Multiplexed analysis of biomarkers related to obesity and the metabolic syndrome in human plasma, using the Luminex-100 system

Clinical Chemistry

Volumn 51, Issue 7, 2005, Pages 1102-1109

  Liu, Mine Y ^a   Xydakis, Antonios M ^a   Hoogeveen, Ron C ^a   Jones, Peter H ^a   Smith, E O'Brian ^a   Nelson, Kathleen W ^b   Ballantyne, Christie M ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b HOUSTON METHODIST HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADIPOCYTOKINE; BIOLOGICAL MARKER; C PEPTIDE; EOTAXIN; INSULIN; INTERLEUKIN 6; INTERLEUKIN 8; LEPTIN; MONOCYTE CHEMOTACTIC PROTEIN 1; PROTEIN; TUMOR NECROSIS FACTOR ALPHA; CCL2 PROTEIN, HUMAN; CYTOKINE; FLUORESCENT DYE; MICROSPHERE;

ADULT; ARTICLE; CARDIOVASCULAR DISEASE; CONTROLLED STUDY; CORRELATION ANALYSIS; CORRELATION COEFFICIENT; DIABETES MELLITUS; DIET; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; FLUORESCENCE ANALYSIS; HUMAN; IMMUNOASSAY; INSULIN BLOOD LEVEL; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; METABOLIC SYNDROME X; OBESITY; PROTEIN ANALYSIS; PROTEIN BLOOD LEVEL; PROTEIN EXPRESSION; RISK FACTOR; VALIDATION PROCESS; WEIGHT REDUCTION; ADIPOSE TISSUE; BLOOD; COMPARATIVE STUDY; METABOLISM; MIDDLE AGED;

ADIPOSE TISSUE; BIOLOGICAL MARKERS; CARDIOVASCULAR DISEASES; CHEMOKINE CCL2; CYTOKINES; FEMALE; FLUORESCENT DYES; HUMANS; IMMUNOASSAY; INSULIN; INTERLEUKIN-6; INTERLEUKIN-8; LEPTIN; MALE; METABOLIC SYNDROME X; MICROSPHERES; MIDDLE AGED; OBESITY; RISK FACTORS; TUMOR NECROSIS FACTOR-ALPHA; WEIGHT LOSS;

EID: 23444453074     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2004.047084     Document Type: Article

References (20)

1. Flegal KM, Carroll MD, Ogden CL, Johnson CL. Prevalence and trends in obesity among US adults, 1999-2000. JAMA 2002;288:1723-7.
2. Moller DE, Flier JS. Insulin resistance-mechanisms, syndromes, and implications. N Engl J Med 1991;325:938-48.
3. Hubert HB, Feinleib M, McNamara PM, Castelli WP. Obesity as an independent risk factor for cardiovascular disease: a 26-year follow-up of participants in the Framingham Heart Study. Circulation 1983;67:968-77.
4. Loskutoff DJ, Samad F. The adipocyte and hemostatic balance in obesity. Studies of PAI-1. Arterioscler Thromb Vasc Biol 1998;18:1-6.
5. Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults. Executive summary of the third report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III). JAMA 2001;285:2486-97.
6. Ford ES, Giles WH, Dietz WH. Prevalence of the metabolic syndrome among US adults: findings from the third National Health and Nutrition Examination Survey. JAMA 2002;287:356-9.
7. Tarnok A, Hambsch J, Chen R, Varro R. Cytometric bead array to measure six cytokines in twenty-five microliters of serum. Clin Chem 2003;49:1000-2.
8. Prabhakar U, Eirikis E, Davis HM. Simultaneous quantification of proinflammatory cytokines in human plasma using the LabMAP assay. J Immunol Methods 2002;260:207-18.
9. Kellar KL, Kalwar RR, Dubois KA, Crouse D, Chafin WD, Kane BE. Multiplexed fluorescent bead-based immunoassays for quantitation of human cytokines in serum and culture supernatants. Cytometry 2001;45:27-36.
10. Vignali DAA. Multiplexed particle-based flow cytometric assays. J Immunol Methods 2000;243:243-55.
11. Oliver KG, Kettman JR, Fulton RJ. Multiplexed analysis of human cytokines by use of the FlowMetrix system. Clin Chem 1998;44:2057-60.
12. Case CC, Jones PH, Nelson K, Smith EO, Ballantyne CM. Impact of weight loss on the metabolic syndrome. Diabetes Obes Metab 2002;4:407-14.
13. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986;1:307-10.
14. Marks V. False-positive immunoassay results: a multicenter survey of erroneous immunoassay results from assays of 74 analytes in 10 donors from 66 laboratories in seven countries. Clin Chem 2002;48:2008-16.
15. Kaplan IV, Levinson SS. When is a heterophile antibody not a heterophile antibody? When it is an antibody against a specific immunogen. Clin Chem 1999;45:616-8.
16. Biagini RE, Sammons DL, Smith JP, MacKenzie BA, Striley CA, Semenova V, et al. Comparison of a multiplexed fluorescent covalent microsphere immunoassay and an enzyme-linked immunosorbent assay for measurement of human immunoglobulin G antibodies to anthrax toxins. Clin Diagn Lab Immunol 2004;11:50-5.
17. de Jager W, te Velthuis H, Prakken BJ, Kuis W, Rijkers GT. Simultaneous detection of 15 human cytokines in a single sample of stimulated peripheral blood mononuclear cells. Clin Diagn Lab Immunol 2003;10:133-9.
18. Jiang B, Snipes-Magaldi L, Dennehy P, Keyserling H, Holman RC, Bresee J, et al. Cytokines as mediators for or effectors against rotavirus disease in children. Clin Diagn Lab Immunol 2003;10:995-1001.
19. Nelson KB, Grether JK, Dambrosia JM, Walsh E, Kohler S, Satyanarayana G, et al. Neonatal cytokines and cerebral palsy in very preterm infants. Pediatr Res 2003;53:600-7.
20. Wadhwa M, Thorpe R. Cytokine immunoassays: recommendations for standardisation, calibration and validation. J Immunol Methods 1998;219:1-5.