Effects of fixation and decalcification on the immunohistochemical localization of bone matrix proteins in fresh-frozen bone sections

Histochemistry and Cell Biology

Volumn 123, Issue 6, 2005, Pages 639-646

  Hosoya, Akihiro ^a   Hoshi, Kazuto ^b   Sahara, Noriyuki ^a   Ninomiya, Tadashi ^a   Akahane, Syoji ^a   Kawamoto, Tadafumi ^c   Ozawa, Hidehiro ^a  

^a MATSUMOTO DENTAL UNIVERSITY   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c TSURUMI UNIVERSITY   (Japan)

Author keywords

Bone; Bone matrix proteins; Fresh frozen section; Immunohistochemistry; Non decalcified section

Indexed keywords

APATITE; CALCIUM; COLLAGEN TYPE 1; EDETIC ACID; OSTEOPONTIN; PARAFORMALDEHYDE; PHOSPHORUS; SIALOPROTEIN;

ANIMAL TISSUE; ARTICLE; BONE MATRIX; CONTROLLED STUDY; ELECTRON PROBE MICROANALYSIS; ENVIRONMENT; FEMUR; HISTOLOGY; IMMUNOHISTOCHEMISTRY; IMMUNOLOCALIZATION; MICRORADIOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; TISSUE FIXATION;

ANIMALS; BONE MATRIX; COLLAGEN TYPE I; DECALCIFICATION TECHNIQUE; EDETIC ACID; ELECTRON PROBE MICROANALYSIS; FEMUR; FORMALDEHYDE; FROZEN SECTIONS; IMMUNOHISTOCHEMISTRY; MICRORADIOGRAPHY; OSTEOPONTIN; POLYMERS; RATS; RATS, WISTAR; SIALOGLYCOPROTEINS; STAINING AND LABELING; TISSUE FIXATION;

EID: 23744442370     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00418-005-0791-4     Document Type: Article

References (34)

1. Arafat HA, Wein AJ, Chacko S (2002) Osteopontin gene expression and immunolocalization in the rabbit urinary tract. J Urol 167:746-752
2. Baker JR, Hew H, Fishman WH (1958) The use of a chloral hydrate formaldehyde fixative solution in enzyme histochemistry. J Histochem Cytochem 6:244-250
3. Begue-Kirn C, Smith AJ, Loriot M, Kupferle C, Ruch JV, Lesot H (1994) Comparative analysis of TGF beta s, BMPs, IGF1, msxs, fibronectin, osteonectin and bone sialoprotein gene expression during normal and in vitro-induced odontoblast differentiation. Int J Dev Biol 38:405-420
4. Beninati S, Senger DR, Cordella-Miele E, Mukherjee AB, Chackalaparampil I, Shanmugam V, Singh K, Mukherjee BB (1994) Osteopontin: its transglutaminase-catalyzed posttranslational modifications and cross-linking to fibronectin. J Biochem 115:675-682
5. Bosshardt DD, Nanci A (1998) Immunolocalization of epithelial and mesenchymal matrix constituents in association with inner enamel epithelial cells. J Histochem Cytochem 46:135-142
6. Bronckers AL, Farach-Carson MC, Van Waveren E, Butler WT (1994) Immunolocalization of osteopontin, osteocalcin, and dentin sialoprotein during dental root formation and early cementogenesis in the rat. J Bone Miner Res 9:833-841
7. Fink S (1992) A solvent-free coating-procedure for the improved preparation of cryostat section in light microscope histochemistry. Histochemistry 97:243-246
8. Fukase Y (1997) Immunohistochemical staining of human teeth, and production of monoclonal antibodies against cementum. J Osaka Dent Univ 31:1-9
9. Hammarstrom L (1986) Autoradiography and histochemistry of mineralized tissues by means of the Ullberg freeze-sectioning technique. Upsala J Med Sci 91:239-243
10. Helder MN, Bronckers AL, Woltgens JH (1993) Dissimilar expression patterns for the extracellular matrix proteins osteopontin (OPN) and collagen type I in dental tissues and alveolar bone of the neonatal rat. Matrix 13:415-425
11. Hill EL, Elde R (1990) An improved method for preparing cryostat sections of non-decalcified bone for multiple use. J Histocem Cytochem 38:443-448
12. Hoshi K, Ejiri S, Ozawa H (2001) Organic components of crystal sheaths in bones. J Electron Microsc 50:33-40
13. Hunter GK, Hauschka PV, Poole AR, Rosenberg LC, Goldberg HA (1996) Nucleation and inhibition of hydroxyapatite formation by mineralized tissue proteins. Biochem J 317:59-64
14. Kaartinen MT, EL-Maadawy S, Rasanen NH, Mckee MD (2002) Tissue transglutaminase and its substrates in bone. J Bone Miner Res 17:2161-2173
15. Kawamoto T (2003) Use of a new adhesive film for the preparation of multi-purpose fresh-frozen sections from hard tissues, whole-animals, insects and plants. Arch Histol Cytol 66:123-143
16. Kawamoto T, Shimizu M (2000) A method for preparing 2- to 50-μm-thick fresh-frozen sections of large samples and undecalcified hard tissues. Histochem Cell Biol 113:331-339
17. Macneil RL, Sheng N, Strayhorn C, Fisher LW, Somerman MJ (1994) Bone sialoprotein is localized to the root surface during cementogenesis. J Bone Miner Res 9:1597-1606
18. Mark MP, Butler WT, Prince CW, Finkelman RD, Ruch JV (1988) Developmental expression of 44-kDa bone phosphoprotein (osteopontin) and bone gamma-carboxyglutamic acid (Gla)-containing protein (osteocalcin) in calcifying tissues of rat. Differentiation 37:123-136
19. McElroy HH, Shin M-S, Parfitt AM (1993) Producing frozen sections of calcified bone. Biotech Histochem 68:50-55
20. McKee MD, Farach-Carson MC, Butler WT, Hauschka PV, Nanci A (1993) Ultrastructural immunolocalization of noncollagen-ous (osteopontin and osteocalcin) and plasma (albumin and alpha 2HS-glycoprotein) proteins in rat bone. J Bone Miner Res 8:485-496
21. Miyazaki Y, Setoguchi M, Yoshida S, Higuchi Y, Akizuki S (1990) The mouse osteopontin gene. Expression in monocytic lineages and complete nucleotide sequence. J Biol Chem 265:14432-14438
22. Mukai K, Yoshimura S, Anzai M (1986) Effects of decalcification on immunoperoxidase staining. Am J Surg Pathol 10:413-719
23. Mullink H, Henzen-Logmans SC, Tadama TM, Mol JJ, Meijer CJ (1985) Influence of fixation and decalcification on the immunohistochemical staining of cell-specific markers in paraffin-embedded human bone biopsies. J Histochem Cytochem 33:1103-1109
24. Nanci A (2003) Ten Cate's oral histology: development, structure, and function, 6th edn. Mosby, St Louis, pp 61-71
25. Patarca R, Freeman GJ, Sing RP, Wei FY, Durfee T, Blottner F, Regnier DC, Kozak CA, Mock BA, Morse HC (1986) Structual and functional studies of the early T-lymphocyte activation 1 (Eta-1) gene. Definition of a novel T-cell dependent response associated with genetic resistance to bacterial injection. J Exp Med 170:145-161
26. Shimada M, Watanabe M (1995) Recent progress in whole-body radiography. Cell Mol Biol 41:39-48
27. Takeshita N, Kuwahara S, Shirasuga H, Akiba M, Nagai N (1983) The influence of decalcifying solution on immunoperoxidase staining (PAP) in paraffin section. Jpn J Oral Biol 25:1134-1135
28. Tripp EJ, Mackay EH (1972) Silver staining of bone prior to decalcification for quantitative determination of osteoid in sections. Stain Technol 47:129-136
29. Van Noorden CJ, Vogels IM (1986) Enzyme histochemical reactions in unfixed and undecalcified cryostat sections of mouse knee joints with special reference to arthritic lesions. Histochemistry 86:127-133
30. Van Noorden CJ, Vogels IM, Van Wering ER (1989) Enzyme cytochemistry of unfixed leukocytes and bone marrow cells using polyvinyl alcohol for the diagnosis of leukemia. Histochemistry 92:313-318
31. Van Den Munckhof RJM, Vreeling-Sinderarova H, Schellens JPM, Frederiks WM (1994) Localization of uric acid oxidase activity in core and matrix of peroxisomes as detected in unfixed cryostat sections of rat liver unfixed. J Histochem Cytochem 42:177-183
32. Villanueva AR, Mehr LA (1977) Modifications of the Goldner and Gomori one-step Trichrome stains for plastic-embedded thin sections of bone. Am J Med Technol 43:536-538
33. Wakisaka S (1986) Immunohistochemical study on substance P in rat molar pulp and periodontal tissues. I. Effect of decalcifying agents on substance P-like immunoreactivity. J Osaka Univ Dental Soc 31:203-208
34. Yoshiki S (1973) A simple histological method for identification of osteoid matrix in decalcified bone. Stain Technol 48:233-238