Development of non-electrophoretic assay method for DNA ligases and its application to screening of chemical inhibitors of DNA ligase I

Journal of Biochemical and Biophysical Methods

Volumn 59, Issue 1, 2004, Pages 49-59

  Sun, Daekyu ^a   Urrabaz, Rheanna ^a  

^a CTRC Institute for Drug Development   (United States)

Author keywords

DNA ligase assay; DNA ligase inhibitor; DNA ligases

Indexed keywords

2 (3,5 DIHYDROXYPHENYL) 2 METHYLDECALIN; 2 (3,5 DIHYDROXYPHENYL) 4 ETHYL 1 METHYLCYCLOHEXANE; 5 (1 ADAMANTYL)RESORCINOL; ENZYME INHIBITOR; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE I; POLYDEOXYRIBONUCLEOTIDE SYNTHASE I INHIBITOR; RESORCINOL; RESORCINOL DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; BIOTINYLATION; CONTROLLED STUDY; CORRELATION ANALYSIS; DNA DENATURATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; HEAT TREATMENT; HUMAN; HUMAN CELL; MICROTITER PLATE ASSAY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; STANDARD;

BIOTINYLATION; DNA LIGASES; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; GENE LIBRARY; HUMANS; ISOTOPE LABELING; METHODS; OLIGONUCLEOTIDES; PHOSPHORUS RADIOISOTOPES;

MINK CELL FOCUS-FORMING VIRUS;

EID: 2342657883     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(02)00071-4     Document Type: Article

References (22)

1. Soderhall S., Lindahl T. Mammalian DNA ligases: serological evidence for two separate enzymes. J. Biol. Chem. 250:1975;8438-8444
2. Lindahl T., Branes D.E. Mammalian DNA ligases. Annu. Rev. Biochem. 269:1992;251-281
3. Tomkinson A.E., Chen J., Besterman J., Husain I. Cellular functions of mammalian DNA ligases. Nickoloff J.A., Hoekstra M.F. DNA damage and repair. DNA repair in higher eukaryotes. vol. II:1998;181-198 Humana Press, Totowa, NJ
4. Lehman I.R. DNA ligase: structure, mechanism, and function. Science. 186:1974;790-797
5. Lasko D.D., Tomkinson A.E., Lindahl T. Mammalian DNA ligases. Biosynthesis and intracellular localization of DNA ligase I. J. Biol. Chem. 265:1990;12618-12622
6. Chen J., Tomkinson A.E., Ramos W., Mackey Z.B., Danehower S., Walter C.A., et al. Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination. Mol. Cell. Biol. 15:1995;5412-5422
7. Wei Y.F., Robins P., Carter K., Caldecott K., Pappin D.J., Yu G.L., et al. Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination. Mol. Cell. Biol. 15:1995;3206-3216
8. Robins P., Lindahl T. DNA ligase IV from HeLa cell nuclei. J. Biol. Chem. 144:1996;24257-24261
9. Prigent C., Satoh M.S., Daly G., Barnes D.E., Lindahl T. Aberrant DNA repair and DNA replication due to an inherited enzymatic defect in human DNA ligase I. Mol. Cell. Biol. 14:1994;310-317
10. Waga S., Bauer G., Stillman B. Reconstitution of complete SV40 DNA replication with purified replication factor. J. Biol. Chem. 269:1997;10923-10934
11. Congjun L., Goodchild J., Baril E.F. DNA ligase I is associated with 21S complex of enzymes for DNA synthesis in HeLa cells. Nucleic Acids Res. 22:1994;632-638
12. Barnes D.E., Tomkinson A.E., Lehmann A.R., Webster A.D.B., Lindahl T. Mutations in the DNA ligase I gene of an individual with immunodeficiencies and cellular hypersensitivity to DNA-damaging agents. Cell. 69:1992;495-503
13. Chan J.Y.H., Becker F.F. DNA ligase activities during hepatocarcinogenesis induced by N-2-acetylaminofluorene. Carcinogenesis. 6:1985;1275-1277
14. Montecucco A., Savini E., Biamonti G., Stefanini M., Focher F., Ciarrocchi G. Late induction of human DNA ligase I after UV-C irradiation. Nucleic Acids Res. 23:1995;962-966
15. Signoret J., David J.C. Control of the expression of genes for DNA ligase in eukaryotes. Int. Rev. Cytol. 103:1986;249-279
16. Montecucco A., Biamonti G., Savini E., Focher F., Spadari S., Ciarrocchi G. DNA ligase I gene expression during differentiation and cell proliferation. Nucleic Acids Res. 20:1992;6209-6214
17. Teraoka H., Sawai M., Yamamoto K., Tsukada K. DNA ligase I mRNA and enzyme levels in human hematopoietic cells under dimethyl sulfoxide-induced growth-arrest and differentiation. Biochem. Int. 26:1992;963-971
18. Bentley D., Selfridge J., Millar J.K., Samuel K., Hole N., Ansell J.D., et al. DNA ligase I is required for fetal liver erythropoiesis but is not essential for mammalian cell viability. Nat. Genet. 13:1996;489-491
19. Sun D., Urrabaz R.M., Nguyen M., Marty J., Stringer S., Cruz E., et al. Elevated expression of DNA ligase I in human cancers. Clin. Cancer Res. 2001;. [in press]
20. Mossman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65:1983;55-63
21. Givens J.F., Manly K.F. Inhibition of RNA-directed DNA polymerase by aurintricarboxylic acid. Nucleic Acids Res. 3:1976;405-418
22. Lakshmipathy U., Campbell C. Antisense-mediated decrease in DNA ligase III expression results in reduced mitochondrial DNA integrity. Nucleic Acids Res. 29:2001;668-676