Fucan inhibits Chinese hamster ovary cell (CHO) adhesion to fibronectin by binding to the extracellular matrix

Planta Medica

Volumn 71, Issue 7, 2005, Pages 628-633

  Rocha, Hugo A O ^a,b   Franco, Célia R C ^c   Trindade, Edvaldo S ^b   Veiga, Silvio S ^c   Leite, Edda L ^a   Nader, Helena B ^b   Dietrich, Carl P ^b  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE   (Brazil)

^b FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

^c FEDERAL UNIVERSITY OF PARANÁ   (Brazil)

Author keywords

Anti adhesive fucan; Cell adhesion; CHO cells; Fucoidan; Phaeophyceae; Spatoglossum schr ederi

Indexed keywords

BIOPOLYMER; FIBRONECTIN; FRUCTOSE; FUCAN; POLYSACCHARIDE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING SITE; BIOTINYLATION; CELL ADHESION; CHO CELL; CONCENTRATION RESPONSE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DRUG ACTIVITY; EXTRACELLULAR MATRIX; FLOW CYTOMETRY; NONHUMAN; PROTEIN BINDING; SEAWEED;

ANIMALS; ANTICOAGULANTS; CELL ADHESION; CHO CELLS; CRICETINAE; CRICETULUS; DISACCHARIDES; DOSE-RESPONSE RELATIONSHIP, DRUG; EXTRACELLULAR MATRIX; FEMALE; FIBRONECTINS; FLOW CYTOMETRY; PHYTOTHERAPY; PLANT EXTRACTS; POLYSACCHARIDES; SEAWEED;

ALGAE; CRICETULUS GRISEUS; PHAEOPHYCEAE; SPATOGLOSSUM SCHROEDERI;

EID: 23644442709     PISSN: 00320943     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2005-871268     Document Type: Article

References (15)

1. Dietrich CP, Farias GGM, Abreu LRD, Leite EL, Silva LF, Nader HB. A new approach for characterization of polysaccharides from algae: Presence of four main acidic polysaccharides in three species of the class Phaeophycea. Plant Sci 1995; 108: 143-53
2. Vilela-Silva ESA, Castro MO, Valente AP, Biermann HC, Mourão PAS. Sulfated fucans from the egg jellies of the closely related sea urchins Strongylocentrotus droebachiensis and Strongylocentrotus pallidus ensure species-specific fertilization. J Biol Chem 2002; 277: 379-87
3. Soeda S, Ohmagugi Y, Shimeno H, Nagamatsu A. Preparation of aminated fucoidan and its evaluation as an antithrombotic and antilipemic agent. Biol Pharmac Bull 1994; 17: 784-8
4. Liu JM, Haroun-Bouhedja F, Boisson-Vidal C. Analysis of the in vitro inhibition of mammary adenocarcinoma cell adhesion by sulfated polysaccharides. Anticancer Res 2000; 20: 3265-72
5. Leite EL, Medeiros MGL, Rocha HAO, Farias GGM, Silva LF, Chavante SF et al. Structure and pharmacological activities of sulfated xylofucoglucuronan from the alga Spatoglossum schroederi. Plant Sci 1998; 132: 215-28
6. Rocha HAO, Franco CRC, Trindade ES, Carvalho LCM, Veiga SS, Leite EL et al. A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins. Braz J Med Biol Res 2001; 34: 621-6
7. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem 1956; 28: 250-6
8. Nader HB, Dietrich CP. Determination of sulfate after chromatography and toluidine blue complexation. Anal Biochem 1977; 78: 112-8
9. Spector J. Refinement of the coomassie blue method of protein quantification. A simple and linear spectrophotometric assay of 0.5 to 50 μg of protein. Anal Biochem 1978; 86: 142-3
10. Kozakai M, Yosizawa Z. A rapid method for separation and identification of several hexuronic acids and hexuronic acid-containing oligosaccharides. Anal Biochem 1975; 78: 425-9
11. Trindade ES, Oliver C, Jamur MC, Franco CRC, Gouveia TC, Dietrich CP, Nader HB. Biotinylated heparin stimulates heparan sulfate production without binding the cell surface. Mol Biol Cell 1999; 10 (n.S.): 459
12. Rapraeger AC, Krufka A, Bradley BO. Requirement of heparan sulfate for βFGF-mediated fibroblast growth and myoblast differentiation. Science 1991; 252: 1705-7
13. Raimondi L, Banchelli G, Dalmazzi D, Mulinacci N, Romani A, Vincieri FF et al. Sedum telephium I. polysaccharide content affects MRC5 cell adhesion to laminin and fibronectin. J Pharm Pharmacol 2000; 20: 585-91
14. Esko JD, Rostand KS, Weinke JL. Tumor formation dependent on proteoglycan biosynthesis. Science 1988; 241: 1092-6
15. Franco CRC, Rocha HAO, Trindade ES, Santos IAN, Leite EL, Veiga SS et al. Heparan sulfate and control of cell division: adhesion and proliferation of mutant CHO-745 cells lacking xylosyl transferase. Braz J Med Biol Res 2001; 34: 971-5