblong-Bourget, H. Philippe, I. Mangin, and B. Decaris 16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny Int. J. Syst. Bacteriol. 46 1996 564 571
25. Y. Cai, M. Matsumoto, and Y. Benno Bifidobacterium lactis Meile et al. 1997 is a subjective synonym of Bifidobacterium animalis (Mitsuoka 1969) Scardovi and Trovatelli 1974 Microbiol. Immunol. 44 2000 815 820
26. L. Masco, M. Ventura, R. Zink, G. Huys, and J. Swings Polyphasic taxonomic analysis of Bifidobacterium animalis and Bifidobacterium lactis reveals relatedness at the subspecies level: reclassification of Bifidobacterium animalis as Bifidobacterium animalis subsp. animalis subsp. nov. and Bifidobacterium lactis as Bifidobacterium animalis subsp. lactis subsp. nov Int. J. Syst. Evol. Microbiol. 54 2004 1137 1143
27. S. Sakata, M. Kitahara, M. Sakamoto, H. Hayashi, M. Fukuyama, and Y. Benno Unification of Bifidobacterium infantis and Bifidobacterium suis as Bifidobacterium longum Int. J. Syst. Evol. Microbiol. 52 2002 1945 1951
28. F. Gavini, M. van Esbroeck, J.P. Touzel, A. Fourment, and H. Goosens Detection of fructose 6-phosphate phosphoketolase (F6PPK), a key enzyme in the bifid-shunt, in Gardnerella vaginalis Anaerobe 2 1996 191 193
29. W. Jian, and X. Dong Transfer of Bifidobacterium inopinatum and Bifidobacterium denticolens to Scardovia inopinata gen. nov., comb. nov., and Parascardovia denticolens gen. nov., comb. nov., respectively Int. J. Syst. Evol. Microbiol. 52 2002 809 812
30. J. Brosius, T.J. Dull, D.D. Sleeter, and H.F. Noller Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli J. Mol. Biol. 148 1981 107 127