Protein-trap version 2.1: Screening for expressed proteins in mammalian cells based on their localizations

BMC Cell Biology

Volumn 5, Issue , 2004, Pages

  Sineshchekova, Olga O ^a   Kawate, Toshimitsu ^a   Vdovychenko, Oleksandr V ^a   Sato, Thomas N ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOVIRUS VECTOR; CELL PROTEIN; EPITOPE; GENOMIC DNA; GREEN FLUORESCENT PROTEIN; PUROMYCIN;

ANALYTIC METHOD; ARTICLE; CANCER CELL CULTURE; CELL SCREENING; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DEVELOPMENT; DROSOPHILA; DRUG IDENTIFICATION; DRUG RESISTANCE; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSED SEQUENCE TAG; EXPRESSION VECTOR; GENE IDENTIFICATION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; LUNG CARCINOMA; MAMMAL CELL; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN EXPRESSION; SOUTHERN BLOTTING; WESTERN BLOTTING;

ANTIBODIES, MONOCLONAL; BLOTTING, WESTERN; CARCINOMA; CLONING, MOLECULAR; EPITOPES; EXONS; EXTRACELLULAR MATRIX; GENETIC VECTORS; HUMANS; INTRACELLULAR SPACE; LUNG NEOPLASMS; PROTEIN BIOSYNTHESIS; PROTEIN TRANSPORT; PROTEINS; RECOMBINANT FUSION PROTEINS; RETROVIRIDAE; SIGNAL TRANSDUCTION; TUMOR CELLS, CULTURED;

MAMMALIA;

EID: 2342659642     PISSN: 14712121     EISSN: None     Source Type: Journal    
DOI: 10.1186/1471-2121-5-8     Document Type: Article

References (26)

1. Wan JS, Sharp SJ, Poirier GM, Wagaman PC, Chambers J, Pyati J, Hom YL, Galindo JE, Huvar A, Peterson PA, Jackson MR, Erlander MG: Cloning differentially expressed mRNAs. Nat Biotechnol 1996, 14:1685-1691.
2. Claverie JM: Computational methods for the identification of differential and coordinated gene expression. Hum Mol Genet 1999, 8:1821-1832.
3. Diatchenko L, Lau YF, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD: Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc Natl Acad Sci U S A 1996, 93:6025-6030.
4. Stanford WL, Cohn JB, Cordes SP: Gene-trap mutagenesis: past, present and beyond. Nat Rev Genet 2001, 2:756-768.
5. Joyner AL: Gene targeting and gene trap screens using embryonic stem cells: new approaches to mammalian development. Bioessays 1991, 13:649-656.
6. Lai Z, Han I, Park M, Brady RO: Design of an HIV-1 lentiviralbased gene-trap vector to detect developmentally regulated genes in mammalian cells. Proc Natl Acad Sci U S A 2002, 99:3651-3656.
7. Skarnes WC, Moss JE, Hurtley SM, Beddington RS: Capturing genes encoding membrane and secreted proteins important for mouse development. Proc Natl Acad Sci U S A 1995, 92:6592-6596.
8. Jarvik JW, Adler SA, Telmer CA, Subramanian V, Lopez AJ: CD-tagging: A new approach to gene and protein discovery and analysis. BioTechniques 1996, 20:896-904.
9. Smith DJ: Mini-exon epitope tagging for analysis of the protein coding protential of genomic sequence. BioTechniques 1997, 23:116-120.
10. Cutler SR, Ehrhardt DW, Griffitts JS, Somerville CR: Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency. Proc Natl Acad Sci U S A 2000, 97:3718-3723.
11. Misawa K, Nosaka T, Morita S, Kaneko A, Nakahata T, Asano S, Kitamura T: A method to identify cDNAs based on localization of green fluorescent protein fusion products. Proc Natl Acad Sci U S A 2000, 97:3062-3066.
12. Ding DQ, Tomita Y, Yamamoto A, Chikashige Y, Haraguchi T, Hiraoka Y: Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library. Genes Cells 2000, 5:169-190.
13. Morin X, Daneman R, Zavortink M, Chia W: A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila. Proc Natl Acad Sci U S A 2001, 98:15050-15055.
14. Griffin BA, Adams SR, Tsien RY: Specific covalent labeling of recombinant protein molecules inside live cells. Science 1998, 281:269-272.
15. Semenza GL: Perspectives on oxygen sensing. Cell 1999, 98:281-284.
16. Semenza GL: Hypoxia-inducible factor 1: master regulator of O2 homeostasis. Curr Opin Genet Dev 1998, 8:588-594.
17. Semenza GL: Expression of hypoxia-inducible factor 1: mechanisms and consequences. Biochem Pharmacol 2000, 59:47-53.
18. Bruick RK: Expression of the gene encoding the proapoptotic Nip3 protein is induced by hypoxia. Proc Natl Acad Sci U S A 2000, 97:9082-9087.
19. Seta KA, Kim R, Kim HW, Millhorn DE, Beitner-Johnson D: Hypoxia-induced regulation of MAPK phosphatase-1 as identified by subtractive suppression hybridization and cDNA microarray analysis. J Biol Chem 2001, 276:44405-44412.
20. Scandurro AB, Weldon CW, Figueroa YG, Alam J, Beckman BS: Gene microarray analysis reveals a novel hypoxia signal transduction pathway in human hepatocellular carcinoma cells. Int J Oncol 2001, 19:129-135.
21. Ivics Z, Hackett PB, Plasterk RH, Izsvak Z: Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Cell 1997, 91:501-510.
22. Kinsella TM, Nolan GP: Episomal vectors rapidly and stably produce high-titer recombinant retrovirus. Hum Gene Ther 1996, 7:1405-1413.
23. O'Gorman S, Dagenais NA, Qian M, Marchuk Y: Protamine-Cre recombinase transgenes efficiently recombine target sequences in the male germ line of mice, but not in embryonic stem cells. Proc Natl Acad Sci U S A 1997, 94:14602-14607.
24. Grignani F, Kinsella T, Mencarelli A, Valtieri M, Riganelli D, Lanfrancone L, Peschle C, Nolan GP, Pelicci PG: High-efficiency gene transfer and selection of human hematopoietic progenitor cells with a hybrid EBV/retroviral vector expressing the green fluorescence protein. Cancer Res 1998, 58:14-19.
25. Brogi E, Schatteman G, Wu T, Kim EA, Varticovski L, Keyt B, Isner JM: Hypoxia-induced paracrine regulation of vascular endothelial growth factor receptor expression. J Clin Invest 1996, 97:469-476.
26. Squinto SP, Aldrich TH, Lindsay RM, Morrissey DM, Panayotatos N, Bianco SM, Furth ME, Yancopoulos GD: Identification of functional receptors for ciliary neurotrophic factor on neuronal cell lines and primary neurons. Neuron 1990, 5:757-766.